By total count you determine that the concentration of an organism in a liquid culture is ...
- By total count you determine that the concentration of an organism in a liquid culture is 5.6 x 107 CFU/ml and you have 1 ml of this culture. Assuming that total count equals viable count, design a practical dilution and plating scheme which will result in the formation of 280 colonies per plate. Include all steps and indicate volumes and dilutions.
Homework Answers
CFU/ml = number of colonies / dilution factor *volume plated in ml.
Colony = 280 per plate.
Volume plated = 0.1ml
Dilution factor = Need to find out
5.6*107 = 280 /Dilution factor *0.1
Dilution factor = 280/ 5.6*107 *0.1
= 280/560*104
= 1/2*104
= 5/105
= 5*10-5
So the required dilution factor is 5*10-5
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By total count you determine that the concentration of an
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