Question

Which method do you think would be the most accurate in determining original concentration of cells in a culture? Why do you think that one is the most accurate method?

plate count method?

standard curve?

Answer 1

Standard curve method is better than plate count method. This is because the former gives the total number of cell count in a sample whereas the latter tells us only about the viable cells.

Standard curve is cell counting method based on the absorbance of sample.

Plate count method is based upon the number of CFU.

Petroff Hausser chamber CFU method Direct counting method Indirect counting method Counts both viable and inviable cells Counts only viable cells Direct dilution is done Serial dilution is done No incubation time is required Incubation on a nutrient media is required Number of cells per uL can be counted easily Number of Colony forming units per mL is counted

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