If you started with adding 50 ul of an overnight culture to 500 mls of LB...

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If you started with adding 50 ul of an overnight culture to 500 mls of LB...

If you started with adding 50 ul of an overnight culture to 500 mls of LB media and grew the culture at 37oC for 4 hours and plated 200 ul of a cell suspension and observed 100 colonies on your plate, how many cells were in the original overnight culture used to inoculate the LB media?

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Answer 1

Answer #1

Answer:

1) 50 microlitre culture was added to .......... 500 mls of LB Media.............. culture grown at 37 degree for 4 hours

2) Plated on petriplate of about 200 microlitres

3) Observed 100 colonies

= 100 (colonies)/ 0.2 ml or 200 microlitres

= 500 CFU/litre

so, there are 500 cfu per litre.

As 50 microlitre is added to 500 ml of LB broth...it is 10⁴ times diluted so..

500 cfu x 10⁴ = 5 x 10⁶ cells or cfu per litre in the overnight culture used to inoculate.

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Answer 2

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