Question

5. Two curious scientists, Gregor and Barbara, were studying E.coli infected with a virulent bacteriophage. They noticed that most of the bacteria were lysed, but a few survived. Gregor hypothesized that the bacteriophage induced the resistance in the cells, while Barbara hypothesized that the resistant mutants probably already existed. To test this, they seeded E. coli onto a petri dish which grew 10 colonies, and replica plated this master plate. They pipette a suspension of the bacteriophage onto each of the three replica plates. What should they see if Gregor is correct? What should they see if Barbara is correct? (Barbara . The resistant colonies will grow in the (same position onall three plates The resistant colonies will grow and will look different from onginal plate and position 6. Circle all that are correct: Intron/Exon splicing occurs in a. Nucleus b. Cytoplasm Ribosomes d. Spliceosomes Eukaryotes f. Prokaryotes

I need help woth Q5 and Q6 please. I am not sure of my answers.

Answer 1

5) according to Gregor the bacteriophage induced resistance in the bacteria, then after replica plating the bacterial colonies to plates containing bacteriophage, different colonies will grow on different plates, that is those colonies which get mutation in the presence of bacteriophage will be different and three different plates with bacteriophage will have different pattern of colonies.

according to Barbara, the mutations were already there in the bacteria, then after replica plating, the bacterial colonies that grow in the presence of the bacteriophage will be the same. ( mutations were already present so the pattern ( position of live colonies) in all the three plates with bacteriophage will be the same)

6) splicing is the process by which introns are removed and exons are joined together. splicing of the pre-mRNA occurs in the nucleus of the eukaryotes, and the spliceosomes catalyze this reaction.

prokaryotes do not have introns so the mRNA in the prokaryotes do not undergo splicing.

so the answers area) nucleus e) eukaryotes

( the question is intron-exon splicing occurs in ....., the spliceosome catalyze splicing but splicing does not occur in spliceosome)