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4. From a culture of M. luteus, you take 10 L and dilute it into 9990uL...
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
You have a flask from an overnight culture and take 1 mL from this flask and...
You have a flask from an overnight culture and take 1 mL from this flask and put it into 9 mL of PBS to dilute it (tube 1), you then take 1 mL from tube 1 and put that in a tube with 9 mL of PBS to make tube 2. You take 1 mL from tube 2 and put it in a tube with 9 mL of PBS to make tube 3. You take 1 mL from tube 3...
Hello, just wanted to check my answers. Answer as many as you like/can. Thankyou. 1) Four...
Hello, just wanted to check my answers. Answer as many as you like/can. Thankyou. 1) Four grams of soil are added to 36 ml sterile water and mixed well. 0.1 ml of this mix was added to 9.9 ml sterile water. This was diluted by 4 successive 1/10 dilutions. 1.0 ml from the last dilution was used to prepare a pour plate. After incubation 289 colonies were counted. Calculate the # CFU/g soil. 2) A 0.1 ml aliquot of a...
A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water....
A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution? b) How many cfu/mL were present in the original culture? c) How many...
help me with the math. m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or...
help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
What is the equation for calculating the cfu/ g soil? We used 1g soil and diluted...
What is the equation for calculating the cfu/ g soil? We used 1g soil and diluted it in 9mL of water --> is considered to be our 10-1 dilution. --> dilution factor is 10 We plated 0.1 mL of this 10-1 solution onto an agar plate. Can I use following equation to calculate the cfu/ g soil (density is neglected): cfu/g soil=(no.of colonies x dilution factor)/(volume of culture plate) cfu/g soil =( 30 cfu x 10) / 0.1 mL =...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions,...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways...
please help with whatever possible. thank you so much in advance. Name One use of serial...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates,...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates, resulting in the growth of an average of 68 colonies per plate. What is the concentration of colony-forming units (cfu/mi) present in the original culture? 0.1 ml 0.1 ml 1 ml 1 ml 100 ml 9.9 ml 9.9 ml 9 ml 9 ml 2 4 3. The culture flask at left contains a total of 3.2 x 10s cfu....
To dilute a bacterial culture, 100 mu l of a 16 hour culture is mixed with...
To dilute a bacterial culture, 100 mu l of a 16 hour culture is mixed with fresh culture media to a final volume of 5 ml. The 16 hour culture was diluted by a factor of: _____ An antibiotic (ampicillin) used during a Transformation experiment. Ampicillin is stored as a stock solution of 100 mg/ml. You are pouring plates from a 500 ml flask of Lauria Bertini agar (LB Agar) and you need an antibiotic concentration of 100 mu g/ml...
help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates, resulting in the growth of an average of 68 colonies per plate. What is the concentration of colony-forming units (cfu/mi) present in the original culture? 0.1 ml 0.1 ml 1 ml 1 ml 100 ml 9.9 ml 9.9 ml 9 ml 9 ml 2 4 3. The culture flask at left contains a total of 3.2 x 10s cfu....
To dilute a bacterial culture, 100 mu l of a 16 hour culture is mixed with fresh culture media to a final volume of 5 ml. The 16 hour culture was diluted by a factor of: _____ An antibiotic (ampicillin) used during a Transformation experiment. Ampicillin is stored as a stock solution of 100 mg/ml. You are pouring plates from a 500 ml flask of Lauria Bertini agar (LB Agar) and you need an antibiotic concentration of 100 mu g/ml...
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