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Lab Exercise 13: 1. Perform the following dilution calculation. If you have a sterile stock antibiotic...
Lab Exercise 13: 1. Perform the following dilution calculation. If you have a sterile stock antibiotic...
Lab Exercise 13: 1. Perform the following dilution calculation. If you have a sterile stock antibiotic solution of 100 mg/mL, what volume of this stock should you add to 10 mL sterile broth to obtain a final concentration of 64 micrograms/mL? mL
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
One component of the Minimum Inhibitory Concentration experiment is the creation of an antibiotic serial dilution....
One component of the Minimum Inhibitory Concentration experiment is the creation of an antibiotic serial dilution. In this assignment, you are to determine the appropriate dilution from the original stock needed to reach the final concentration of antibiotic in 1 mL of media. In your experiment, you are to add only 10 mu L of antibiotic to the media. You are given the starting concentration of the antibiotic (the stock solution) and the final concentrations needed. The first three answers...
Serial Dilution Questions 1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution...
Serial Dilution Questions
1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2,00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of...
plote a graph Lab Exercise 4B Example: Calculation of C, for Tube #2 ? Concentration of...
plote a graph
Lab Exercise 4B Example: Calculation of C, for Tube #2 ? Concentration of maltese solution (C) 2.5mg/ml. Volume of standard solution (V.) - 200 ml 0.2 ml Concentration of maltose solution after dilution (C) Volume of maltose solution after dilution (V.) = 100 ml -0.05 mg/ml C 2.5 mg/ml X 0.2 100 ml Absorbund at 540 Tube 0.0 b. 554 HE Volume of Volume of water concentration added to make standard maltose of maltose number solution (L)...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
Electrolytes, Concentration and Dilution of Solutions n this part of the experiment, you will make a dilution of a stock solution of known concentration and calculate the concentration of the di...
Electrolytes, Concentration and Dilution of Solutions n this part of the experiment, you will make a dilution of a stock solution of known concentration and calculate the concentration of the dilute solution. DILUTION OF A STOCK SOLUTION PART C: Measurements and Observations Procedure Check out a 100-mL volumetric flask from your instructor. Fill test tube about 2/3 concentration of the active ingredient from its label including units. Record 1. orlt O " concentration of active ingredient. full of "Magic 2....
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 3...
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2.00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of 100.00 mL. Calculate...
You have a stock solution that contains 24 mg/ml of protein. You want to make a...
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il Repeat Proble... ... Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein, You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you...
Lab Exercise 13: 1. Perform the following dilution calculation. If you have a sterile stock antibiotic solution of 100 mg/mL, what volume of this stock should you add to 10 mL sterile broth to obtain a final concentration of 64 micrograms/mL? mL
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
One component of the Minimum Inhibitory Concentration experiment is the creation of an antibiotic serial dilution. In this assignment, you are to determine the appropriate dilution from the original stock needed to reach the final concentration of antibiotic in 1 mL of media. In your experiment, you are to add only 10 mu L of antibiotic to the media. You are given the starting concentration of the antibiotic (the stock solution) and the final concentrations needed. The first three answers...
Serial Dilution Questions
1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2,00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of...
plote a graph
Lab Exercise 4B Example: Calculation of C, for Tube #2 ? Concentration of maltese solution (C) 2.5mg/ml. Volume of standard solution (V.) - 200 ml 0.2 ml Concentration of maltose solution after dilution (C) Volume of maltose solution after dilution (V.) = 100 ml -0.05 mg/ml C 2.5 mg/ml X 0.2 100 ml Absorbund at 540 Tube 0.0 b. 554 HE Volume of Volume of water concentration added to make standard maltose of maltose number solution (L)...
Electrolytes, Concentration and Dilution of Solutions n this part of the experiment, you will make a dilution of a stock solution of known concentration and calculate the concentration of the dilute solution. DILUTION OF A STOCK SOLUTION PART C: Measurements and Observations Procedure Check out a 100-mL volumetric flask from your instructor. Fill test tube about 2/3 concentration of the active ingredient from its label including units. Record 1. orlt O " concentration of active ingredient. full of "Magic 2....
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2.00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of 100.00 mL. Calculate...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il Repeat Proble... ... Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein, You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you...
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