You have a compound that has an absorption at 291 nm. At a concentration of 0.025 mM, the absorbance A291 = 0.40. What is its molar absorptivity of this sample (in M-1cm-1)? Assume you are using a 1 cm cuvette for your experiment.
Homework Answers
molar absorbtivity = absorbance / (concentration × path length)
Given, absorbance = 0.40, concentration = 0.025 mM = 0.025×10^-3 M and path length = 1cm
Thus
0.40 / (0.025×10^-3 × 1) = 16000 M^-1 cm^-1
Thus your answer to type is 16000.
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