Question

The following curve presents the relationship between BSA concentration and OD at 595 or 600 nm. You isolated a recombinant protein of unknown concentration. You measured the OD value of the isolated protein and found that its OD is 0.5. Using the following BSA standard curve, determine the concentration of the isolated protein in mg/ml.

Answer 1

Sol)

According to question   we know the formula for calculating the concentration of protein by taking the reference BSA we generally use the following formula

y= 0.052(X) - 0.120

Now, we have to find the “x” value

X for concentration of unknown sample (protein) in mg/ml

y= 0.052 X - 0.120

0.052 X=y+0.120

X=y+0.120/0.052

Here, y=0.5

X=0.5+0.120/0.052

X=0.5+2.307

X=2.807 mg/ml(concentration of protein)