You have been given a yeast suspension to count. You count the number of yeast cells...
- You have been given a yeast suspension to count. You count the number of yeast cells in 7 of the 0.04mm2 boxes of the central grid of the hemocytometer. Your counting results are: 20, 18, 19, 21, 22, 17, and 22. Assuming there has been no dilution and you are counting the original sample, determine the concentration of cells in the original sample
- Prepare a set of serial dilutions of the yeast suspension for a viable count. Write and draw your dilution calculation and plan here. Be sure to show what volume you are transferring in each dilution and the volume present in each of the sterile dilution blanks.
Homework Answers
For serial dilution make a series of two fold dilution until we have a tube that is clear. Transfer 0.1ml of the suspension from each tube to the next begining with the one that is containing the cells.
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You have been given a yeast suspension to count. You count the
number of yeast cells...
In order to count cells, the culture must be duted The technique o serialul precise and...
In order to count cells, the culture must be duted The technique o serialul precise and quantitative method alle e n al b e performed allow you to determine how many well per miller were the time the were made in overview knowvolume of culturedded to know Volume 0 sterile diluent aliquid that will not harm the cell The liquid in this tube is mixed and known volume of this culture is transferred to another known volume of sterile diluent...
Question 6 Lab 16 Report 5. You are provided with a yeast culture that is very...
Question 6
Lab 16 Report 5. You are provided with a yeast culture that is very turbid. You are asked to make 10-fold dilutions of the culture. You are given tap water, test tubes that hold maximum volume of 10 ml, and a 1 ml pipette for your work. How would you make this dilution? Fill in the blanks (Red and Blue) on the figure to answer this question. 1ml 1ml 1ml 1ml 1:10 1:100 1:1000 1:10000 my mi water...
Please show how to do these enumeration questions by showing the work. What is the dilution...
Please show how to do these enumeration questions by showing the work. What is the dilution factor if you add 625 µL of culture to 4.375 mL of dilution medium? You count the 10-3 dilution of a yeast suspension using the 1/25 mm2 boxes : you find 45 cells in box 1, 15 cells in box 2, 42 cells in box 3, 23 cells in box 4, and 26 cells in box 5. a) What is the original concentration of...
9. Suppose you ingest 20 cells of Salmonella typhimurium in a chicken taco. Some of the...
9. Suppose you ingest 20 cells of Salmonella typhimurium in a chicken taco. Some of the bacteria are killed as they pass through the stomach but 10 viable cells survive and reach the intestine. Suppose further that Salmonella generation time in the intestine is 2.7 hours and that you will feel sick when there are 1,000,000 bacteria. How much time will elapse before you feel sick? Show your work. 10. You are doing a viable cell count on a bacterial...
1. You prepared a dilution 1 in 2 of trypsinized CHO-K1 cells. Then, you took an...
1. You prepared a dilution 1 in 2 of trypsinized CHO-K1 cells. Then, you took an aliquot of the cell suspension and counted the cells using a hemocytometer. There were 75 cells in the 16 little squares. Please explain how to get each! a. Calculate the concentration of the cells prior to the dilution. Show your calculations. Use scientific notation to express the result. b. Now, you want to seed a 25cm2 flask with 750,000 cells. Calculate how many ml...
You have performed a serial dilution of a sample with an unknown concentration of microorganisms. You...
You have performed a serial dilution of a sample with an unknown concentration of microorganisms. You counted 55 CFU on a countable plate inoculated with 0.1 mL of a 10-3 dilution. What was the population density of the original sample per milliliter? Many of the quantitative methods discussed are popular because they enable the microbiology researcher to selectively count live cells only. Why do you think this might be an important or desirable feature in a medical or environmental research...
Your colleague in the laboratory was able to collect all his raw data from a growth curve on the Hela S3 cell line
Your colleague in the laboratory was able to collect all his raw data from a growth curve on the Hela S3 cell line, but had to leave unexpectedly and has asked you to perform cell calculations to show your professor. This what your colleague did: he seeded cells into a 24-well plate (allows for replicate sampling and it is much easier to handle one 24-well plate rather than 24 flasks), and on each of the days indicated in the table...
please help with ALL questions thank you 1. Using excel, plot your standard curve based on...
please help with ALL questions thank you
1. Using excel, plot your standard curve based on Table 9-1 and calculate the line of best fit. Put concentration in the x column and OD in the y to solve for the concentrations of your unknown in Table 9-3. Report the slope and the y-intercept below. (1 mark) Slope (m) = v-intercept(b) = 2. Can this standard curve for yeast cells be used for cali cells (why/ why not?) (2 marks) 3....
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
Answer and do the following calculations: 1. Suppose your professor handed you a test tube with...
Answer and do the following calculations: 1. Suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10–2 dilution of the entire culture. Explain how you would do this. Show your calculations. 2. How would you produce a 10–1 dilution of a 3 mL bacterial sample using the entire 3 mL volume? 3. You have 0.05 mL of an undiluted culture at a concentration of...
In order to count cells, the culture must be duted The technique o serialul precise and quantitative method alle e n al b e performed allow you to determine how many well per miller were the time the were made in overview knowvolume of culturedded to know Volume 0 sterile diluent aliquid that will not harm the cell The liquid in this tube is mixed and known volume of this culture is transferred to another known volume of sterile diluent...
Question 6
Lab 16 Report 5. You are provided with a yeast culture that is very turbid. You are asked to make 10-fold dilutions of the culture. You are given tap water, test tubes that hold maximum volume of 10 ml, and a 1 ml pipette for your work. How would you make this dilution? Fill in the blanks (Red and Blue) on the figure to answer this question. 1ml 1ml 1ml 1ml 1:10 1:100 1:1000 1:10000 my mi water...
9. Suppose you ingest 20 cells of Salmonella typhimurium in a chicken taco. Some of the bacteria are killed as they pass through the stomach but 10 viable cells survive and reach the intestine. Suppose further that Salmonella generation time in the intestine is 2.7 hours and that you will feel sick when there are 1,000,000 bacteria. How much time will elapse before you feel sick? Show your work. 10. You are doing a viable cell count on a bacterial...
please help with ALL questions thank you
1. Using excel, plot your standard curve based on Table 9-1 and calculate the line of best fit. Put concentration in the x column and OD in the y to solve for the concentrations of your unknown in Table 9-3. Report the slope and the y-intercept below. (1 mark) Slope (m) = v-intercept(b) = 2. Can this standard curve for yeast cells be used for cali cells (why/ why not?) (2 marks) 3....
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