Serial dilution problem You have a stock of 5% SDS. You take some of that stock...
Serial dilution problem
You have a stock of 5% SDS. You take some of that stock and dilute it 4-fold in tube #1.
the tubes hold 1.5ml. give an example of how much 5% stock and how much water you would use to make 4 fold dilution
- volume of 5% stock ?
- volume of water?
- How much volume transferred?
- if you took a sample out of tube 2 and did another 4 fold dilution, what concentration would there be in the tube 3?
Homework Answers
Answer:
Based on the given information:
- SDS Stock Concentration = 5%
- The stock was diluted 4-fold.Therefore concentration after dilution = 5% / 4 = 1.25%
- Volume tube#1 holds = 1.5ml
- Volume of SDS used = 1.5 ml / fold-dilution = 1.5ml / 4 = 0.375ml
- Volume of water used for dilution = 1.5ml - 0.375ml = 1.125ml
- How much volume transferred: Transfer 0.375ml volume of 5% SDS to 1.125ml of water in tube #1
Another 4 fold dilution:
- Concentration before 2nd dilution = 1.25%
- Concentration after 4-fold dilution = 1.25% / 4 = 0.3125%
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