Homework Answers
CFU per mL = # colonies × dilution factor / volume plated = # colonies / (dilution × volume plated)
# colonies = 79 + 64 / 2 = 71.5
Dilution factor = 10^4
Volume plated = 1 mL
CFU per mL = (71.5 × 10^4) / 1 = 7.15 × 10^5 = 715000
The CFU per mL is more than 500,000. So, it will be infectious.
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please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
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please help me understand step by step how to figure out these
problems.
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