You constructed your standard curve using calculated absorbance at 600 nm. Would it be accurate to...
You constructed your standard curve using calculated absorbance at 600 nm. Would it
be accurate to use your standard curve to determine the concentration of cells in LB
media based on an absorbance reading at 500 nm? Why or why not?
Homework Answers
No, it would not be appropriate to determine the concentration of cells at 500 nm using a curve drawn with Ab 600 values. This is because the absorptive properties of cells would differ between 500 nm and 600 nm and may not be directly correlated. One reason for this is that the cellular constituents that absorb at 600 nm may not absorb at 500 nm and the concentration of these substances might vary between cells at high density and low density.
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You constructed your standard curve using calculated absorbance
at 600 nm. Would it
be accurate to...
a) If a compound has the wavelength of maximum absorbance of 600 nm, what would happen...
a) If a compound has the wavelength of maximum absorbance of 600 nm, what would happen if all of the samples were measured at a slightly higher wavelength (i.e. 620 nm)? b) When plotting data of concentration vs. absorption of the compound, will the change in wavelength affect the R^2 value of the graph? why or why not? c) Will the change in wavelength affect the molar absorptivity calculated from the graph? why or why not?
Spectrophotometry help!? Would measuring the absorbance of the standard solutions used to prepare a calibration curve...
Spectrophotometry help!? Would measuring the absorbance of the standard solutions used to prepare a calibration curve and the unknown sample using a wavelength 10 nm higher or lower than lambda max provide an accurate concentration value for the unknown? Explain. If in an experiment, the pathlength of the light is 1 cm, and the concentration unit of the standard solutions is reported as mM, what would the units of the molar extinction coefficient, ε, be?
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs...
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...
please help with ALL questions thank you 1. Using excel, plot your standard curve based on...
please help with ALL questions thank you
1. Using excel, plot your standard curve based on Table 9-1 and calculate the line of best fit. Put concentration in the x column and OD in the y to solve for the concentrations of your unknown in Table 9-3. Report the slope and the y-intercept below. (1 mark) Slope (m) = v-intercept(b) = 2. Can this standard curve for yeast cells be used for cali cells (why/ why not?) (2 marks) 3....
Absorption [%] 300 400 500 600 700 nm What wavelength of light would be the best...
Absorption [%] 300 400 500 600 700 nm What wavelength of light would be the best to use when constructing a standard curve for this pigment? 400 nm 450 nm 500 nm 550 nm 600 nm
The following curve presents the relationship between BSA concentration and OD at 595 or 600 nm....
The following curve presents the relationship between BSA concentration and OD at 595 or 600 nm. You isolated a recombinant protein of unknown concentration. You measured the OD value of the isolated protein and found that its OD is 0.5. Using the following BSA standard curve, determine the concentration of the isolated protein in mg/ml. BSA Standard Curve 0,9 0,8 0,7 0,6 0.5 0,4 0,3 0,2 0,1 y 0,4299x 0,0218 R" # 0,99697 0,1 0,5 1,5 2,5 mg/ml
Which method do you think would be the most accurate in determining original concentration of cells...
Which method do you think would be the most accurate in determining original concentration of cells in a culture? Why do you think that one is the most accurate method? plate count method? standard curve?
Post LaboratorY Qustions A colored salution had a large absorbance peak at 690 nm Use the...
Post LaboratorY Qustions A colored salution had a large absorbance peak at 690 nm Use the visitle spectrum in your text or other reliable source to determine the color of this solution 2 A soft drink had a tertrazine also known as yellow #5 concentration of 1.75 x 10 M If a person drank two 12 ounce cans of this beverage for a vear (365 days). What mass of tartrazine would the person consume? Moleculer weight 534 g/mol for tartrazine....
3. In Part A, you measure the absorbance of four FesCN" solutions and prepare a plot...
3. In Part A, you measure the absorbance of four FesCN" solutions and prepare a plot (Absorbance vs. concentration) similar to the one below. Beer's Law Plot for FeSCN2 0.6 0.5 0.4 0.3 d 0.2 0.1 0 y = 2417.5× + 0.0483 R2 0.99897 0.00025 0.00015 0.0002 0.00005 0.0001 0 Concentration (M) You use this plot as a standard curve in Part B to determine the concentration of an unknown. If an unknown FeSCN2 solution gives an absorbance reading of...
please answer 1 and 2 table 4 protein concentrations 1. Determine the protein concentrations for your...
please answer 1 and 2
table
4
protein
concentrations
1. Determine the protein concentrations for your positive and negative control and your test sample (Table 4) using the protein standard curve you constructed. Include Table 4 and your protein concentrations in your response. Do these results seem accurate to you? Did they differ from what you expected for the food item you brought into class? 2. What is the R2 value of your known protein concentrations plotted against their absorbency?...
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...
please help with ALL questions thank you
1. Using excel, plot your standard curve based on Table 9-1 and calculate the line of best fit. Put concentration in the x column and OD in the y to solve for the concentrations of your unknown in Table 9-3. Report the slope and the y-intercept below. (1 mark) Slope (m) = v-intercept(b) = 2. Can this standard curve for yeast cells be used for cali cells (why/ why not?) (2 marks) 3....
Absorption [%] 300 400 500 600 700 nm What wavelength of light would be the best to use when constructing a standard curve for this pigment? 400 nm 450 nm 500 nm 550 nm 600 nm
The following curve presents the relationship between BSA concentration and OD at 595 or 600 nm. You isolated a recombinant protein of unknown concentration. You measured the OD value of the isolated protein and found that its OD is 0.5. Using the following BSA standard curve, determine the concentration of the isolated protein in mg/ml. BSA Standard Curve 0,9 0,8 0,7 0,6 0.5 0,4 0,3 0,2 0,1 y 0,4299x 0,0218 R" # 0,99697 0,1 0,5 1,5 2,5 mg/ml
Post LaboratorY Qustions A colored salution had a large absorbance peak at 690 nm Use the visitle spectrum in your text or other reliable source to determine the color of this solution 2 A soft drink had a tertrazine also known as yellow #5 concentration of 1.75 x 10 M If a person drank two 12 ounce cans of this beverage for a vear (365 days). What mass of tartrazine would the person consume? Moleculer weight 534 g/mol for tartrazine....
3. In Part A, you measure the absorbance of four FesCN" solutions and prepare a plot (Absorbance vs. concentration) similar to the one below. Beer's Law Plot for FeSCN2 0.6 0.5 0.4 0.3 d 0.2 0.1 0 y = 2417.5× + 0.0483 R2 0.99897 0.00025 0.00015 0.0002 0.00005 0.0001 0 Concentration (M) You use this plot as a standard curve in Part B to determine the concentration of an unknown. If an unknown FeSCN2 solution gives an absorbance reading of...
please answer 1 and 2
table
4
protein
concentrations
1. Determine the protein concentrations for your positive and negative control and your test sample (Table 4) using the protein standard curve you constructed. Include Table 4 and your protein concentrations in your response. Do these results seem accurate to you? Did they differ from what you expected for the food item you brought into class? 2. What is the R2 value of your known protein concentrations plotted against their absorbency?...
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