What inoculum is needed to get a CFU count of 1.4 x 10^8 CFU/ml when the...
What inoculum is needed to get a CFU count of 1.4 x 10^8 CFU/ml when the CFU count is 42, and the dilution factor is 10^-7?
Homework Answers
CFU per ml = number of colonies / (volume plated × dilution factor)
Volume plated = innoculum = ? = V
1.4 × 10^8 = 42 / (V × 10^-7)
V = 42 / (1.4 × 10^-7 × 10^8)
V = 3 ml
Innoculum needed is 3 ml.
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What inoculum is needed to get a CFU count of 1.4 x 10^8 CFU/ml
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Calculate the cfu/ml using the
formula
cfu/ml = # colonies x dilution factor
x plating factor (10)
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calculate the generation time (minutes/generation) of
4.9*10^6 CFUs/ml
calculate the generation time (minutes/ generation) of
155*10^8 CFUs/ml
for 3hrs 4.9*10^6
for 6hrs155*10^8
The students UR S pes at diferent times during the growth to do dilution plating and get viable plate counts. Here are the results for the samples taken at hours 3 and 6: 3 hours 10 dilution - too many to count 10"dilution - 476 CFUS 10% dilution - 49 CFUS 10 dilution = 5 CFUS 6 hours...
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