Homework Answers
Request Answer!
We need at least 10 more requests to produce the answer.
0 / 10 have requested this problem solution
The more requests, the faster the answer.
Add Answer to:
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL...
#1) What should be the concentration of kanamycin if a serial 2-fold dilution was done starting...
#1) What should be the concentration of kanamycin if a serial 2-fold dilution was done starting with tube 1 and ending in tube 9 with an initial concentration of 100 ug/mL tube # 1 2 3 4 5 6 7 8 9 10 [control] Kanamycin concentration (ug/ml) 100 ? ? ? ? ? ? ? ? 0 growth no no no no no yes yes yes yes yes #2) Culture C has a titer of 2.3 x 107 bacteria/mL. Draw...
You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial...
You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies. a) What was the dilution factor? b) How many bacteria were present in the soil?
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
Bacteria #2 is a Bacteria #3 is a III. Calculation: (1) Serial dilution: (3 pts/each) 1....
Bacteria #2 is a Bacteria #3 is a III. Calculation: (1) Serial dilution: (3 pts/each) 1. 0.2 mL of sample is added to 0.8 mL of diluent, what is the Dilution? 2. 0.01 mL of sample is added to 0.99 mL of diluent, what is the Dilution? :100 mL 0.2 mL of a sample was added to 0.8 mL of a diluent. Then, 0.1 mL of this first dilution was added to 0.4 mL of a diluent. What is the...
1. You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold...
1. You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies. a) What was the dilution factor? b) How many bacteria were present in the soil? 2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells. a) After 5...
help me with the math. m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or...
help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine...
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine viable bacterial counts. Indicate the final dilutions in each of the two tubes. Note that two different volumes of the cells intube #2 are plated onto agar plates A and B. Indicate the dilution factor that will be used to report the original cell concentration on a per mL basis. 1 ml 1 ml Final dilution in Tube #1 = Final dilution in Tube...
please help with whatever possible. thank you so much in advance. Name One use of serial...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
Write the formula for calculating the CFU/ml here and put in the numbers used. CFU (Colony...
Write the formula for calculating the CFU/ml here and put in the numbers used. CFU (Colony Forming unit) CFU/ml= (Number of Colonies|Dilution factor) (Volume of culture plate) Conclusion: In a narrative form Using the 51 (Chapter 3, Cowan lecture book) Inoculation agar, Incubation, Isolation, Inspection, Identification Use the 5 l-s to explain how it was decided that the infectious southe was or was not the cause The number of colonies in first plate is 79. The number of colonies in...
In order to count cells, the culture must be duted The technique o serialul precise and...
In order to count cells, the culture must be duted The technique o serialul precise and quantitative method alle e n al b e performed allow you to determine how many well per miller were the time the were made in overview knowvolume of culturedded to know Volume 0 sterile diluent aliquid that will not harm the cell The liquid in this tube is mixed and known volume of this culture is transferred to another known volume of sterile diluent...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
Bacteria #2 is a Bacteria #3 is a III. Calculation: (1) Serial dilution: (3 pts/each) 1. 0.2 mL of sample is added to 0.8 mL of diluent, what is the Dilution? 2. 0.01 mL of sample is added to 0.99 mL of diluent, what is the Dilution? :100 mL 0.2 mL of a sample was added to 0.8 mL of a diluent. Then, 0.1 mL of this first dilution was added to 0.4 mL of a diluent. What is the...
help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine viable bacterial counts. Indicate the final dilutions in each of the two tubes. Note that two different volumes of the cells intube #2 are plated onto agar plates A and B. Indicate the dilution factor that will be used to report the original cell concentration on a per mL basis. 1 ml 1 ml Final dilution in Tube #1 = Final dilution in Tube...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
Write the formula for calculating the CFU/ml here and put in the numbers used. CFU (Colony Forming unit) CFU/ml= (Number of Colonies|Dilution factor) (Volume of culture plate) Conclusion: In a narrative form Using the 51 (Chapter 3, Cowan lecture book) Inoculation agar, Incubation, Isolation, Inspection, Identification Use the 5 l-s to explain how it was decided that the infectious southe was or was not the cause The number of colonies in first plate is 79. The number of colonies in...
In order to count cells, the culture must be duted The technique o serialul precise and quantitative method alle e n al b e performed allow you to determine how many well per miller were the time the were made in overview knowvolume of culturedded to know Volume 0 sterile diluent aliquid that will not harm the cell The liquid in this tube is mixed and known volume of this culture is transferred to another known volume of sterile diluent...
Most questions answered within 3 hours.
-
If you’re standing at the bottom of a hill and asked to evaluate
it while being...
asked 33 minutes ago -
1. Which region has taken the lead in the world of
e-waste handling?
a) European Union...
asked 26 minutes ago -
A 8.15- g bullet from a 9-mm pistol has a velocity of 366.0 m/s.
It strikes...
asked 1 hour ago -
The outstanding bonds of Alpha Extracts have a yield to maturity
of 7.4 percent and a...
asked 1 hour ago -
The Problem: The Case of the Harmonizing Vacations
Your CEO is exploring partnering with a European...
asked 3 hours ago -
A chemical equation is balanced by adding coefficients in front
of some formulas so that the...
asked 3 hours ago -
From the literature (reference your sources): What are the
lattice parameters of calcite and aragonite? Why...
asked 3 hours ago -
Your system is rejecting the question am asking which is
preceded by a case study. It...
asked 4 hours ago -
3. On January 2, 2000, Larry creates a trust with himself as
trustee. Larry as trustee...
asked 4 hours ago -
A member of the volleyball team spikes the ball. During this
process, she changes the velocity...
asked 4 hours ago -
Are adult gamers less likely to use a gaming console (Xbox,
PlayStation, Wii, etc...) than teen...
asked 5 hours ago -
The University of
Texas recently reported that 43% of college students aged 18-24
would spend their...
asked 5 hours ago