(A) You have a sample with an original concentration of 1.0 x 10^7 CFU/mL. With the...
(A) You have a sample with an original concentration of 1.0 x 10^7 CFU/mL. With the Plate Count Method, what final dilution factor would be needed to produce countable plates? Show your work. (B) Describe a dilution scheme (how many tubes, what volume in each tube, what DF is achieved in each step) that uses only the 9-mL blank diluent tubes to achieve the dilution needed for this FDF.
Homework Answers
CFU/ml = number of colonies/ dilution factor * volume plated
= 100/0.0001 * 0.1
100 * 10000 * 10
= 1*10^7 CFU/ml
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(A) You have a sample with an original concentration of 1.0 x
10^7 CFU/mL. With the...
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial cu...
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial culture 4 ml 9 ml 9ml 2ml 0.1 m 1 ml1 m0.1 m 400 CFU 400 CFU 140 CFU 16 CFU Individual tube dilutions Dilution Factor (-Total tube dilutions) Plated dilution factor 1. Explain why plates E and F have the same colony count. 2. Which plate is the countable plate? (30-300 countable range) Calculate the titer of the original culture in...
A bacterial sample has a concentration of 2 x106 CFUlml. Show a scheme of dilutions to...
A bacterial sample has a concentration of 2 x106 CFUlml. Show a scheme of dilutions to obtain 30-300 colonies on a plate. Your scheme should contain the volume of diluent (sterile water), volume of sample transferred each time, the concentration of bacteria (CFU/mL) in each dilution. You can assume you are plating 1 mL of the dilution on plates of nutrient agar. See Figure 1 in protocol 10. Dilution factor in single transfer (change x and y to numbers 10...
RESULTS For your dilution plates draw your results below. IMI Calculate the original CFU/ml in your...
RESULTS For your dilution plates draw your results below. IMI Calculate the original CFU/ml in your original broth tube, if you are within 500 CFU/ml of what the instructor calculated, you earn an extra credit point REVIEW QUESTIONS 1. A plate has 72 colonies with a total dilution factor of 10-7.0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?
please help me understand step by step how to figure out these problems. Equation 3: CFU/mL...
please help me understand step by step how to figure out these
problems.
Equation 3: CFU/mL = number of colonies/mL plated TDF of the culture used for plating For example, consider the sketch below. Using the information in the figure, the plate with 50 colonies would be chosen. 1.0 mL 1.0 mL 1.0 L 1.0 mL 1.0 mL A 0.1 ml de 2.0m00m 100 m 9.0 ml 9.0 ml 9.0 mi 9.9 ml 9.0 ml Original culture 1.0 ml 2.0...
help me with the math. m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or...
help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
.Viable plate count Using the formula: cfu/ml = (no. of colonies x dilution factor) / volume...
.Viable plate count Using the formula: cfu/ml = (no. of colonies x dilution factor) / volume of culture plate In plate 10-4 there were 150 colonies counted. Experimental process: Pipet 0.25 mL of the S. cerevisiae solution into the test tube labeled 10-1. Pipet the solution up and down several times to ensure all organisms are rinsed from the pipet into the test tube. Pipet 0.25 mL of the 10-1 S. cerevisiae solution into the test tube labeled 10-2. Pipet...
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1...
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1 ml 1 ml 1 ml CODILE 9ml 9ml 9ml 0.1 ml 0.1 ml colonies) (colonies colonies) Individual tube dilutions: BCD Dilution Factor: Total tube dilutions) IDE AB Plated dilution factor 1. Which plate is the countable plate? (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml.
please help with whatever possible. thank you so much in advance. Name One use of serial...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
You are given a culture that has a concentration of 2.1x10^9 cfu.ml. you are asked to...
You are given a culture that has a concentration of 2.1x10^9 cfu.ml. you are asked to dilute it and plate it so that you have 270 colonies on one plate. you are given plenty diluent but only 4 tubes with which to make your dilutions. Demonstrate how you would set up the dilution. For each step indicate in an orderly fashion the V2, V1, Diluent volume, dilution, c-dilution, DF, CDF up to that step and the concentration of each tube.
What inoculum is needed to get a CFU count of 1.4 x 10^8 CFU/ml when the...
What inoculum is needed to get a CFU count of 1.4 x 10^8 CFU/ml when the CFU count is 42, and the dilution factor is 10^-7?
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial culture 4 ml 9 ml 9ml 2ml 0.1 m 1 ml1 m0.1 m 400 CFU 400 CFU 140 CFU 16 CFU Individual tube dilutions Dilution Factor (-Total tube dilutions) Plated dilution factor 1. Explain why plates E and F have the same colony count. 2. Which plate is the countable plate? (30-300 countable range) Calculate the titer of the original culture in...
A bacterial sample has a concentration of 2 x106 CFUlml. Show a scheme of dilutions to obtain 30-300 colonies on a plate. Your scheme should contain the volume of diluent (sterile water), volume of sample transferred each time, the concentration of bacteria (CFU/mL) in each dilution. You can assume you are plating 1 mL of the dilution on plates of nutrient agar. See Figure 1 in protocol 10. Dilution factor in single transfer (change x and y to numbers 10...
RESULTS For your dilution plates draw your results below. IMI Calculate the original CFU/ml in your original broth tube, if you are within 500 CFU/ml of what the instructor calculated, you earn an extra credit point REVIEW QUESTIONS 1. A plate has 72 colonies with a total dilution factor of 10-7.0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?
please help me understand step by step how to figure out these
problems.
Equation 3: CFU/mL = number of colonies/mL plated TDF of the culture used for plating For example, consider the sketch below. Using the information in the figure, the plate with 50 colonies would be chosen. 1.0 mL 1.0 mL 1.0 L 1.0 mL 1.0 mL A 0.1 ml de 2.0m00m 100 m 9.0 ml 9.0 ml 9.0 mi 9.9 ml 9.0 ml Original culture 1.0 ml 2.0...
help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1 ml 1 ml 1 ml CODILE 9ml 9ml 9ml 0.1 ml 0.1 ml colonies) (colonies colonies) Individual tube dilutions: BCD Dilution Factor: Total tube dilutions) IDE AB Plated dilution factor 1. Which plate is the countable plate? (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml.
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
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