What is the original concentration of cells in a sample that produced 218 colonies after spread...
What is the original concentration of cells in a sample that produced 218 colonies after spread - plating 0.1 ml from a tube with a 5/100000 overall dilution factor?
Homework Answers
Please look in the image for solution:
Here dilution factor should be 5×10^5 because the original concentration have higher bacterial count as compared to the volume plated.
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What is the original concentration of cells in a sample that
produced 218 colonies after spread...
3. A serial dilution of a swab sample was prepared by adding 10 uL to a...
3. A serial dilution of a swab sample was prepared by adding 10 uL to a volume of 990 ML water in tube 1, and 10 uL to a volume of 990 uL water in tube 2. a. What is the overall dilution and dilution factor of each tube? Overall Dilution Dilution Factor Tube 1 Tube 2 b. If 100 uL of tube 2 is spread plated and 126 colonies grow, what was the #cells/mL in the original sample?
If you obtain 35 colonies after spread plating 1 ml of a 1:500 dilution of lake...
If you obtain 35 colonies after spread plating 1 ml of a 1:500 dilution of lake water, what is the concentration of bacteria in the lake water?
Plating 1.0 ml of a sample diluted to 10^-4 produced 57 colonies. What was the CFU/ml...
Plating 1.0 ml of a sample diluted to 10^-4 produced 57 colonies. What was the CFU/ml in the original sample? What would it be if you plated 0.1 ml with same colonies formed?
You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24...
You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? (2 points) B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8)...
Microbiology In tube #1, the original concentration of penicillin is 1000 units per ml. In a...
Microbiology
In tube #1, the original concentration of penicillin is 1000 units per ml. In a 1/5 dilution series, the first tube to show growth is tube #7. What is the m nmal hibitory concentration of penicillin in this assay? Given a plate with 58 colonies, determine the original concentration of bacteria in tube #1 for each of out 1.0 ml fontube #4 of a 1/10 dilution series, c) if one has plated out 0.1 ml from tube #5 of...
how to solve 9-10 9. After plating 0.1ml of a 10 dilution, 158 colonies grow. What...
how to solve 9-10
9. After plating 0.1ml of a 10 dilution, 158 colonies grow. What is the OCD? (CFU/ml) 158 3,514 (105) = 1.58×10 CFU/mL a account for Sci not. dec. 10. After plating 0.2 ml of a 10 tube dilution, 437 colonies grow. What is the CFU/ml or how would you report this info? 437 0/2 OCD 2.16x100 CP /ML 0.2110-5)
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates,...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates, resulting in the growth of an average of 68 colonies per plate. What is the concentration of colony-forming units (cfu/mi) present in the original culture? 0.1 ml 0.1 ml 1 ml 1 ml 100 ml 9.9 ml 9.9 ml 9 ml 9 ml 2 4 3. The culture flask at left contains a total of 3.2 x 10s cfu....
A bacterial sample has a concentration of 2 x106 CFUlml. Show a scheme of dilutions to...
A bacterial sample has a concentration of 2 x106 CFUlml. Show a scheme of dilutions to obtain 30-300 colonies on a plate. Your scheme should contain the volume of diluent (sterile water), volume of sample transferred each time, the concentration of bacteria (CFU/mL) in each dilution. You can assume you are plating 1 mL of the dilution on plates of nutrient agar. See Figure 1 in protocol 10. Dilution factor in single transfer (change x and y to numbers 10...
please help with whatever possible. thank you so much in advance. Name One use of serial...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
3. A serial dilution of a swab sample was prepared by adding 10 uL to a volume of 990 ML water in tube 1, and 10 uL to a volume of 990 uL water in tube 2. a. What is the overall dilution and dilution factor of each tube? Overall Dilution Dilution Factor Tube 1 Tube 2 b. If 100 uL of tube 2 is spread plated and 126 colonies grow, what was the #cells/mL in the original sample?
Microbiology
In tube #1, the original concentration of penicillin is 1000 units per ml. In a 1/5 dilution series, the first tube to show growth is tube #7. What is the m nmal hibitory concentration of penicillin in this assay? Given a plate with 58 colonies, determine the original concentration of bacteria in tube #1 for each of out 1.0 ml fontube #4 of a 1/10 dilution series, c) if one has plated out 0.1 ml from tube #5 of...
how to solve 9-10
9. After plating 0.1ml of a 10 dilution, 158 colonies grow. What is the OCD? (CFU/ml) 158 3,514 (105) = 1.58×10 CFU/mL a account for Sci not. dec. 10. After plating 0.2 ml of a 10 tube dilution, 437 colonies grow. What is the CFU/ml or how would you report this info? 437 0/2 OCD 2.16x100 CP /ML 0.2110-5)
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates, resulting in the growth of an average of 68 colonies per plate. What is the concentration of colony-forming units (cfu/mi) present in the original culture? 0.1 ml 0.1 ml 1 ml 1 ml 100 ml 9.9 ml 9.9 ml 9 ml 9 ml 2 4 3. The culture flask at left contains a total of 3.2 x 10s cfu....
A bacterial sample has a concentration of 2 x106 CFUlml. Show a scheme of dilutions to obtain 30-300 colonies on a plate. Your scheme should contain the volume of diluent (sterile water), volume of sample transferred each time, the concentration of bacteria (CFU/mL) in each dilution. You can assume you are plating 1 mL of the dilution on plates of nutrient agar. See Figure 1 in protocol 10. Dilution factor in single transfer (change x and y to numbers 10...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
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