Why is the plate count method a determination of the number of viable cells? Which method...
Why is the plate count method a determination of the number of viable cells?
Which method do you think would be the most accurate in determining original concentration of cells in a culture? Why do you think that one is the most accurate method?
Homework Answers
In order to count the number of growing cells in a population a
viable cell count method is performed. The count method depends on
the colony formed by the bacteria when grown in nutrient medium.
the colonies are visible and one can easily count the number of
colonies by their naked eye
Plate count method is the accurate method for the determination of
microbial count.
On the plate containing culture medium, food sample is placed and
after the period of incubation the the person can easily count the
number of formation of colonies on plate containing culture
medium.
stay safe. For doubt ask in comments
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Why is the plate count method a determination of the
number of viable cells?
Which method...
Which method do you think would be the most accurate in determining original concentration of cells...
Which method do you think would be the most accurate in determining original concentration of cells in a culture? Why do you think that one is the most accurate method? plate count method? standard curve?
please help with ALL questions thank you 1. Using excel, plot your standard curve based on...
please help with ALL questions thank you
1. Using excel, plot your standard curve based on Table 9-1 and calculate the line of best fit. Put concentration in the x column and OD in the y to solve for the concentrations of your unknown in Table 9-3. Report the slope and the y-intercept below. (1 mark) Slope (m) = v-intercept(b) = 2. Can this standard curve for yeast cells be used for cali cells (why/ why not?) (2 marks) 3....
can anyone please help me answer these 3 biology questions? 20. The plate count method allows...
can anyone please help me answer these 3 biology
questions?
20. The plate count method allows direct count of total cells growing on solid medium. A. True B. False 21. Which of the following is a statistical procedure for estimating the number of viable microorganisms in a sample? A. Electronic cell counting device B. Most probable number (MPN) C. Petroff-Hausser chamber D. Spectrophotometry E. Viable plate count 22. Which of the following is the most commonly used to estimate and...
you count 187 CFUS on a spread plate. The plate was prepared by spreading 0.2ml of...
you count 187 CFUS on a spread plate. The plate was prepared by spreading 0.2ml of a 1:100 dilution of a 1:50 dilution of a 1:10 dilution of the original sample. What is the concentration of the original sample. What is the concentration of the original culture in cells/ml
please type up the answers. Conclusions. Questions for further thought and comprehension: 1. What did you...
please type up the answers.
Conclusions. Questions for further thought and comprehension: 1. What did you determine to be the concentration of the original bacterial culture? Do you think your results are accurate? Explain why or why not. 2. Did you have a countable number of colonies on more than one of your plates? Assume you had two plates that had countable numbers of colonies, one with 124 colonies (and a dilution factor of 1/1.000) and one with 11 colonies...
You have been given a yeast suspension to count. You count the number of yeast cells...
You have been given a yeast suspension to count. You count the number of yeast cells in 7 of the 0.04mm2 boxes of the central grid of the hemocytometer. Your counting results are: 20, 18, 19, 21, 22, 17, and 22. Assuming there has been no dilution and you are counting the original sample, determine the concentration of cells in the original sample Prepare a set of serial dilutions of the yeast suspension for a viable count. Write and draw...
17.A viable count of this culture, which represents Staphylococcus aureus, was done after 120 minutes. How...
17.A viable count of this culture, which represents Staphylococcus aureus, was done after 120 minutes. How many CFUs are expected if you plated 0.2 mL of a 104 dilution? (Hint: Consider the average number of cells per CFU) 18. At 65 minutes, the cells were transferred to a new medium in which the growth rate (H) was three times lower. What is the expected number of cells after 60 minutes of growth following the transfer? 19.By what factor did the...
> Which enumeration technique would be best for a very old culture? A) Viable plate counting...
> Which enumeration technique would be best for a very old culture? A) Viable plate counting B) Direct counting with a haemocytometer C) Optical density measurements using a spectrophotometer D) Streak plating
9. Suppose you ingest 20 cells of Salmonella typhimurium in a chicken taco. Some of the...
9. Suppose you ingest 20 cells of Salmonella typhimurium in a chicken taco. Some of the bacteria are killed as they pass through the stomach but 10 viable cells survive and reach the intestine. Suppose further that Salmonella generation time in the intestine is 2.7 hours and that you will feel sick when there are 1,000,000 bacteria. How much time will elapse before you feel sick? Show your work. 10. You are doing a viable cell count on a bacterial...
Direct counts of cells in liquid samples can be performed using a Petroff-Hausser counting chamber. Research...
Direct counts of cells in liquid samples can be performed using a Petroff-Hausser counting chamber. Research this method and describe how it compares to the viable plate count method of determining the number of CFU in a sample.
please help with ALL questions thank you
1. Using excel, plot your standard curve based on Table 9-1 and calculate the line of best fit. Put concentration in the x column and OD in the y to solve for the concentrations of your unknown in Table 9-3. Report the slope and the y-intercept below. (1 mark) Slope (m) = v-intercept(b) = 2. Can this standard curve for yeast cells be used for cali cells (why/ why not?) (2 marks) 3....
can anyone please help me answer these 3 biology
questions?
20. The plate count method allows direct count of total cells growing on solid medium. A. True B. False 21. Which of the following is a statistical procedure for estimating the number of viable microorganisms in a sample? A. Electronic cell counting device B. Most probable number (MPN) C. Petroff-Hausser chamber D. Spectrophotometry E. Viable plate count 22. Which of the following is the most commonly used to estimate and...
please type up the answers.
Conclusions. Questions for further thought and comprehension: 1. What did you determine to be the concentration of the original bacterial culture? Do you think your results are accurate? Explain why or why not. 2. Did you have a countable number of colonies on more than one of your plates? Assume you had two plates that had countable numbers of colonies, one with 124 colonies (and a dilution factor of 1/1.000) and one with 11 colonies...
17.A viable count of this culture, which represents Staphylococcus aureus, was done after 120 minutes. How many CFUs are expected if you plated 0.2 mL of a 104 dilution? (Hint: Consider the average number of cells per CFU) 18. At 65 minutes, the cells were transferred to a new medium in which the growth rate (H) was three times lower. What is the expected number of cells after 60 minutes of growth following the transfer? 19.By what factor did the...
> Which enumeration technique would be best for a very old culture? A) Viable plate counting B) Direct counting with a haemocytometer C) Optical density measurements using a spectrophotometer D) Streak plating
9. Suppose you ingest 20 cells of Salmonella typhimurium in a chicken taco. Some of the bacteria are killed as they pass through the stomach but 10 viable cells survive and reach the intestine. Suppose further that Salmonella generation time in the intestine is 2.7 hours and that you will feel sick when there are 1,000,000 bacteria. How much time will elapse before you feel sick? Show your work. 10. You are doing a viable cell count on a bacterial...
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