What is the equation for calculating the cfu/ g soil? We used 1g soil and diluted...
What is the equation for calculating the cfu/ g soil?
We used 1g soil and diluted it in 9mL of water --> is considered to be our 10-1 dilution. --> dilution factor is 10
We plated 0.1 mL of this 10-1 solution onto an agar plate.
Can I use following equation to calculate the cfu/ g soil (density is neglected):
cfu/g soil=(no.of colonies x dilution factor)/(volume of culture plate)
cfu/g soil =( 30 cfu x 10) / 0.1 mL = 3000 cfu/ g soil
Homework Answers
Ans. CFU is the colony forming unit which we can calculate using the given formula :-
CFU/g soil= no. of colonies x dilution factor / volume of culture plated
Cfu/g = 30x 10/0.1ml= 3000cfu/g.
It is correct.
If further you have to calculate it for 5g or so, then you will have to multiply the result came for 1g with 5 or so on.
Ans. CFU is the colony forming unit which we can calculate using the given formula :-
CFU/g soil= no. of colonies x dilution factor / volume of culture plated
Cfu/g = 30x 10/0.1ml= 3000cfu/g.
It is correct.
If further you have to calculate it for 5g or so, then you will have to multiply the result came for 1g with 5 or so on.
Add Answer to:
What is the equation for calculating the cfu/ g soil?
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help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
please help me understand step by step how to figure out these
problems.
Equation 3: CFU/mL = number of colonies/mL plated TDF of the culture used for plating For example, consider the sketch below. Using the information in the figure, the plate with 50 colonies would be chosen. 1.0 mL 1.0 mL 1.0 L 1.0 mL 1.0 mL A 0.1 ml de 2.0m00m 100 m 9.0 ml 9.0 ml 9.0 mi 9.9 ml 9.0 ml Original culture 1.0 ml 2.0...
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