A standard plate count of a culture of E. Coli has 175 colonies on the plate...

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A standard plate count of a culture of E. Coli has 175 colonies on the plate...

A standard plate count of a culture of E. Coli has 175 colonies on the plate labeled 10^-6 what is the original cell density (OCD, in CFU/ml) of the original culture?

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Answer 1

Answer #1

The label 10^-6 means that the original culture has been diluted 10⁶ times.

Since volume is not specified in the question it can be assumed that the volume of culture used to spread on the plate is 1 ml ( so that we can get the answer in CFU/ml). (CFU- colony forming units)

There are 175 colonies in 1 ml of culture that is been diluted 10⁶ times.
This means the original culture will have 10⁶ times the number of microbes (CFUs) in the diluted culture

So the original cell density is 175 X 10⁶ = 1.75 X 10⁸ CFU/ml

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Answer 2

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