Given the optical density of a sample culture how would you determine the CFU/ml without doing a standard plate count?

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Answer 1

Answer #1

1 OD of Ecoli has around 1*10⁹ cell per ml.

So we have E.coli cell and we know the OD then we can approximately calculate the number of cells present in the culture.

For other bacteria, we need to find the cells per OD and then we can use it to calculate to the number of cells.

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Answer 2

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