Calculate the cfu/ml using the formula
cfu/ml = # colonies x dilution factor x plating factor (10)
Homework Answers
1. CFU = 15 × 10^6 × 10 = 15 × 10^7 CFU per ml
2. CFU = 8 × 10^4 × 10 = 8 × 10^5 CFU per ml
3. CFU = 6 × 10^2 = 600 CFU per ml
Dilution factor = 1/dilution
So, if dilution is 1/100, then dilution factor will be 100.
Dilution factor = final volume / initial volume
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Calculate the cfu/ml using the
formula
cfu/ml = # colonies x dilution factor
x plating factor...
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c)You have 0.6 mL an undiluted culture at a density of 3.7 x 107 CFU/mL. You...
c)You have 0.6 mL an undiluted culture at a density of 3.7 x 107 CFU/mL. You add 5.4 mL of sterile diluent. What is the dilution and final cell density? e) Plating 2.0 mL of a sample diluted by a factor or 10-2 procedures 56 colonies. What was the original cell density in the sample? f)Plating 0.2 mL of a sample diluted by a factor or 10-4 procedures 87 colonies. What was the original cell density in the sample?
RESULTS For your dilution plates draw your results below. IMI Calculate the original CFU/ml in your...
RESULTS For your dilution plates draw your results below. IMI Calculate the original CFU/ml in your original broth tube, if you are within 500 CFU/ml of what the instructor calculated, you earn an extra credit point REVIEW QUESTIONS 1. A plate has 72 colonies with a total dilution factor of 10-7.0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?
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please help me understand step by step how to figure out these problems. Equation 3: CFU/mL...
please help me understand step by step how to figure out these
problems.
Equation 3: CFU/mL = number of colonies/mL plated TDF of the culture used for plating For example, consider the sketch below. Using the information in the figure, the plate with 50 colonies would be chosen. 1.0 mL 1.0 mL 1.0 L 1.0 mL 1.0 mL A 0.1 ml de 2.0m00m 100 m 9.0 ml 9.0 ml 9.0 mi 9.9 ml 9.0 ml Original culture 1.0 ml 2.0...
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Please help calculate average CFU/ml Treatment 1: BHI, 370c, +02 Time (min)oD (average) CFU/ml # of...
Please help calculate average CFU/ml
Treatment 1: BHI, 370c, +02 Time (min)oD (average) CFU/ml # of colonies 65, 118, 119, 13 8, 16, 13, o Dilution 0.11 E-6 E-7 15 30 0.12 0.19 E-6 E-7 97, 155, 213, 122 18, 8, 26, 10 45 60 0.31 0.51 E-6 E-7 224, 273, 156, 258 64, 44, 16, 66 75 90 0.88 52, 67, 96, TNTC 14, 5, 9, 93 0.98 E-7 E-8 105 120 1.07 E-7 E-8 400, 116, TNTC, 0...
calculate the generation time (minutes/generation) of 4.9*10^6 CFUs/ml calculate the generation time (minutes/ generation) of 155*10^8...
calculate the generation time (minutes/generation) of
4.9*10^6 CFUs/ml
calculate the generation time (minutes/ generation) of
155*10^8 CFUs/ml
for 3hrs 4.9*10^6
for 6hrs155*10^8
The students UR S pes at diferent times during the growth to do dilution plating and get viable plate counts. Here are the results for the samples taken at hours 3 and 6: 3 hours 10 dilution - too many to count 10"dilution - 476 CFUS 10% dilution - 49 CFUS 10 dilution = 5 CFUS 6 hours...
Write the formula for calculating the CFU/ml here and put in the numbers used. CFU (Colony Forming unit) CFU/ml= (Number of Colonies|Dilution factor) (Volume of culture plate) Conclusion: In a narrative form Using the 51 (Chapter 3, Cowan lecture book) Inoculation agar, Incubation, Isolation, Inspection, Identification Use the 5 l-s to explain how it was decided that the infectious southe was or was not the cause The number of colonies in first plate is 79. The number of colonies in...
RESULTS For your dilution plates draw your results below. IMI Calculate the original CFU/ml in your original broth tube, if you are within 500 CFU/ml of what the instructor calculated, you earn an extra credit point REVIEW QUESTIONS 1. A plate has 72 colonies with a total dilution factor of 10-7.0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?
please help me understand step by step how to figure out these
problems.
Equation 3: CFU/mL = number of colonies/mL plated TDF of the culture used for plating For example, consider the sketch below. Using the information in the figure, the plate with 50 colonies would be chosen. 1.0 mL 1.0 mL 1.0 L 1.0 mL 1.0 mL A 0.1 ml de 2.0m00m 100 m 9.0 ml 9.0 ml 9.0 mi 9.9 ml 9.0 ml Original culture 1.0 ml 2.0...
Please help calculate average CFU/ml
Treatment 1: BHI, 370c, +02 Time (min)oD (average) CFU/ml # of colonies 65, 118, 119, 13 8, 16, 13, o Dilution 0.11 E-6 E-7 15 30 0.12 0.19 E-6 E-7 97, 155, 213, 122 18, 8, 26, 10 45 60 0.31 0.51 E-6 E-7 224, 273, 156, 258 64, 44, 16, 66 75 90 0.88 52, 67, 96, TNTC 14, 5, 9, 93 0.98 E-7 E-8 105 120 1.07 E-7 E-8 400, 116, TNTC, 0...
calculate the generation time (minutes/generation) of
4.9*10^6 CFUs/ml
calculate the generation time (minutes/ generation) of
155*10^8 CFUs/ml
for 3hrs 4.9*10^6
for 6hrs155*10^8
The students UR S pes at diferent times during the growth to do dilution plating and get viable plate counts. Here are the results for the samples taken at hours 3 and 6: 3 hours 10 dilution - too many to count 10"dilution - 476 CFUS 10% dilution - 49 CFUS 10 dilution = 5 CFUS 6 hours...
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