Homework Answers
I will take 1 ml from sewage sample and will mix it to 9 mil of
distilled water. the dilution would be 1/10. Then i will take 1 ml
from this mixture and again will mix it with 9 ml fresh water. so
resulting dilution will be 1/100. After that I will take 1 ml from
this mixture and add 9 ml water to it. so the dilution we get will
be 1/1000. Similarly i will take again 1 ml rom this solution and
will add 9 ml of fresh water into it. resulting dilution will be
1/10000. Finally i will take 1 ml from this and add 9 ml fresh
water into it. Then we gt 1/100000 dilution. The serial dilution is
explained in the diagram below:
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VPC 2. You have received a sample from a sewage treatment plant, and have been asked...
(A) You have a sample with an original concentration of 1.0 x 10^7 CFU/mL. With the...
(A) You have a sample with an original concentration of 1.0 x 10^7 CFU/mL. With the Plate Count Method, what final dilution factor would be needed to produce countable plates? Show your work. (B) Describe a dilution scheme (how many tubes, what volume in each tube, what DF is achieved in each step) that uses only the 9-mL blank diluent tubes to achieve the dilution needed for this FDF.
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
Serial dilution problem You have a stock of 5% SDS. You take some of that stock...
Serial dilution problem You have a stock of 5% SDS. You take some of that stock and dilute it 4-fold in tube #1. the tubes hold 1.5ml. give an example of how much 5% stock and how much water you would use to make 4 fold dilution - volume of 5% stock ? - volume of water? - How much volume transferred? - if you took a sample out of tube 2 and did another 4 fold dilution, what concentration...
You have collected water samples of the influent (raw sewage) and effluent (treated sewage) at Houston Wastewater Treatment Plant. You plan to conduct the BOD5 test to determine the BOD rate constant,...
You have collected water samples of the influent (raw sewage) and effluent (treated sewage) at Houston Wastewater Treatment Plant. You plan to conduct the BOD5 test to determine the BOD rate constant, k. A. Would you expect the rate constants to be the same or different in these two samples? If different, which would be higher and why? B. If the BOD5 for the raw sewage sample is 600 mg/L, and the ultimate BOD is 650 mg/L at 20oC, find...
o Section 5 You are provided with a yeast culture that is very turbid. You are...
o Section 5 You are provided with a yeast culture that is very turbid. You are asked to make 10-fold dilutions of the culture. You are given distilled water, test tubes that hold a maximum volume of 10 ml, and a 1 ml pipette for your work. How would you make this dilution? Fill in the blanks on the figure to answer this question. mi 5 ml ml ml Iml a ml mi HO Yeast suspension Dilution: Dilution: Dilution: Dilution:
help me with the math. m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or...
help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The...
1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The final volume should be 100mL. Which of the following outlines the correct way to make this dilution? For the other answer options, explain why they are incorrect. A. 10mL of patient sample added to 40mL of saline B. 10mL of patient sample added to 50mL of saline C. 20mL of patient sample added to 100mL of saline D. 20mL of patient sample added to...
Question 6 Lab 16 Report 5. You are provided with a yeast culture that is very...
Question 6
Lab 16 Report 5. You are provided with a yeast culture that is very turbid. You are asked to make 10-fold dilutions of the culture. You are given tap water, test tubes that hold maximum volume of 10 ml, and a 1 ml pipette for your work. How would you make this dilution? Fill in the blanks (Red and Blue) on the figure to answer this question. 1ml 1ml 1ml 1ml 1:10 1:100 1:1000 1:10000 my mi water...
Answer and do the following calculations: 1. Suppose your professor handed you a test tube with...
Answer and do the following calculations: 1. Suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10–2 dilution of the entire culture. Explain how you would do this. Show your calculations. 2. How would you produce a 10–1 dilution of a 3 mL bacterial sample using the entire 3 mL volume? 3. You have 0.05 mL of an undiluted culture at a concentration of...
While interning at a wastewater treatment plant, you conduct a seeded BOD5 test of the lant's...
While interning at a wastewater treatment plant, you conduct a seeded BOD5 test of the lant's effluent. For the experimental bottle, you insert 30 mL of test sample into the 300-mL ottle and fill the rest with seeded dilution water. You fill a blank bottle with seeded dilution ater. You measure the following dissolved oxygen (DO) concentrations at the start and end f the 5-day test: Bottle Initial DO (mg/L) Final DO (mg/L) Experimental 8.1 4.6 Blank 7.8 6.7 hat...
o Section 5 You are provided with a yeast culture that is very turbid. You are asked to make 10-fold dilutions of the culture. You are given distilled water, test tubes that hold a maximum volume of 10 ml, and a 1 ml pipette for your work. How would you make this dilution? Fill in the blanks on the figure to answer this question. mi 5 ml ml ml Iml a ml mi HO Yeast suspension Dilution: Dilution: Dilution: Dilution:
help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The final volume should be 100mL. Which of the following outlines the correct way to make this dilution? For the other answer options, explain why they are incorrect. A. 10mL of patient sample added to 40mL of saline B. 10mL of patient sample added to 50mL of saline C. 20mL of patient sample added to 100mL of saline D. 20mL of patient sample added to...
Question 6
Lab 16 Report 5. You are provided with a yeast culture that is very turbid. You are asked to make 10-fold dilutions of the culture. You are given tap water, test tubes that hold maximum volume of 10 ml, and a 1 ml pipette for your work. How would you make this dilution? Fill in the blanks (Red and Blue) on the figure to answer this question. 1ml 1ml 1ml 1ml 1:10 1:100 1:1000 1:10000 my mi water...
While interning at a wastewater treatment plant, you conduct a seeded BOD5 test of the lant's effluent. For the experimental bottle, you insert 30 mL of test sample into the 300-mL ottle and fill the rest with seeded dilution water. You fill a blank bottle with seeded dilution ater. You measure the following dissolved oxygen (DO) concentrations at the start and end f the 5-day test: Bottle Initial DO (mg/L) Final DO (mg/L) Experimental 8.1 4.6 Blank 7.8 6.7 hat...
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