An unknown amount of a compound with a molecular mass of 274.11 g/mol 274.11 g/mol is dissolved in a 10 mL volumetric flask. A 1.00 mL aliquot of this solution is transferred to a 25 mL volumetric flask, and enough water is added to dilute to the mark. The absorbance of this diluted solution at 325 nm 325 nm is 0.523 0.523 in a 1.000 cm cuvet. The molar absorptivity for this compound at 325 nm 325 nm is ? 325 =6421 M −1 cm −1 . ϵ325=6421 M−1cm−1. What is the concentration of the compound in the cuvet?
Chemistry
The Molar Mass of the compound= , absorbance =0.523, Molar absorptivity
Using Beer-Lambert's law, we get -
,
where, A= Absorbance, - Molar Absorptivity, c-concentration of the solution in the cuvette, l- length of the cuvette=1 cm.
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An unknown amount of a compound with a molecular mass of 274.11 g/mol 274.11 g/mol is...
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