Homework Answers
3. The sample maybe having both living and dead bacteria. So only the living bacteria was counted using the plate count method but dead bacteria which was also present in the sample was not counted using this method. This is because that bacteria will not at all grow in any nutrient media plate.
4. Dilution = initial volume / final volume = 1 / 1 + 99 = 1/100
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please help with #3 and #4
3. Did you enumerate all the bact u you enumerate...
3. You are measuring the growth of bacterium using cfu mL·You collect samples at various times an...
3. You are measuring the growth of bacterium using cfu mL·You collect samples at various times and dilute them in 10-fold series (as shown in the 2 row of the table) and plate 0.1 ml of each of these dilutions on five standard lab agar plates. The average number of colonies per plate are below Time of Colonies on plate at each dilution ars 45 290 TNTC TNTC 195 20 TNTC TNTC 200 TNTC too numerous to count ADB MO...
3. You are measuring the growth of bacterium using cfu mL·You collect samples at various times...
3. You are measuring the growth of bacterium using cfu mL·You collect samples at various times and dilute them in 10-fold series (as shown in the 2 row of the table) and plate 0.1 ml of each of these dilutions on five standard lab agar plates. The average number of colonies per plate are below Time of Colonies on plate at each dilution ars 45 290 TNTC TNTC 195 20 TNTC TNTC 200 TNTC too numerous to count ADB MO...
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
Please show how to do these enumeration questions by showing the work. What is the dilution...
Please show how to do these enumeration questions by showing the work. What is the dilution factor if you add 625 µL of culture to 4.375 mL of dilution medium? You count the 10-3 dilution of a yeast suspension using the 1/25 mm2 boxes : you find 45 cells in box 1, 15 cells in box 2, 42 cells in box 3, 23 cells in box 4, and 26 cells in box 5. a) What is the original concentration of...
please help with whatever possible. thank you so much in advance. Name One use of serial...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
Meat continued: Plate A Plate B Plate C Gr.Beel, 3 da. 4C, 10-4 dilution Gr.Beel, 3...
Meat continued: Plate A Plate B Plate C Gr.Beel, 3 da. 4C, 10-4 dilution Gr.Beel, 3 da. @ 4C, 10-2 dilution Gr.Beef for 3 da. @ 4C, 10-3 dilution Ground meat was stomached after addition of peptone water. The solution was diluted 10x (10-1), 100x (10-2), 1000x (10-3), 10.000x (10-4). Using the three latter dilutions, 100 ul was spread plated on to TSA and incubated at 37°C. Show dilution series and indicate which dilutions were plated: To estimate cell concentration...
please help me understand step by step how to figure out these problems. Equation 3: CFU/mL...
please help me understand step by step how to figure out these
problems.
Equation 3: CFU/mL = number of colonies/mL plated TDF of the culture used for plating For example, consider the sketch below. Using the information in the figure, the plate with 50 colonies would be chosen. 1.0 mL 1.0 mL 1.0 L 1.0 mL 1.0 mL A 0.1 ml de 2.0m00m 100 m 9.0 ml 9.0 ml 9.0 mi 9.9 ml 9.0 ml Original culture 1.0 ml 2.0...
Count the number of CFU from each of the plates (record TNTC if there are too...
Count the number of CFU from each of the plates (record TNTC if there are too many to count). Record your counts below and calculate the total number of bacteria per mL and the number of ampicillin-resistant bacteria per mL in the chicken fluid. Show all your calculations in a neat logical manner in the workspace on page 4. Plate Count Plates Without Ampicillin Dilution CFU/Plate Plate # 1 Plate # 2 Average/Plate CFU/mL of Fluid 10-4 52 80 66...
4. You count 97 colonies on a plate inoculated with 2.5 ml of a 10* dilution...
4. You count 97 colonies on a plate inoculated with 2.5 ml of a 10* dilution of bacteria. How many CFU per ml of bacteria were in the uridiluted stock? (1 point)
4. From a culture of M. luteus, you take 10 L and dilute it into 9990uL...
4. From a culture of M. luteus, you take 10 L and dilute it into 9990uL of sterile water. From this dilution, you take an aliquot of 10uL and plate it onto fresh agar. The following day, you observe 96 colonies on the plate. What is the original CFU/mL of your culture of M. luteus?
3. You are measuring the growth of bacterium using cfu mL·You collect samples at various times and dilute them in 10-fold series (as shown in the 2 row of the table) and plate 0.1 ml of each of these dilutions on five standard lab agar plates. The average number of colonies per plate are below Time of Colonies on plate at each dilution ars 45 290 TNTC TNTC 195 20 TNTC TNTC 200 TNTC too numerous to count ADB MO...
3. You are measuring the growth of bacterium using cfu mL·You collect samples at various times and dilute them in 10-fold series (as shown in the 2 row of the table) and plate 0.1 ml of each of these dilutions on five standard lab agar plates. The average number of colonies per plate are below Time of Colonies on plate at each dilution ars 45 290 TNTC TNTC 195 20 TNTC TNTC 200 TNTC too numerous to count ADB MO...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
Meat continued: Plate A Plate B Plate C Gr.Beel, 3 da. 4C, 10-4 dilution Gr.Beel, 3 da. @ 4C, 10-2 dilution Gr.Beef for 3 da. @ 4C, 10-3 dilution Ground meat was stomached after addition of peptone water. The solution was diluted 10x (10-1), 100x (10-2), 1000x (10-3), 10.000x (10-4). Using the three latter dilutions, 100 ul was spread plated on to TSA and incubated at 37°C. Show dilution series and indicate which dilutions were plated: To estimate cell concentration...
please help me understand step by step how to figure out these
problems.
Equation 3: CFU/mL = number of colonies/mL plated TDF of the culture used for plating For example, consider the sketch below. Using the information in the figure, the plate with 50 colonies would be chosen. 1.0 mL 1.0 mL 1.0 L 1.0 mL 1.0 mL A 0.1 ml de 2.0m00m 100 m 9.0 ml 9.0 ml 9.0 mi 9.9 ml 9.0 ml Original culture 1.0 ml 2.0...
4. You count 97 colonies on a plate inoculated with 2.5 ml of a 10* dilution of bacteria. How many CFU per ml of bacteria were in the uridiluted stock? (1 point)
4. From a culture of M. luteus, you take 10 L and dilute it into 9990uL of sterile water. From this dilution, you take an aliquot of 10uL and plate it onto fresh agar. The following day, you observe 96 colonies on the plate. What is the original CFU/mL of your culture of M. luteus?
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