What is the calculation of 960,000 CFU/ml on 1:1000 CFU count
Original cell count = 960,000 CFU per ml
Dilution = 1/1000
Final cell count = 960,000 × 1/1000 = 960 cells per ml
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What inoculum is needed to get a CFU count of 1.4 x 10^8 CFU/ml when the CFU count is 42, and the dilution factor is 10^-7?
Count the number of CFU from each of the plates (record TNTC if there are too many to count). Record your counts below and calculate the total number of bacteria per mL and the number of ampicillin-resistant bacteria per mL in the chicken fluid. Show all your calculations in a neat logical manner in the workspace on page 4. Plate Count Plates Without Ampicillin Dilution CFU/Plate Plate # 1 Plate # 2 Average/Plate CFU/mL of Fluid 10-4 52 80 66...
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Given the optical density of a sample culture how would you determine the CFU/ml without doing a standard plate count?
Calculate the cfu/ml using the formula cfu/ml = # colonies x dilution factor x plating factor (10) Temp. (OC) Organism #Colonies Dilution Staphylococcus aureus 43 15 1:1000000 51 1:10000 1:100 53 6
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial culture 4 ml 9 ml 9ml 2ml 0.1 m 1 ml1 m0.1 m 400 CFU 400 CFU 140 CFU 16 CFU Individual tube dilutions Dilution Factor (-Total tube dilutions) Plated dilution factor 1. Explain why plates E and F have the same colony count. 2. Which plate is the countable plate? (30-300 countable range) Calculate the titer of the original culture in...
9. A. You have diluted a sample by 1000 fold and plated 1 mL on an agar plate. You observe 35 colonies. What was the concentration of the original sample in CFU/mL?
The amount of 250 CFU per 0.01 ml is considered harmful to humans and other animals. If your sample contains close to or more than an excessive amount of bacterial contaminant, what can be done to decrease the numbers of bacteria without harming humans or animals (3pts). How would you know if your method of decreasing the numbers of this potentially harmful bacteria? * Hint* What needs to decrease? Total Count or Viable Count? Why?
Plating 1.0 ml of a sample diluted to 10^-4 produced 57 colonies. What was the CFU/ml in the original sample? What would it be if you plated 0.1 ml with same colonies formed?