Homework Answers
a. Dilution factor = 10^-4 (See B)
Final cell count on plate A = 323 CFU/100 uL
= 3230 CFU/mL
b. 100 uL into 900 uL ----> 1:10 dilution
50 uL into 450 uL ----> 1:10 dilution
10 uL into 990 uL ----> 1:100 dilution
Total dilution factor = 1:10000 = 10^-4
c. 100 uL ------> 323 CFU
10 uL = 32 CFU
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please help with whatever possible. thank you so much in
advance.
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#1-6
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please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
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#1-6
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