Homework Answers
the dilutions will be as follows:
1:2.5, 1:2, 1:1.5, 1:1, 1:0.5, 1:0.25
Therefore, the final dilution of 1:0.25 will correspond to the final concentration of 0.02 µg/µL of diluted antibiotic.
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One component of the Minimum Inhibitory Concentration experiment is the creation of an antibiotic serial dilution....
Serial Dilution Questions 1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution...
Serial Dilution Questions
1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2,00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of...
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 3...
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2.00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of 100.00 mL. Calculate...
1. Make a 10 fold serial dilution of a protein to achieve a final concentration of...
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In lab you carried out a serial dilution and calculated the concentration of each solution. Starting...
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Lab Exercise 13: 1. Perform the following dilution calculation. If you have a sterile stock antibiotic...
Lab Exercise 13: 1. Perform the following dilution calculation. If you have a sterile stock antibiotic solution of 100 mg/mL, what volume of this stock should you add to 10 mL sterile broth to obtain a final concentration of 64 micrograms/mL? mL
Lab Exercise 13: 1. Perform the following dilution calculation. If you have a sterile stock antibiotic...
Lab Exercise 13: 1. Perform the following dilution calculation. If you have a sterile stock antibiotic solution of 100 mg/mL, what volume of this stock should you add to 10 mL sterile broth to obtain a final concentration of 64 micrograms/mL? mL
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
14. Analyze the following serial dilution experiment and answer the questions, each (a and b) worth...
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Electrolytes, Concentration and Dilution of Solutions n this part of the experiment, you will make a dilution of a stock solution of known concentration and calculate the concentration of the di...
Electrolytes, Concentration and Dilution of Solutions n this part of the experiment, you will make a dilution of a stock solution of known concentration and calculate the concentration of the dilute solution. DILUTION OF A STOCK SOLUTION PART C: Measurements and Observations Procedure Check out a 100-mL volumetric flask from your instructor. Fill test tube about 2/3 concentration of the active ingredient from its label including units. Record 1. orlt O " concentration of active ingredient. full of "Magic 2....
To dilute a bacterial culture, 100 mu l of a 16 hour culture is mixed with...
To dilute a bacterial culture, 100 mu l of a 16 hour culture is mixed with fresh culture media to a final volume of 5 ml. The 16 hour culture was diluted by a factor of: _____ An antibiotic (ampicillin) used during a Transformation experiment. Ampicillin is stored as a stock solution of 100 mg/ml. You are pouring plates from a 500 ml flask of Lauria Bertini agar (LB Agar) and you need an antibiotic concentration of 100 mu g/ml...
Serial Dilution Questions
1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2,00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of...
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2.00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of 100.00 mL. Calculate...
1. Make a 10 fold serial dilution of a protein to achieve a final concentration of 10ug/ml You are staring from a 10 mg/ml protein stock. Indicate the amount of sample needed (from previous tube) and the amount of H20 needed. Make your calculations for 100μ1 volume. (0.5pts) 10ul 90μ1 H20 10mg/ml lmg/ml
In lab you carried out a serial dilution and calculated the concentration of each solution. Starting with a 2.05 M HCl stock solution, three standard solutions are prepared by sequentially diluting 5.00 mL of each solution to 150.0 mL. What is the concentration of the final solution? 0.0683 M 2.28 times 10^-3 M 9.11 times 10^-6 M 7.59 times 10^-5 M 2.53 times 10^-6 M
Lab Exercise 13: 1. Perform the following dilution calculation. If you have a sterile stock antibiotic solution of 100 mg/mL, what volume of this stock should you add to 10 mL sterile broth to obtain a final concentration of 64 micrograms/mL? mL
Lab Exercise 13: 1. Perform the following dilution calculation. If you have a sterile stock antibiotic solution of 100 mg/mL, what volume of this stock should you add to 10 mL sterile broth to obtain a final concentration of 64 micrograms/mL? mL
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
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To dilute a bacterial culture, 100 mu l of a 16 hour culture is mixed with fresh culture media to a final volume of 5 ml. The 16 hour culture was diluted by a factor of: _____ An antibiotic (ampicillin) used during a Transformation experiment. Ampicillin is stored as a stock solution of 100 mg/ml. You are pouring plates from a 500 ml flask of Lauria Bertini agar (LB Agar) and you need an antibiotic concentration of 100 mu g/ml...
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