You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial...
Homework Answers
The dilution factor is the final dilution that is used to get the colonies which are in the range of 30-300. So the dilution factor will be 10-8.
CFU/ml is given by a number of colonies/dilution factor * volume plated in ml.
Given that half, plate gives 46 colonies. So full plate will give 92 colonies.
Volume Plated = 0.1ml
Dilution factor = 10-8
92 /10-8 *0.1
92 * 108 * 10
9.2 * 1010 CFU/ml or CFU/gm of soil
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You are given a 1 gram soil sample of unknown bacterial load.
After doing 10-fold serial...
1. You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold...
1. You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies. a) What was the dilution factor? b) How many bacteria were present in the soil? 2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells. a) After 5...
In our experiment we made 10-fold serial dilutions of a sample. We made both spread plates...
In our experiment we made 10-fold
serial dilutions of a sample. We made both spread plates and pour
plates. These are our final results from last semester.
Decide which of the following statemtents are true
statements.
QUESTION 6 In our experiment we made 10-fold serial dilutions of a sample. We made both spread plates and pour plates. These are our final results from last semester. Dilution Factor Pour plates Spread Plates TMTC (300+) TMTC (300-) 10-2 TMTC (300+) 10-3 291...
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL...
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL aliquots of the dilutions are grown on agar plates and the number of colonies counted: Dilution: Number of colonies: 10- Too many to count 10-6 10- 2 0 -3 Too many 249 to count 10-4 24 2 10-5 0 BIOT 5021-SP2 Assuming that one cell gives rise to one colony, how many cells were there per mL of the original culture?
You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100,...
You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100, and 1/10. Then you plate 100 µl of the final dilution and the next day observe 200 colonies on your plate. What was the concentration of the original culture? (5 pts)
data given Procedure for Serial Dilutions: Experimental Data is given II ml 1 ml ml ml...
data given
Procedure for Serial Dilutions: Experimental Data is given II ml 1 ml ml ml I ml Tube 11 Tube 21 Tube 3 Tube 4 Il ml 1 ml ml No of bacterial Colonies TNTC TNTC 290 35 Using the Pour-Plate Method What was the purpose of this lab exercise? Complete the following table with your observations: Dilution factor Comments Number of bacteria per 1 ml of original milk Number of colonies on the plate 10 100 1,000 10,000...
I need the answer to question D, thank you. Question 10. Bacterial density in a counting...
I need the answer to question D, thank you.
Question 10. Bacterial density in a counting chamber A scientist needs to calculate the density of a bacterial culture in order to set up an experiment, but the laboratory spectrophotometer is broken and the only alternative is to use a counting chamber that she finds in a drawer of old equipment. The counting chamber has a glass to coverslip height of 0.02 mm and the instructions say that each square of...
Hello, just wanted to check my answers. Answer as many as you like/can. Thankyou. 1) Four...
Hello, just wanted to check my answers. Answer as many as you like/can. Thankyou. 1) Four grams of soil are added to 36 ml sterile water and mixed well. 0.1 ml of this mix was added to 9.9 ml sterile water. This was diluted by 4 successive 1/10 dilutions. 1.0 ml from the last dilution was used to prepare a pour plate. After incubation 289 colonies were counted. Calculate the # CFU/g soil. 2) A 0.1 ml aliquot of a...
A bacterial sample has a concentration of 2 x106 CFUlml. Show a scheme of dilutions to...
A bacterial sample has a concentration of 2 x106 CFUlml. Show a scheme of dilutions to obtain 30-300 colonies on a plate. Your scheme should contain the volume of diluent (sterile water), volume of sample transferred each time, the concentration of bacteria (CFU/mL) in each dilution. You can assume you are plating 1 mL of the dilution on plates of nutrient agar. See Figure 1 in protocol 10. Dilution factor in single transfer (change x and y to numbers 10...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
Can someone please help me with this question? I tried doing it and I got 2.4x10^8,...
Can someone please help me with this question? I tried doing it and I got 2.4x10^8, but I am not sure if that is right. Thanks in advanced. A 1/10 serial dilution of a bacterial culture was performed in tubes "A" through "E" (dilutions were made in that order beginning with tube "A" like in the handout slide titled "Spread Plating"). 100 microliters of the diluted culture from the final tube "E" was spread onto a plate. Following incubation of...
In our experiment we made 10-fold
serial dilutions of a sample. We made both spread plates and pour
plates. These are our final results from last semester.
Decide which of the following statemtents are true
statements.
QUESTION 6 In our experiment we made 10-fold serial dilutions of a sample. We made both spread plates and pour plates. These are our final results from last semester. Dilution Factor Pour plates Spread Plates TMTC (300+) TMTC (300-) 10-2 TMTC (300+) 10-3 291...
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL aliquots of the dilutions are grown on agar plates and the number of colonies counted: Dilution: Number of colonies: 10- Too many to count 10-6 10- 2 0 -3 Too many 249 to count 10-4 24 2 10-5 0 BIOT 5021-SP2 Assuming that one cell gives rise to one colony, how many cells were there per mL of the original culture?
data given
Procedure for Serial Dilutions: Experimental Data is given II ml 1 ml ml ml I ml Tube 11 Tube 21 Tube 3 Tube 4 Il ml 1 ml ml No of bacterial Colonies TNTC TNTC 290 35 Using the Pour-Plate Method What was the purpose of this lab exercise? Complete the following table with your observations: Dilution factor Comments Number of bacteria per 1 ml of original milk Number of colonies on the plate 10 100 1,000 10,000...
I need the answer to question D, thank you.
Question 10. Bacterial density in a counting chamber A scientist needs to calculate the density of a bacterial culture in order to set up an experiment, but the laboratory spectrophotometer is broken and the only alternative is to use a counting chamber that she finds in a drawer of old equipment. The counting chamber has a glass to coverslip height of 0.02 mm and the instructions say that each square of...
A bacterial sample has a concentration of 2 x106 CFUlml. Show a scheme of dilutions to obtain 30-300 colonies on a plate. Your scheme should contain the volume of diluent (sterile water), volume of sample transferred each time, the concentration of bacteria (CFU/mL) in each dilution. You can assume you are plating 1 mL of the dilution on plates of nutrient agar. See Figure 1 in protocol 10. Dilution factor in single transfer (change x and y to numbers 10...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
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