This last set of questions builds on the previous series. You have purified an enzyme catalyzing...
You are a summer intern in a clinical hematology lab. The lab director gives you a sample of a patient’s blood proteins and asks you to characterize the thrombin in the sample. She also tells you that thrombin is a serine protease important in blood clotting (see Table 5.3), and this patient is a newborn with uncontrolled bleeding. To characterize the thrombin in the sample, you must remove two proteins that interfere with the thrombin activity assay: cytochrome c and...