Plating 1.0 ml of a sample diluted to 10^-4 produced 57 colonies. What was the CFU/ml...
Plating 1.0 ml of a sample diluted to 10^-4 produced 57 colonies. What was the CFU/ml in the original sample? What would it be if you plated 0.1 ml with same colonies formed?
Homework Answers
Amount plated ---1.0 ml
Dilution----10^-4
Number of colonies in the plate--57
The CFU/ml in th eoriginal sample is---
Add Answer to:
Plating 1.0 ml of a sample diluted to 10^-4 produced 57
colonies. What was the CFU/ml...
c)You have 0.6 mL an undiluted culture at a density of 3.7 x 107 CFU/mL. You...
c)You have 0.6 mL an undiluted culture at a density of 3.7 x 107 CFU/mL. You add 5.4 mL of sterile diluent. What is the dilution and final cell density? e) Plating 2.0 mL of a sample diluted by a factor or 10-2 procedures 56 colonies. What was the original cell density in the sample? f)Plating 0.2 mL of a sample diluted by a factor or 10-4 procedures 87 colonies. What was the original cell density in the sample?
Calculate the cfu/ml using the formula cfu/ml = # colonies x dilution factor x plating factor...
Calculate the cfu/ml using the
formula
cfu/ml = # colonies x dilution factor
x plating factor (10)
Temp. (OC) Organism #Colonies Dilution Staphylococcus aureus 43 15 1:1000000 51 1:10000 1:100 53 6
9. A. You have diluted a sample by 1000 fold and plated 1 mL on an...
9. A. You have diluted a sample by 1000 fold and plated 1 mL on an agar plate. You observe 35 colonies. What was the concentration of the original sample in CFU/mL?
please help me understand step by step how to figure out these problems. Equation 3: CFU/mL...
please help me understand step by step how to figure out these
problems.
Equation 3: CFU/mL = number of colonies/mL plated TDF of the culture used for plating For example, consider the sketch below. Using the information in the figure, the plate with 50 colonies would be chosen. 1.0 mL 1.0 mL 1.0 L 1.0 mL 1.0 mL A 0.1 ml de 2.0m00m 100 m 9.0 ml 9.0 ml 9.0 mi 9.9 ml 9.0 ml Original culture 1.0 ml 2.0...
how to solve 9-10 9. After plating 0.1ml of a 10 dilution, 158 colonies grow. What...
how to solve 9-10
9. After plating 0.1ml of a 10 dilution, 158 colonies grow. What is the OCD? (CFU/ml) 158 3,514 (105) = 1.58×10 CFU/mL a account for Sci not. dec. 10. After plating 0.2 ml of a 10 tube dilution, 437 colonies grow. What is the CFU/ml or how would you report this info? 437 0/2 OCD 2.16x100 CP /ML 0.2110-5)
What is the equation for calculating the cfu/ g soil? We used 1g soil and diluted...
What is the equation for calculating the cfu/ g soil? We used 1g soil and diluted it in 9mL of water --> is considered to be our 10-1 dilution. --> dilution factor is 10 We plated 0.1 mL of this 10-1 solution onto an agar plate. Can I use following equation to calculate the cfu/ g soil (density is neglected): cfu/g soil=(no.of colonies x dilution factor)/(volume of culture plate) cfu/g soil =( 30 cfu x 10) / 0.1 mL =...
What is the original concentration of cells in a sample that produced 218 colonies after spread...
What is the original concentration of cells in a sample that produced 218 colonies after spread - plating 0.1 ml from a tube with a 5/100000 overall dilution factor?
help me with the math. m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or...
help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
your lab manual for instructions, Sample plated Number of colonies on plate TMTC 430 4th plate...
your lab manual for instructions, Sample plated Number of colonies on plate TMTC 430 4th plate 5th plate 6th plate 75 Which plate has the countable number of colonies? What is the dilution factor that produced that plate? • What is the correction factor for the volume? How many cfu/ml are in the culture, based on your calculations? 4. Suppose you plated 1 ml of a 10-7 dilution (tenfold dilutions) of a bacterial broth culture
Answer and do the following calculations: 1. Suppose your professor handed you a test tube with...
Answer and do the following calculations: 1. Suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10–2 dilution of the entire culture. Explain how you would do this. Show your calculations. 2. How would you produce a 10–1 dilution of a 3 mL bacterial sample using the entire 3 mL volume? 3. You have 0.05 mL of an undiluted culture at a concentration of...
Calculate the cfu/ml using the
formula
cfu/ml = # colonies x dilution factor
x plating factor (10)
Temp. (OC) Organism #Colonies Dilution Staphylococcus aureus 43 15 1:1000000 51 1:10000 1:100 53 6
please help me understand step by step how to figure out these
problems.
Equation 3: CFU/mL = number of colonies/mL plated TDF of the culture used for plating For example, consider the sketch below. Using the information in the figure, the plate with 50 colonies would be chosen. 1.0 mL 1.0 mL 1.0 L 1.0 mL 1.0 mL A 0.1 ml de 2.0m00m 100 m 9.0 ml 9.0 ml 9.0 mi 9.9 ml 9.0 ml Original culture 1.0 ml 2.0...
how to solve 9-10
9. After plating 0.1ml of a 10 dilution, 158 colonies grow. What is the OCD? (CFU/ml) 158 3,514 (105) = 1.58×10 CFU/mL a account for Sci not. dec. 10. After plating 0.2 ml of a 10 tube dilution, 437 colonies grow. What is the CFU/ml or how would you report this info? 437 0/2 OCD 2.16x100 CP /ML 0.2110-5)
help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
your lab manual for instructions, Sample plated Number of colonies on plate TMTC 430 4th plate 5th plate 6th plate 75 Which plate has the countable number of colonies? What is the dilution factor that produced that plate? • What is the correction factor for the volume? How many cfu/ml are in the culture, based on your calculations? 4. Suppose you plated 1 ml of a 10-7 dilution (tenfold dilutions) of a bacterial broth culture
Most questions answered within 3 hours.
-
A 8.15- g bullet from a 9-mm pistol has a velocity of 366.0 m/s.
It strikes...
asked 33 minutes ago -
The outstanding bonds of Alpha Extracts have a yield to maturity
of 7.4 percent and a...
asked 30 minutes ago -
The Problem: The Case of the Harmonizing Vacations
Your CEO is exploring partnering with a European...
asked 1 hour ago -
A chemical equation is balanced by adding coefficients in front
of some formulas so that the...
asked 1 hour ago -
From the literature (reference your sources): What are the
lattice parameters of calcite and aragonite? Why...
asked 2 hours ago -
Your system is rejecting the question am asking which is
preceded by a case study. It...
asked 2 hours ago -
3. On January 2, 2000, Larry creates a trust with himself as
trustee. Larry as trustee...
asked 2 hours ago -
A member of the volleyball team spikes the ball. During this
process, she changes the velocity...
asked 2 hours ago -
Are adult gamers less likely to use a gaming console (Xbox,
PlayStation, Wii, etc...) than teen...
asked 3 hours ago -
The University of
Texas recently reported that 43% of college students aged 18-24
would spend their...
asked 3 hours ago -
The length of stay at a specific emergency department in
Phoenix, Arizona, in 2009 had a...
asked 3 hours ago -
. Please give the mechanism for this type of problem. Step by
Step
The toxin that...
asked 3 hours ago