Homework Answers
Request Answer!
We need at least 10 more requests to produce the answer.
0 / 10 have requested this problem solution
The more requests, the faster the answer.
Add Answer to:
Question 6
Lab 16 Report 5. You are provided with a yeast culture that is very...
o Section 5 You are provided with a yeast culture that is very turbid. You are...
o Section 5 You are provided with a yeast culture that is very turbid. You are asked to make 10-fold dilutions of the culture. You are given distilled water, test tubes that hold a maximum volume of 10 ml, and a 1 ml pipette for your work. How would you make this dilution? Fill in the blanks on the figure to answer this question. mi 5 ml ml ml Iml a ml mi HO Yeast suspension Dilution: Dilution: Dilution: Dilution:
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
20) Six tubes are in a rack.. You mix 1ml of cell culture with 0.5 ml...
20) Six tubes are in a rack.. You mix 1ml of cell culture with 0.5 ml of water in tube #1, transfer 0.1 ml of tube #1 to tube #2 containing 0.9ml of water and mix well and the same procedure (0.1 ml of culture +0.9 ml of water) is repeated for the remaining 4 tubes. After plating 0.1 ml of the last dilution tube #6, you obtain 100 colonies. what is the cell density (cfu/ml) of the original cell...
You have a bacterial culture solution. When you directly measured the OD600, of the culture solution,...
You have a bacterial culture solution. When you directly
measured the OD600, of the culture solution, it was 3. Then, you
performed a 10-fold dilution and measured the OD600
of the diluted solution, which was 0.5. Thirdly, you performed
another 10-fold dilution and measured the OD600 of the diluted
solution, which was 0.07. Assuming that OD
600 =1 is equivalent to 10^9 cells/ml, how many cells do you
have in 1ml of the original culture?
You have a bacterial culture...
Please show how to do these enumeration questions by showing the work. What is the dilution...
Please show how to do these enumeration questions by showing the work. What is the dilution factor if you add 625 µL of culture to 4.375 mL of dilution medium? You count the 10-3 dilution of a yeast suspension using the 1/25 mm2 boxes : you find 45 cells in box 1, 15 cells in box 2, 42 cells in box 3, 23 cells in box 4, and 26 cells in box 5. a) What is the original concentration of...
Need the answers to all please :) 1) You recently purchased a new mitogen at 1mg/1ml...
Need the answers to all please :) 1) You recently purchased a new mitogen at 1mg/1ml (stock) and then prepared 1:100 dilutions as a working solution (tube A). The company also suggest verifying the optimal dilution for your experiment by doing serial (twofold) dilutions. Describe how you would prepare 500microliters of working solution (tube A). What volume of inhibitor will you add in microliter? 2) You would like to optimize the working concentration of the inhibitor, and therefore perform two-fold...
phenol is in each of your unknowns. 1. You will be given a nitrophenol solution of...
phenol is in each of your unknowns. 1. You will be given a nitrophenol solution of concentration 400 nmoles/mL and you will use this stock solution to make a serial dilution as follows: To make the serial dilution you need to prepare 5 tubes with 1 ml water. Next, add 1ml from the 400 nmoles/ml stock to the first tube. Pipette the solution up and down carefully and remove 1 ml of this solution to the next tube. Repeat this...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions,...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways...
Hi I am im a biochemistry lab and we are doing an experiment titled: PCR Amplification...
Hi
I am im a biochemistry lab and we are doing an experiment titled:
PCR Amplification of LeuRS F C Terminal Domain. I am having
troubles figuring out the rest of my caluclations.
I already did the calculations for the first dilution set, I
was wondering how I would do the calculation for the second
dilution set.
Also I was wondering how I would determine the theoretical
values for my data. Is there a specific formula I can use?
If...
Please answer both! I would appreciate it! Show all your work as well. I’m having trouble...
Please answer both! I would appreciate it! Show all your work
as well. I’m having trouble with dilutions. Thank you!
You have 99 mL and 9 mL dilution blanks available to you. Draw and label a protocol by which you could make three plates that contained a 1 X 10, 1 X 104 and 1 X、105 dilution. 2. 3. A culture was diluted by adding a 0.1 mL aliquot to 0.9 mL water. Then, 0.1 mL of this dilution was...
o Section 5 You are provided with a yeast culture that is very turbid. You are asked to make 10-fold dilutions of the culture. You are given distilled water, test tubes that hold a maximum volume of 10 ml, and a 1 ml pipette for your work. How would you make this dilution? Fill in the blanks on the figure to answer this question. mi 5 ml ml ml Iml a ml mi HO Yeast suspension Dilution: Dilution: Dilution: Dilution:
You have a bacterial culture solution. When you directly
measured the OD600, of the culture solution, it was 3. Then, you
performed a 10-fold dilution and measured the OD600
of the diluted solution, which was 0.5. Thirdly, you performed
another 10-fold dilution and measured the OD600 of the diluted
solution, which was 0.07. Assuming that OD
600 =1 is equivalent to 10^9 cells/ml, how many cells do you
have in 1ml of the original culture?
You have a bacterial culture...
phenol is in each of your unknowns. 1. You will be given a nitrophenol solution of concentration 400 nmoles/mL and you will use this stock solution to make a serial dilution as follows: To make the serial dilution you need to prepare 5 tubes with 1 ml water. Next, add 1ml from the 400 nmoles/ml stock to the first tube. Pipette the solution up and down carefully and remove 1 ml of this solution to the next tube. Repeat this...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways...
Hi
I am im a biochemistry lab and we are doing an experiment titled:
PCR Amplification of LeuRS F C Terminal Domain. I am having
troubles figuring out the rest of my caluclations.
I already did the calculations for the first dilution set, I
was wondering how I would do the calculation for the second
dilution set.
Also I was wondering how I would determine the theoretical
values for my data. Is there a specific formula I can use?
If...
Please answer both! I would appreciate it! Show all your work
as well. I’m having trouble with dilutions. Thank you!
You have 99 mL and 9 mL dilution blanks available to you. Draw and label a protocol by which you could make three plates that contained a 1 X 10, 1 X 104 and 1 X、105 dilution. 2. 3. A culture was diluted by adding a 0.1 mL aliquot to 0.9 mL water. Then, 0.1 mL of this dilution was...
Most questions answered within 3 hours.
-
Under common law, right of survivorship was automatically a
feature of which type of co-tenancy?
a....
asked 5 minutes ago -
Are there such things as microscopic multicellular animal
parasites? If so, please give examples.
asked 4 minutes ago -
Problem 03.019 Annual Worth Calculations
Find the value of x that makes the equivalent annual
worth...
asked 9 minutes ago -
At 1 bar, how much energy is required to heat 61.0 g of H2O(s)
at −12.0...
asked 26 minutes ago -
Find the mixed-strategy equilibrium to the Battle of the sexes
game in Figure 5.1 below
Hockey...
asked 28 minutes ago -
Use the following information to answer the next three
questions.
QUESTION 5
As of today, the...
asked 33 minutes ago -
Using the specific identification method: Date Units purchased
Cost per unit Ending inventory March 1 15...
asked 36 minutes ago -
PLEASE HELP, NO ONE IS ANSWERING MY QUESTION AND IT IS SUE TODAY
WORTH 20% OF...
asked 50 minutes ago -
α = 0.0007889 T, I = 2.9 A
Other Magnetic Fields: First, based on your
value...
asked 50 minutes ago -
This assignment is a continuation of the 2nd one. You as a HR
Manager, select an...
asked 52 minutes ago -
Hastings Entertainment has a beta of 0.64. If the market return
is expected to be 13.80...
asked 1 hour ago -
9. Depository institutions are always:
a. illiquid
b. profitable
c. insolvent
d. all of the above...
asked 1 hour ago