You are presented with the following results for a Standard Plate Count (SPC). What was the original starting concentration of the culture?
Homework Answers
In the standard Plate Count, we consider the plates with CFU of 30 to 300 only. This is because, 30 CFU will be too few and the plates with 300 will be too crowded that you wll not be able to identify individual colonies.
Concentration of the original culture is calculated this way:
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You are presented with the following results for a Standard
Plate Count (SPC). What was the...
A standard plate count of a culture of E. Coli has 175 colonies on the plate...
A standard plate count of a culture of E. Coli has 175 colonies on the plate labeled 10^-6 what is the original cell density (OCD, in CFU/ml) of the original culture?
you count 187 CFUS on a spread plate. The plate was prepared by spreading 0.2ml of...
you count 187 CFUS on a spread plate. The plate was prepared by spreading 0.2ml of a 1:100 dilution of a 1:50 dilution of a 1:10 dilution of the original sample. What is the concentration of the original sample. What is the concentration of the original culture in cells/ml
Count the number of CFU from each of the plates (record TNTC if there are too...
Count the number of CFU from each of the plates (record TNTC if there are too many to count). Record your counts below and calculate the total number of bacteria per mL and the number of ampicillin-resistant bacteria per mL in the chicken fluid. Show all your calculations in a neat logical manner in the workspace on page 4. Plate Count Plates Without Ampicillin Dilution CFU/Plate Plate # 1 Plate # 2 Average/Plate CFU/mL of Fluid 10-4 52 80 66...
Given the optical density of a sample culture how would you determine the CFU/ml without doing a standard plate count?
Given the optical density of a sample culture how would you determine the CFU/ml without doing a standard plate count?
please help with #3 and #4 3. Did you enumerate all the bact u you enumerate...
please help with #3 and #4
3. Did you enumerate all the bact u you enumerate all the bacteria present in the sample when you used the standard plate count method? Explain. 4. If you add 1.0 ml to 99 ml of water, what is the dilution of the sample? Results What is the number of colonies in each plate? 10-5 Culture 10-3 dilution plate 104 10-6 dilution plate dilution plate dilution plate Too numerous to 1848 - 384 1247...
may you help me figure out how to solve 7-9 Standard plate count. Include formulas and...
may you help me figure out how to solve 7-9
Standard plate count. Include formulas and calculations. Scientific notation. Show your work. UNITSI 7. Suppose you have 2.0 ml of an undiluted E.coli broth and need to begin your serial dilution by making a 10. solution. How much diluent would you need if you wanted to use the entire 2 ml of E. coli broth? 110-ml 102 = 2mL ol os dition 10 8. If you transfer 0.1 ml of...
What is the concentration of cells in the starting sample if 1 ml was plated per...
What is the concentration of cells in the starting sample if 1
ml was plated per dilution and the starting sample was river
water?
Report your answer below as a decimal with one significant digit
after the decimal and report the exponent the separate box. See
this example, if your answer is 5.2 x 108 you would
report it as "5.2"
You have the following results from a serial dilution experiment: Sample Dilution Plate Count 1:101 Too many to count...
please help me understand step by step how to figure out these problems. Equation 3: CFU/mL...
please help me understand step by step how to figure out these
problems.
Equation 3: CFU/mL = number of colonies/mL plated TDF of the culture used for plating For example, consider the sketch below. Using the information in the figure, the plate with 50 colonies would be chosen. 1.0 mL 1.0 mL 1.0 L 1.0 mL 1.0 mL A 0.1 ml de 2.0m00m 100 m 9.0 ml 9.0 ml 9.0 mi 9.9 ml 9.0 ml Original culture 1.0 ml 2.0...
RESULTS For your dilution plates draw your results below. IMI Calculate the original CFU/ml in your...
RESULTS For your dilution plates draw your results below. IMI Calculate the original CFU/ml in your original broth tube, if you are within 500 CFU/ml of what the instructor calculated, you earn an extra credit point REVIEW QUESTIONS 1. A plate has 72 colonies with a total dilution factor of 10-7.0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
please help with #3 and #4
3. Did you enumerate all the bact u you enumerate all the bacteria present in the sample when you used the standard plate count method? Explain. 4. If you add 1.0 ml to 99 ml of water, what is the dilution of the sample? Results What is the number of colonies in each plate? 10-5 Culture 10-3 dilution plate 104 10-6 dilution plate dilution plate dilution plate Too numerous to 1848 - 384 1247...
may you help me figure out how to solve 7-9
Standard plate count. Include formulas and calculations. Scientific notation. Show your work. UNITSI 7. Suppose you have 2.0 ml of an undiluted E.coli broth and need to begin your serial dilution by making a 10. solution. How much diluent would you need if you wanted to use the entire 2 ml of E. coli broth? 110-ml 102 = 2mL ol os dition 10 8. If you transfer 0.1 ml of...
What is the concentration of cells in the starting sample if 1
ml was plated per dilution and the starting sample was river
water?
Report your answer below as a decimal with one significant digit
after the decimal and report the exponent the separate box. See
this example, if your answer is 5.2 x 108 you would
report it as "5.2"
You have the following results from a serial dilution experiment: Sample Dilution Plate Count 1:101 Too many to count...
please help me understand step by step how to figure out these
problems.
Equation 3: CFU/mL = number of colonies/mL plated TDF of the culture used for plating For example, consider the sketch below. Using the information in the figure, the plate with 50 colonies would be chosen. 1.0 mL 1.0 mL 1.0 L 1.0 mL 1.0 mL A 0.1 ml de 2.0m00m 100 m 9.0 ml 9.0 ml 9.0 mi 9.9 ml 9.0 ml Original culture 1.0 ml 2.0...
RESULTS For your dilution plates draw your results below. IMI Calculate the original CFU/ml in your original broth tube, if you are within 500 CFU/ml of what the instructor calculated, you earn an extra credit point REVIEW QUESTIONS 1. A plate has 72 colonies with a total dilution factor of 10-7.0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?
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