a) If a compound has the wavelength of maximum absorbance of 600 nm, what would happen...
a) If a compound has the wavelength of maximum absorbance of 600 nm, what would happen if all of the samples were measured at a slightly higher wavelength (i.e. 620 nm)?
b) When plotting data of concentration vs. absorption of the compound, will the change in wavelength affect the R^2 value of the graph? why or why not?
c) Will the change in wavelength affect the molar absorptivity calculated from the graph? why or why not?
Homework Answers
a) Has been uploaded in the picture.
b) The R2 value for any particular compound is always constant and hence it has no effect when thee there is any change in wavelength.
c) The Molar Absorptivity Constant is specific for every single solution, and at every wavelength. When you are taking an absorbance spectrum, and measuring the absorbance at different wavelengths, this is the only factor that is changing, as the concentration of the solution remains the same, and so does the path length.
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a) If a compound has the wavelength of maximum absorbance of 600
nm, what would happen...
2. The absorbance of a 2.31 x 10 a wavelength of 266 nm in a 1.00...
2. The absorbance of a 2.31 x 10 a wavelength of 266 nm in a 1.00 cm cell. absorptivity at 266 nm. M solution of a compound is 0.822 at Calculate the molar The following absorption data were calculated in-an experiment: 3. Absorbance 0.000 0.090 0.181 0.271 0.361 0.450 0.539 0.627 0.7 Concentration (м) 0.000 1x10-3 2x10-3 3x10 3 4x10-3 5x10~3 6x10-3 7x10-3 8x1 Plot Absorbance vs. Concentration and calculate the molar absorptivity coefficient from the slope if the a)...
6) According to the Beer-Lambert Law, what would happen to the absorbance of a solution if...
6) According to the Beer-Lambert Law, what would happen to the absorbance of a solution if the concentration of analyte (substance being studied) is tripled? 7) At a wavelength of 447 nm, the absorbance of a solution that is 5X10M in the FeSCN complex is found to be 0.981. Assuming this is accurate, calculate the molar absorption coefficient (absorptivity) for the complex if the optical path length is 1.0 cm.
2. The absorbance of a 2.31 % 10 M solution of a compound is 0.822 at...
2. The absorbance of a 2.31 % 10 M solution of a compound is 0.822 at a wavelength of 266 nm in a 1.00 cm cell. Calculate the molar absorptivity at 266 nm. The following absorption data were calculated in an experiment: Absorbance 0.000 0.00 0.181 0.271 0.361 0.450 0.539 0.627 0.7 Concentration 0.000 1x10-3 2x10-3 3x10-3 4x10- 5x10- 6x10 3 7x10- 8x1 (M) Plot Absorbance vs. Concentration and a) calculate the molar absorptivity coefficient from the slope if the...
Why is it appropriate to connect the data points for graph 1 (absorbance vs wavelength) but...
Why is it appropriate to connect the data points for graph 1
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Absorbance vs Wavelength 0.55 0.50 0.45 0.40 0.15 0.30 Absorbance 0.25 0.20 0.15 0.10 . 0.00 380 400 4200 460 480 500 520 540 560 580 600 620 640 660 680 700 720 700 750 780 Wavelength Absorbance vs Concentration 0.45 y = 0.002 x 0.002 R=0.9997 0.40...
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You have a compound that has an absorption at 291 nm. At a concentration of 0.025...
You have a compound that has an absorption at 291 nm. At a
concentration of 0.025 mM, the absorbance A291 = 0.40.
What is its molar absorptivity of this sample (in
M-1cm-1)? Assume you are using a 1 cm cuvette
for your experiment.
Incorrect Question 1 0/2 pts You have a compound that has an absorption at 291 nm. At a concentration of 0.025 mM, the absorbance A291 = 0.40. What is its molar absorbtivity of this sample (in M?cm-?)?...
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2. The absorbance of a 2.31 x 10 a wavelength of 266 nm in a 1.00 cm cell. absorptivity at 266 nm. M solution of a compound is 0.822 at Calculate the molar The following absorption data were calculated in-an experiment: 3. Absorbance 0.000 0.090 0.181 0.271 0.361 0.450 0.539 0.627 0.7 Concentration (м) 0.000 1x10-3 2x10-3 3x10 3 4x10-3 5x10~3 6x10-3 7x10-3 8x1 Plot Absorbance vs. Concentration and calculate the molar absorptivity coefficient from the slope if the a)...
6) According to the Beer-Lambert Law, what would happen to the absorbance of a solution if the concentration of analyte (substance being studied) is tripled? 7) At a wavelength of 447 nm, the absorbance of a solution that is 5X10M in the FeSCN complex is found to be 0.981. Assuming this is accurate, calculate the molar absorption coefficient (absorptivity) for the complex if the optical path length is 1.0 cm.
2. The absorbance of a 2.31 % 10 M solution of a compound is 0.822 at a wavelength of 266 nm in a 1.00 cm cell. Calculate the molar absorptivity at 266 nm. The following absorption data were calculated in an experiment: Absorbance 0.000 0.00 0.181 0.271 0.361 0.450 0.539 0.627 0.7 Concentration 0.000 1x10-3 2x10-3 3x10-3 4x10- 5x10- 6x10 3 7x10- 8x1 (M) Plot Absorbance vs. Concentration and a) calculate the molar absorptivity coefficient from the slope if the...
Why is it appropriate to connect the data points for graph 1
(absorbance vs wavelength) but not appropriate for graph 2
(absorbance vs concentration)? What type of information does each
graph convey?
Absorbance vs Wavelength 0.55 0.50 0.45 0.40 0.15 0.30 Absorbance 0.25 0.20 0.15 0.10 . 0.00 380 400 4200 460 480 500 520 540 560 580 600 620 640 660 680 700 720 700 750 780 Wavelength Absorbance vs Concentration 0.45 y = 0.002 x 0.002 R=0.9997 0.40...
Absorbance of chlorophyll a -Rate of photosynthesis 400 500 600 700 Wavelength of light (nm) The graph above shows the absorption spectrum for chlorophyll a. According to the data, which wavelength of light is most effective in driving photosynthesis? A. 420 nm B. 575 nm C.625 nm D.730 nm
You have a compound that has an absorption at 291 nm. At a
concentration of 0.025 mM, the absorbance A291 = 0.40.
What is its molar absorptivity of this sample (in
M-1cm-1)? Assume you are using a 1 cm cuvette
for your experiment.
Incorrect Question 1 0/2 pts You have a compound that has an absorption at 291 nm. At a concentration of 0.025 mM, the absorbance A291 = 0.40. What is its molar absorbtivity of this sample (in M?cm-?)?...
A 5.90 x 10-5 M sample of compound X in a solvent has an absorbance of 0.344 at 528 nm in a 1.000 cm cuvet. The solvent alone has an absorbance of 0.056. What is the molar absorptivity of compound X? € = M-1.cm-1 A different sample of compound X in the same solvent has an absorbance of 0.506 at 528 nm when measured with the same cuvet. What is the concentration of this second sample? [X] = mol X
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