Question

Meat continued: Plate A Plate B Plate C Gr.Beel, 3 da. 4C, 10-4 dilution Gr.Beel, 3 da. @ 4C, 10-2 dilution Gr.Beef for 3 da. @ 4C, 10-3 dilution Ground meat was stomached after addition of peptone water. The solution was diluted 10x (10-1), 100x (10-2), 1000x (10-3), 10.000x (10-4). Using the three latter dilutions, 100 ul was spread plated on to TSA and incubated at 37°C. Show dilution series and indicate which dilutions were plated: To estimate cell concentration (CFU/ml) in the undiluted tube of stomached meat, plate B was selected, because it contains between 30 and 300 colonies. Show calculation here (count only the large colonies):

Answer 1

Resolution of the picture of  plate B is not so good so counting colonies accurately is difficult so I have roughly estimated number of colonies and then solved the problem ?.You can replace the assumed number of colonies with the accurate number by counting yourself from the actual plate and rest is same ?Good luck