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1. Make a 10 fold serial dilution of a protein to achieve a final concentration of 10ug/ml You are staring from a 10 mg/ml pr

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Answer #1

Initial stock concentration = 10 mg/mL
Final concentration = 10 ug/mL
Dilution factor = 10^-3

Final volume = 100 uL
Dilution 1: Take 10 uL of the stock and add it to 90 uL H2O; Final concentration = 1 mg/mL
Dilution 2: Take 10 uL of the dilution-1 and add it to 90 uL H2O; Final concentration = 0.1 mg/mL = 100 ug/mL
Dilution 3: Take 10 uL of the dilution-2 and add it to 90 uL H2O; Final concentration = 0.01 mg/mL = 10 ug/mL


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