You have isolated an unknown colored compound (MW = 200,000 g/mol) with a concentration of 5 mg/mL. After you add 2 mL of your unknown to 8 mL of buffer, you obtain an absorption spectrum of your colored compound. You record an Absorbance value of 0.57 at its lambda max, 460 nm. The light path is 1 cm.
B. Calculate the extinction coefficient (M-1cm-1) of your diluted colored compound. Show your work.
You have isolated an unknown colored compound (MW = 200,000 g/mol) with a concentration of 5...
You have isolated an unknown colored compound (MW = 200,000 g/mol) with a concentration of 5 mg/mL. After you add 2 mL of your unknown to 8 mL of buffer, you obtain an absorption spectrum of your colored compound. You record an Absorbance value of 0.57 at its lambda max, 460 nm. The light path is 1 cm. Calculate the molar concentration (M) of your diluted sample. Show your work. B. Calculate the extinction coefficient (M-1cm-1) of your diluted...
Calculate the extinction coefficient of PNP in M‒1cm‒1 (p-nitrophenol MW 140 g/mol) if 0.1 g sample is prepared in 500 mL buffer, the above solution produced an absorbance of 4.5 when using a spectrophotometer with a cuvette path length of l = 0.50 cm.
An unknown amount of a compound with a molecular mass of 284.04 g/mol is dissolved in a 10 mL volumetric flask. A 1.00 mL aliquot of this solution is transferred to a 25 mL volumetric flask, and enough water is added to dilute to the mark. The absorbance of this diluted solution at 347 nm is 0.472 in a 1.000 cm cuvet. The molar absorptivity for this compound at 347 nm is ?347=6023 M−1cm−1. What is the concentration of the...
An unknown amount of a compound with a molecular mass of 274.11 g/mol 274.11 g/mol is dissolved in a 10 mL volumetric flask. A 1.00 mL aliquot of this solution is transferred to a 25 mL volumetric flask, and enough water is added to dilute to the mark. The absorbance of this diluted solution at 325 nm 325 nm is 0.523 0.523 in a 1.000 cm cuvet. The molar absorptivity for this compound at 325 nm 325 nm is ?...
An unknown amount of a compound with a molecular mass of 270.93 g/mol is dissolved in a 10-mL volumetric flask. A 1.00-mL aliquot of this solution is transferred to a 25-mL volumetric flask and e water is added to dilute to the mark. The absorbance of this diluted solution at 339 nm is 0.517 in a 1 cm cuvette. The molar absorptivity for this compound at 339 nm is esso- 5835 M-1cm-1 (a) What is the concentration of the compound...
You have a compound that has an absorption at 291 nm. At a
concentration of 0.025 mM, the absorbance A291 = 0.40.
What is its molar absorptivity of this sample (in
M-1cm-1)? Assume you are using a 1 cm cuvette
for your experiment.
Incorrect Question 1 0/2 pts You have a compound that has an absorption at 291 nm. At a concentration of 0.025 mM, the absorbance A291 = 0.40. What is its molar absorbtivity of this sample (in M?cm-?)?...
An unknown amount of a compound with a molecular mass of 294.46 g/mol is dissolved in a 10 mL volumetric flask. A 1.00 mL aliquot of this solution is transferred to a 25 mL volumetric flask, and enough water is added to dilute to the mark. The absorbance of this diluted solution at 343 nm is 0.456 in a 1.000 cm cuvette. The molar absorptivity for this compound at 343 nm is €343 = 5721 M-?cm-1. What is the concentration...
If 2.0 mL of distilled water is added to 3.0 mL of a 0.150 M solution of CoCly, what is the concentration of the resulting solution? Show your work. (a) What is a calibration curve? (b) Why do we use a calibration curve to determine the concentration of our unknown 5. A colored sample with a concentration of 0.160 M has an absorbance of 0.80 in a 1.0 cm path length cuvette. (a) What would be the absorbance expected if...
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Suppose you obtained the following data from two standard solutions of known concentrations using a UV-Vis spectroscope: Analysis # Conc. (mg/L) 8.00 20.00 Absorbance (at 255 nm) 0.160 0.390 What is the concentration (in mg/L) of an unknown solution with an absorbance of 0.200 at 255 nm 10 points)? The unknown solution contains the same analyte as the standard solution does (Assume a path length of 1cm). 10. You prepared a stock solution by dissolving a...
Questions 1. Consider the calibration graph and the absorption spectrum for solutions of metal ion, M * Absorption Spectrum for M Calibration Curve for M 0.8 0.7 1.2 0.6 1 0.5 S 0.8 0.4 0.6 0.3 0.4 0.2 0.1 0.2 0- 0 0.5 0.4 0.3 0.2 0.1 400 450 500 550 600 650 700 [M*1 (Molar) (入) nm a) At what wavelength was the calibration curve obtained? Explain your answer. b) Use the calibration curve to calculate the concentration (M)...