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A student made an initial 1:5 dilution of protein lysate. Then 2mL of that was added...
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased...
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased as 25X TBE? 2) 2mL of a serum sample was added to 18mL of phosphate buffered saline (PBS) in Tube 1. 10mL of Tube 1 was added to 40mL of PBS in Tube What is the dilution of serum in Tube 2? 3) A student needed to prepare 500mL of 1X TAE buffer to run a QC gel. The stock solution in the lab...
7. What is the final dilution factor of a 4 fold serial dilution with 5 total tubes including the "neat" tu...
7. What is the final dilution factor of a 4 fold serial dilution with 5 total tubes including the "neat" tube? (neat means undiluted or the original specimen) 1:4 2 Neat ? Dilution factor
7. What is the final dilution factor of a 4 fold serial dilution with 5 total tubes including the "neat" tube? (neat means undiluted or the original specimen) 1:4 2 Neat ? Dilution factor
1) if 5 parts sample is added to 10 parts diluents, what is the dilution that...
1) if 5 parts sample is added to 10 parts diluents, what is the dilution that was just made? 2. What is the ratio if 5 parts sample volume is added to 10 parts diluents volume? 3. In a 1 to 3 dilution, what is the dilution FACTOR? 4. In a 1 to 10 dilution, what is the dilution FACTOR? 5. What is the dilution FACTOR if 40 mcL of sample added to 260 mcL of diluent? 6. What is...
Need the answers to all please :) 1) You recently purchased a new mitogen at 1mg/1ml...
Need the answers to all please :) 1) You recently purchased a new mitogen at 1mg/1ml (stock) and then prepared 1:100 dilutions as a working solution (tube A). The company also suggest verifying the optimal dilution for your experiment by doing serial (twofold) dilutions. Describe how you would prepare 500microliters of working solution (tube A). What volume of inhibitor will you add in microliter? 2) You would like to optimize the working concentration of the inhibitor, and therefore perform two-fold...
Serial dilution problem You have a stock of 5% SDS. You take some of that stock...
Serial dilution problem You have a stock of 5% SDS. You take some of that stock and dilute it 4-fold in tube #1. the tubes hold 1.5ml. give an example of how much 5% stock and how much water you would use to make 4 fold dilution - volume of 5% stock ? - volume of water? - How much volume transferred? - if you took a sample out of tube 2 and did another 4 fold dilution, what concentration...
1. Make a 10 fold serial dilution of a protein to achieve a final concentration of...
1. Make a 10 fold serial dilution of a protein to achieve a final concentration of 10ug/ml You are staring from a 10 mg/ml protein stock. Indicate the amount of sample needed (from previous tube) and the amount of H20 needed. Make your calculations for 100μ1 volume. (0.5pts) 10ul 90μ1 H20 10mg/ml lmg/ml
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine...
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine viable bacterial counts. Indicate the final dilutions in each of the two tubes. Note that two different volumes of the cells intube #2 are plated onto agar plates A and B. Indicate the dilution factor that will be used to report the original cell concentration on a per mL basis. 1 ml 1 ml Final dilution in Tube #1 = Final dilution in Tube...
If I transfer 120 uL from a microcentrifuge tube with an initial dilution factor of 2.2...
If I transfer 120 uL from a microcentrifuge tube with an initial dilution factor of 2.2 x 10-3 into a second tube containing 1.23mL of water what is my final dilution factor (accounting for both dilutions)? a.) 3.72 * 10-1 b.) 1.96 * 10^-4 c.) 3.02*10^-4 d.) 1.37*10^-1
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
Dilution, help?? 5. You add 25uls of antibody to 475 uls of water - what dilution...
Dilution, help??
5. You add 25uls of antibody to 475 uls of water - what dilution did you just make? 6. (3 pts) To obtain a countable plate to quantify your bacterial sample you first dilute the sample 1/500 in Tube 1 and then make a 1/25 dilution of this sample in Tube 2. If the total volume for your dilutions is 1 ml: a. How much water should be in Tube 1 and how much of your initial bacterial...
7. What is the final dilution factor of a 4 fold serial dilution with 5 total tubes including the "neat" tube? (neat means undiluted or the original specimen) 1:4 2 Neat ? Dilution factor
7. What is the final dilution factor of a 4 fold serial dilution with 5 total tubes including the "neat" tube? (neat means undiluted or the original specimen) 1:4 2 Neat ? Dilution factor
1. Make a 10 fold serial dilution of a protein to achieve a final concentration of 10ug/ml You are staring from a 10 mg/ml protein stock. Indicate the amount of sample needed (from previous tube) and the amount of H20 needed. Make your calculations for 100μ1 volume. (0.5pts) 10ul 90μ1 H20 10mg/ml lmg/ml
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine viable bacterial counts. Indicate the final dilutions in each of the two tubes. Note that two different volumes of the cells intube #2 are plated onto agar plates A and B. Indicate the dilution factor that will be used to report the original cell concentration on a per mL basis. 1 ml 1 ml Final dilution in Tube #1 = Final dilution in Tube...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
Dilution, help??
5. You add 25uls of antibody to 475 uls of water - what dilution did you just make? 6. (3 pts) To obtain a countable plate to quantify your bacterial sample you first dilute the sample 1/500 in Tube 1 and then make a 1/25 dilution of this sample in Tube 2. If the total volume for your dilutions is 1 ml: a. How much water should be in Tube 1 and how much of your initial bacterial...
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