Question

1. The highly pathogenic bacterium Clostridium perfringens causes gangrene, a disease that results in the destruction of animal tissue. The bacterium secretes two types of collagenases that cleave the peptide bonds linking Gly and Pro residues. A biochemical company sells a partially purified preparation of these collagenases to investigators who are interested in culturing cells deived from bone, cartilate, muscle, or endothelial tissue. How does this enzyme preparation assist the investigator in obtaining cells for culturing?

2. An enzyme found in tadpoles cleaves the peptide bond indicated in the figure below. What protein might contain this sequence and what is the role of this cleavage in the metamorphosis of the tadpole to the adult frog?

Gly-Pro-Gln-Ile-Ala

Answer 1

Cell culturing describe the method to grow the cells at the laboratory. It needs enzymes that dissociate the tissues , so that they release their cell , from which they are made. Collegenase enzyme break that peptide bonds in collagen protein . This protein is present in the tissue like skin, bones, blood, cartilage and others. So, to break this tissue, we need, collegenase enzyme and to get the cells from this tissue. The colleganase enzyme break the peptide bond by breaking glycine and Proline bond.

The enzyme that is found in the tadpole that cleaves peptide bond indicated in the figure is collegenase. This enzyme is used to break the collagen bond. This protein serve to break the structural integrity of the tail of the tadpoles.