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1. Calculate the D.F for a bacterial culture if 2000uL of undiluted cuiture was added to a nutrie...
A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water....
A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution? b) How many cfu/mL were present in the original culture? c) How many...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
3. A serial dilution of a swab sample was prepared by adding 10 uL to a...
3. A serial dilution of a swab sample was prepared by adding 10 uL to a volume of 990 ML water in tube 1, and 10 uL to a volume of 990 uL water in tube 2. a. What is the overall dilution and dilution factor of each tube? Overall Dilution Dilution Factor Tube 1 Tube 2 b. If 100 uL of tube 2 is spread plated and 126 colonies grow, what was the #cells/mL in the original sample?
Answer and do the following calculations: 1. Suppose your professor handed you a test tube with...
Answer and do the following calculations: 1. Suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10–2 dilution of the entire culture. Explain how you would do this. Show your calculations. 2. How would you produce a 10–1 dilution of a 3 mL bacterial sample using the entire 3 mL volume? 3. You have 0.05 mL of an undiluted culture at a concentration of...
Question 31 15 pts A bacterial sample is tested for its concentration in a plate account...
Question 31 15 pts A bacterial sample is tested for its concentration in a plate account assay. This sample is diluted in a 1:10 dilution series. One tenth of milliliter of the tube of a dilution factor (104) was plated in a pour plate. After incubation, the plate has 31 colonies. Calculate the concentration of bacterial cells in this sample.
What is the concentration of cells in the starting sample if 1 ml was plated per...
What is the concentration of cells in the starting sample if 1
ml was plated per dilution and the starting sample was river
water?
Report your answer below as a decimal with one significant digit
after the decimal and report the exponent the separate box. See
this example, if your answer is 5.2 x 108 you would
report it as "5.2"
You have the following results from a serial dilution experiment: Sample Dilution Plate Count 1:101 Too many to count...
please help with #3 and #4 3. Did you enumerate all the bact u you enumerate...
please help with #3 and #4
3. Did you enumerate all the bact u you enumerate all the bacteria present in the sample when you used the standard plate count method? Explain. 4. If you add 1.0 ml to 99 ml of water, what is the dilution of the sample? Results What is the number of colonies in each plate? 10-5 Culture 10-3 dilution plate 104 10-6 dilution plate dilution plate dilution plate Too numerous to 1848 - 384 1247...
Meat continued: Plate A Plate B Plate C Gr.Beel, 3 da. 4C, 10-4 dilution Gr.Beel, 3...
Meat continued: Plate A Plate B Plate C Gr.Beel, 3 da. 4C, 10-4 dilution Gr.Beel, 3 da. @ 4C, 10-2 dilution Gr.Beef for 3 da. @ 4C, 10-3 dilution Ground meat was stomached after addition of peptone water. The solution was diluted 10x (10-1), 100x (10-2), 1000x (10-3), 10.000x (10-4). Using the three latter dilutions, 100 ul was spread plated on to TSA and incubated at 37°C. Show dilution series and indicate which dilutions were plated: To estimate cell concentration...
Suppose you would like to determine the number of bacteria in a culture via an aerobic...
Suppose you would like to determine the number of bacteria in a culture via an aerobic plate count. Explain (with pictures) how you would set up a dilution series (104, 106, 10) using the fewest number of dilution blanks. Draw and label your tubes and plates with each dilution. Show all volumes including transfers, amounts in tubes, and amounts plated 1. You want to set up a 1:10 dilution series, so that you can plate a 10-1, 102, and a...
questions 6 and 7 please with work 6. (2 points) You dilute an unknown sample of...
questions 6 and 7 please with work
6. (2 points) You dilute an unknown sample of bacteria to 1x 10- and plate out 10ul onto a Petri dish. The next day you count 64 colonies. The number of viable bacteria in the original sample was a. 6.4 x 105 cells/ul b. 6.4 x 106 cells/ul, Answer: C. 6.4 x 10' cells/ul d. 6.4 x 108 cells/ul 7. (2 points) A sample of bacteria was diluted to 1x 10 and 10ul...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
3. A serial dilution of a swab sample was prepared by adding 10 uL to a volume of 990 ML water in tube 1, and 10 uL to a volume of 990 uL water in tube 2. a. What is the overall dilution and dilution factor of each tube? Overall Dilution Dilution Factor Tube 1 Tube 2 b. If 100 uL of tube 2 is spread plated and 126 colonies grow, what was the #cells/mL in the original sample?
Question 31 15 pts A bacterial sample is tested for its concentration in a plate account assay. This sample is diluted in a 1:10 dilution series. One tenth of milliliter of the tube of a dilution factor (104) was plated in a pour plate. After incubation, the plate has 31 colonies. Calculate the concentration of bacterial cells in this sample.
What is the concentration of cells in the starting sample if 1
ml was plated per dilution and the starting sample was river
water?
Report your answer below as a decimal with one significant digit
after the decimal and report the exponent the separate box. See
this example, if your answer is 5.2 x 108 you would
report it as "5.2"
You have the following results from a serial dilution experiment: Sample Dilution Plate Count 1:101 Too many to count...
please help with #3 and #4
3. Did you enumerate all the bact u you enumerate all the bacteria present in the sample when you used the standard plate count method? Explain. 4. If you add 1.0 ml to 99 ml of water, what is the dilution of the sample? Results What is the number of colonies in each plate? 10-5 Culture 10-3 dilution plate 104 10-6 dilution plate dilution plate dilution plate Too numerous to 1848 - 384 1247...
Meat continued: Plate A Plate B Plate C Gr.Beel, 3 da. 4C, 10-4 dilution Gr.Beel, 3 da. @ 4C, 10-2 dilution Gr.Beef for 3 da. @ 4C, 10-3 dilution Ground meat was stomached after addition of peptone water. The solution was diluted 10x (10-1), 100x (10-2), 1000x (10-3), 10.000x (10-4). Using the three latter dilutions, 100 ul was spread plated on to TSA and incubated at 37°C. Show dilution series and indicate which dilutions were plated: To estimate cell concentration...
Suppose you would like to determine the number of bacteria in a culture via an aerobic plate count. Explain (with pictures) how you would set up a dilution series (104, 106, 10) using the fewest number of dilution blanks. Draw and label your tubes and plates with each dilution. Show all volumes including transfers, amounts in tubes, and amounts plated 1. You want to set up a 1:10 dilution series, so that you can plate a 10-1, 102, and a...
questions 6 and 7 please with work
6. (2 points) You dilute an unknown sample of bacteria to 1x 10- and plate out 10ul onto a Petri dish. The next day you count 64 colonies. The number of viable bacteria in the original sample was a. 6.4 x 105 cells/ul b. 6.4 x 106 cells/ul, Answer: C. 6.4 x 10' cells/ul d. 6.4 x 108 cells/ul 7. (2 points) A sample of bacteria was diluted to 1x 10 and 10ul...
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