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o Section 5 You are provided with a yeast culture that is very turbid. You are...
Question 6 Lab 16 Report 5. You are provided with a yeast culture that is very...
Question 6
Lab 16 Report 5. You are provided with a yeast culture that is very turbid. You are asked to make 10-fold dilutions of the culture. You are given tap water, test tubes that hold maximum volume of 10 ml, and a 1 ml pipette for your work. How would you make this dilution? Fill in the blanks (Red and Blue) on the figure to answer this question. 1ml 1ml 1ml 1ml 1:10 1:100 1:1000 1:10000 my mi water...
You are given a culture that has a concentration of 2.1x10^9 cfu.ml. you are asked to...
You are given a culture that has a concentration of 2.1x10^9 cfu.ml. you are asked to dilute it and plate it so that you have 270 colonies on one plate. you are given plenty diluent but only 4 tubes with which to make your dilutions. Demonstrate how you would set up the dilution. For each step indicate in an orderly fashion the V2, V1, Diluent volume, dilution, c-dilution, DF, CDF up to that step and the concentration of each tube.
Please show how to do these enumeration questions by showing the work. What is the dilution...
Please show how to do these enumeration questions by showing the work. What is the dilution factor if you add 625 µL of culture to 4.375 mL of dilution medium? You count the 10-3 dilution of a yeast suspension using the 1/25 mm2 boxes : you find 45 cells in box 1, 15 cells in box 2, 42 cells in box 3, 23 cells in box 4, and 26 cells in box 5. a) What is the original concentration of...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
Hi I am im a biochemistry lab and we are doing an experiment titled: PCR Amplification...
Hi
I am im a biochemistry lab and we are doing an experiment titled:
PCR Amplification of LeuRS F C Terminal Domain. I am having
troubles figuring out the rest of my caluclations.
I already did the calculations for the first dilution set, I
was wondering how I would do the calculation for the second
dilution set.
Also I was wondering how I would determine the theoretical
values for my data. Is there a specific formula I can use?
If...
Suppose you would like to determine the number of bacteria in a culture via an aerobic...
Suppose you would like to determine the number of bacteria in a culture via an aerobic plate count. Explain (with pictures) how you would set up a dilution series (104, 106, 10) using the fewest number of dilution blanks. Draw and label your tubes and plates with each dilution. Show all volumes including transfers, amounts in tubes, and amounts plated 1. You want to set up a 1:10 dilution series, so that you can plate a 10-1, 102, and a...
VPC 2. You have received a sample from a sewage treatment plant, and have been asked...
VPC 2. You have received a sample from a sewage treatment plant, and have been asked to determine how many CFU/ml are in this sample. You want to make a 1/100,000 fold dilution, but the smallest volume you can measure is 1.0 ml, and the tubes available to you only hold 10 ml. Draw a scheme showing how you would do this.
phenol is in each of your unknowns. 1. You will be given a nitrophenol solution of...
phenol is in each of your unknowns. 1. You will be given a nitrophenol solution of concentration 400 nmoles/mL and you will use this stock solution to make a serial dilution as follows: To make the serial dilution you need to prepare 5 tubes with 1 ml water. Next, add 1ml from the 400 nmoles/ml stock to the first tube. Pipette the solution up and down carefully and remove 1 ml of this solution to the next tube. Repeat this...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions,...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways...
Serial dilution problem You have a stock of 5% SDS. You take some of that stock...
Serial dilution problem You have a stock of 5% SDS. You take some of that stock and dilute it 4-fold in tube #1. the tubes hold 1.5ml. give an example of how much 5% stock and how much water you would use to make 4 fold dilution - volume of 5% stock ? - volume of water? - How much volume transferred? - if you took a sample out of tube 2 and did another 4 fold dilution, what concentration...
Question 6
Lab 16 Report 5. You are provided with a yeast culture that is very turbid. You are asked to make 10-fold dilutions of the culture. You are given tap water, test tubes that hold maximum volume of 10 ml, and a 1 ml pipette for your work. How would you make this dilution? Fill in the blanks (Red and Blue) on the figure to answer this question. 1ml 1ml 1ml 1ml 1:10 1:100 1:1000 1:10000 my mi water...
Hi
I am im a biochemistry lab and we are doing an experiment titled:
PCR Amplification of LeuRS F C Terminal Domain. I am having
troubles figuring out the rest of my caluclations.
I already did the calculations for the first dilution set, I
was wondering how I would do the calculation for the second
dilution set.
Also I was wondering how I would determine the theoretical
values for my data. Is there a specific formula I can use?
If...
Suppose you would like to determine the number of bacteria in a culture via an aerobic plate count. Explain (with pictures) how you would set up a dilution series (104, 106, 10) using the fewest number of dilution blanks. Draw and label your tubes and plates with each dilution. Show all volumes including transfers, amounts in tubes, and amounts plated 1. You want to set up a 1:10 dilution series, so that you can plate a 10-1, 102, and a...
VPC 2. You have received a sample from a sewage treatment plant, and have been asked to determine how many CFU/ml are in this sample. You want to make a 1/100,000 fold dilution, but the smallest volume you can measure is 1.0 ml, and the tubes available to you only hold 10 ml. Draw a scheme showing how you would do this.
phenol is in each of your unknowns. 1. You will be given a nitrophenol solution of concentration 400 nmoles/mL and you will use this stock solution to make a serial dilution as follows: To make the serial dilution you need to prepare 5 tubes with 1 ml water. Next, add 1ml from the 400 nmoles/ml stock to the first tube. Pipette the solution up and down carefully and remove 1 ml of this solution to the next tube. Repeat this...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways...
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