ZuoTi.Pro
Question

6. (2 points) You dilute an unknown sample of bacteria to 1x 10- and plate out 10ul onto a Petri dish. The next day you count questions 6 and 7 please with work
science   biology   
0 0
Add a comment Improve this question Transcribed image text
Next > < Previous
Sort answers by oldest

Homework Answers

Answer #1

6. Option b is correct : The number of viable bacteria in the original sample is 6.4x10^6 cells/microlitre.

7. Approximate concentration of the original sample was 1.38x10^4 cells/microlitre or 1.38x10^7 cells/ml.

The required calculation is given in the below image6. Unknown sample of bacteria is diluted to 1x10-6 .. Dilution factors = 106 And froom that coul is plated out onto a pitorid

0 0
Add a comment
Know the answer?
Add Answer to:
questions 6 and 7 please with work 6. (2 points) You dilute an unknown sample of...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions

  • I'm not sure if I completed question 3 correctly. Also, I need help determing question 4....

    I'm not sure if I completed question 3 correctly. Also, I need help determing question 4. Y-0.301 6.51-hardts.al 3. A solution containing 100 mL of bacterial broth is subcultured onto 5 plates using the pour-plate method. Each plate receives broth according to the following Table. The Table also lists the results colony counts after 24 hours of incubation. Calculate the number of bacteria per milliliter of the bacterial broth for each of the dilutions. Plate Volume Broth Colonies 331 2...

  • 1. Calculate the D.F for a bacterial culture if 2000uL of undiluted cuiture was added to a nutrie...

    #1-6 1. Calculate the D.F for a bacterial culture if 2000uL of undiluted cuiture was added to a nutrient broth to bring up the final volume to 1 liter. (2 pts) a. 102 b. 2 X 10 c. 2 X 104 2. It you plated 20 ul of sample with a dilution factor of 10-3, what would your total sample volume be? (Total sample volume - (Volume plated) x (Dilution)). (2 pts) 3. Calculate the O.C.D using a viable count...

  • I need help woth Q5 and Q6 please. I am not sure of my answers. 5....

    I need help woth Q5 and Q6 please. I am not sure of my answers. 5. Two curious scientists, Gregor and Barbara, were studying E.coli infected with a virulent bacteriophage. They noticed that most of the bacteria were lysed, but a few survived. Gregor hypothesized that the bacteriophage induced the resistance in the cells, while Barbara hypothesized that the resistant mutants probably already existed. To test this, they seeded E. coli onto a petri dish which grew 10 colonies, and...

  • A. You have been given a tube of E. coli. You are asked to make 1...

    A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells   +    _____ ml water    = 1 ml    (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...

  • You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24...

    You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? (2 points) B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8)...

  • Please show how to do these enumeration questions by showing the work. What is the dilution...

    Please show how to do these enumeration questions by showing the work. What is the dilution factor if you add 625 µL of culture to 4.375 mL of dilution medium? You count the 10-3 dilution of a yeast suspension using the 1/25 mm2 boxes : you find 45 cells in box 1, 15 cells in box 2, 42 cells in box 3, 23 cells in box 4, and 26 cells in box 5. a) What is the original concentration of...

  • please help with whatever possible. thank you so much in advance. Name One use of serial...

    please help with whatever possible. thank you so much in advance. Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...

  • 1. You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold...

    1. You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies.   a) What was the dilution factor? b) How many bacteria were present in the soil? 2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells.    a) After 5...

  • Hello, just wanted to check my answers. Answer as many as you like/can. Thankyou. 1) Four...

    Hello, just wanted to check my answers. Answer as many as you like/can. Thankyou. 1) Four grams of soil are added to 36 ml sterile water and mixed well. 0.1 ml of this mix was added to 9.9 ml sterile water. This was diluted by 4 successive 1/10 dilutions. 1.0 ml from the last dilution was used to prepare a pour plate. After incubation 289 colonies were counted. Calculate the # CFU/g soil. 2) A 0.1 ml aliquot of a...

  • please help with ALL questions thank you 1. Using excel, plot your standard curve based on...

    please help with ALL questions thank you 1. Using excel, plot your standard curve based on Table 9-1 and calculate the line of best fit. Put concentration in the x column and OD in the y to solve for the concentrations of your unknown in Table 9-3. Report the slope and the y-intercept below. (1 mark) Slope (m) = v-intercept(b) = 2. Can this standard curve for yeast cells be used for cali cells (why/ why not?) (2 marks) 3....

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT