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3. A serial dilution of a swab sample was prepared by adding 10 uL to a...
900 ul diluent 50 ul 450 ul diluent 10 ul 990 ul diluent 100 ul cells...
900 ul diluent 50 ul 450 ul diluent 10 ul 990 ul diluent 100 ul cells Plate A 323 cfu 100 ul plated Plate B 10 ul plated QUESTION 6: a) The original concentration of the 100 ul cell suspension is b) The final dilution factor on Plate A is c) You would expect to count colonies on Plate B.
After making a serial dilution a tank water sample, you plate 100 ul of the 10-4...
After making a serial dilution a tank water sample, you plate 100 ul of the 10-4 After incubating, you count 90 colonies on the plate. What is the CFU/ml? Write out your calculations, briefly, along with your final answer.
1. Calculate the D.F for a bacterial culture if 2000uL of undiluted cuiture was added to a nutrie...
#1-6
1. Calculate the D.F for a bacterial culture if 2000uL of undiluted cuiture was added to a nutrient broth to bring up the final volume to 1 liter. (2 pts) a. 102 b. 2 X 10 c. 2 X 104 2. It you plated 20 ul of sample with a dilution factor of 10-3, what would your total sample volume be? (Total sample volume - (Volume plated) x (Dilution)). (2 pts) 3. Calculate the O.C.D using a viable count...
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine...
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine viable bacterial counts. Indicate the final dilutions in each of the two tubes. Note that two different volumes of the cells intube #2 are plated onto agar plates A and B. Indicate the dilution factor that will be used to report the original cell concentration on a per mL basis. 1 ml 1 ml Final dilution in Tube #1 = Final dilution in Tube...
please help with whatever possible. thank you so much in advance. Name One use of serial...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
Please show how to do these enumeration questions by showing the work. What is the dilution...
Please show how to do these enumeration questions by showing the work. What is the dilution factor if you add 625 µL of culture to 4.375 mL of dilution medium? You count the 10-3 dilution of a yeast suspension using the 1/25 mm2 boxes : you find 45 cells in box 1, 15 cells in box 2, 42 cells in box 3, 23 cells in box 4, and 26 cells in box 5. a) What is the original concentration of...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
Answer the following questions regarding a 10x Serial Dilution experiment using yeast cell cultures from tube...
Answer the following questions regarding a 10x Serial Dilution experiment using yeast cell cultures from tube A. Tube A contains 1 ml of yeast culture. 3.6 ml of sterile water is placed into 4 tubes (B, C, D, and E). 0.4 ml of yeast culture is removed from tube A and put into tube B.. 0.4 ml is transferred from tube B to tube C, and from tube C to tube E. 1.) What is the final volume in tubes...
What is the original concentration of cells in a sample that produced 218 colonies after spread...
What is the original concentration of cells in a sample that produced 218 colonies after spread - plating 0.1 ml from a tube with a 5/100000 overall dilution factor?
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL...
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL aliquots of the dilutions are grown on agar plates and the number of colonies counted: Dilution: Number of colonies: 10- Too many to count 10-6 10- 2 0 -3 Too many 249 to count 10-4 24 2 10-5 0 BIOT 5021-SP2 Assuming that one cell gives rise to one colony, how many cells were there per mL of the original culture?
900 ul diluent 50 ul 450 ul diluent 10 ul 990 ul diluent 100 ul cells Plate A 323 cfu 100 ul plated Plate B 10 ul plated QUESTION 6: a) The original concentration of the 100 ul cell suspension is b) The final dilution factor on Plate A is c) You would expect to count colonies on Plate B.
After making a serial dilution a tank water sample, you plate 100 ul of the 10-4 After incubating, you count 90 colonies on the plate. What is the CFU/ml? Write out your calculations, briefly, along with your final answer.
#1-6
1. Calculate the D.F for a bacterial culture if 2000uL of undiluted cuiture was added to a nutrient broth to bring up the final volume to 1 liter. (2 pts) a. 102 b. 2 X 10 c. 2 X 104 2. It you plated 20 ul of sample with a dilution factor of 10-3, what would your total sample volume be? (Total sample volume - (Volume plated) x (Dilution)). (2 pts) 3. Calculate the O.C.D using a viable count...
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine viable bacterial counts. Indicate the final dilutions in each of the two tubes. Note that two different volumes of the cells intube #2 are plated onto agar plates A and B. Indicate the dilution factor that will be used to report the original cell concentration on a per mL basis. 1 ml 1 ml Final dilution in Tube #1 = Final dilution in Tube...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL aliquots of the dilutions are grown on agar plates and the number of colonies counted: Dilution: Number of colonies: 10- Too many to count 10-6 10- 2 0 -3 Too many 249 to count 10-4 24 2 10-5 0 BIOT 5021-SP2 Assuming that one cell gives rise to one colony, how many cells were there per mL of the original culture?
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