Question

Life requires energy. Use what you know about the energy transformations that occur in animal cells to discuss some of the energy processing that is currently ongoing in your cells. Include the role of ATP and enzymes in your discussion.

Answer 1

Cells manage a wide range of functions in their tiny package — growing, moving, housekeeping, and so on — and most of those functions require energy. Cells seek their energy in the form of food molecules. Cellular nutrients come in many forms, including sugars and fats. In order to provide a cell with energy, these molecules have to pass across the cell membrane, which functions as a barrier — but not an impassable one. Like the exterior walls of a house, the plasma membrane is semi-permeable. In much the same way that doors and windows allow necessities to enter the house, various proteins that span the cell membrane permit specific molecules into the cell, although they may require some energy input to accomplish this task.

Complex organic food molecules such as sugars, fats, and proteins are rich sources of energy for cells because much of the energy used to form these molecules is literally stored within the chemical bonds that hold them together. Scientists can measure the amount of energy stored in foods using a device called a bomb calorimeter. With this technique, food is placed inside the calorimeter and heated until it burns. The excess heat released by the reaction is directly proportional to the amount of energy contained in the food.

In reality, of course, cells don't work quite like calorimeters. Rather than burning all their energy in one large reaction, cells release the energy stored in their food molecules through a series of oxidation reactions. Oxidation describes a type of chemical reaction in which electrons are transferred from one molecule to another, changing the composition and energy content of both the donor and acceptor molecules. Food molecules act as electron donors. During each oxidation reaction involved in food breakdown, the product of the reaction has a lower energy content than the donor molecule that preceded it in the pathway. At the same time, electron acceptor molecules capture some of the energy lost from the food molecule during each oxidation reaction and store it for later use. Eventually, when the carbon atoms from a complex organic food molecule are fully oxidized at the end of the reaction chain, they are released as waste in the form of carbon dioxide.

Cells do not use the energy from oxidation reactions as soon as it is released. Instead, they convert it into small, energy-rich molecules such as ATP and nicotinamide adenine dinucleotide (NADH), which can be used throughout the cell to power metabolism and construct new cellular components. In addition, workhorse proteins called enzymes use this chemical energy to catalyze, or accelerate, chemical reactions within the cell that would otherwise proceed very slowly. Enzymes do not force a reaction to proceed if it wouldn't do so without the catalyst; rather, they simply lower the energy barrier required for the reaction to begin.

The particular energy pathway that a cell employs depends in large part on whether that cell is a eukaryote or a prokaryote. Eukaryotic cells use three major processes to transform the energy held in the chemical bonds of food molecules into more readily usable forms — often energy-rich carrier molecules. Adenosine 5'-triphosphate, or ATP, is the most abundant energy carrier molecule in cells. This molecule is made of a nitrogen base (adenine), a ribose sugar, and three phosphate groups. The word adenosine refers to the adenine plus the ribose sugar. The bond between the second and third phosphates is a high-energy bond.

The first process in the eukaryotic energy pathway is glycolysis, which literally means "sugar splitting." During glycolysis, single molecules of glucose are split and ultimately converted into two molecules of a substance called pyruvate; because each glucose contains six carbon atoms, each resulting pyruvate contains just three carbons. Glycolysis is actually a series of ten chemical reactions that requires the input of two ATP molecules. This input is used to generate four new ATP molecules, which means that glycolysis results in a net gain of two ATPs. Two NADH molecules are also produced; these molecules serve as electron carriers for other biochemical reactions in the cell.

Glycolysis is an ancient, major ATP-producing pathway that occurs in almost all cells, eukaryotes and prokaryotes alike. This process, which is also known as fermentation, takes place in the cytoplasm and does not require oxygen. However, the fate of the pyruvate produced during glycolysis depends upon whether oxygen is present. In the absence of oxygen, the pyruvate cannot be completely oxidized to carbon dioxide, so various intermediate products result. For example, when oxygen levels are low, skeletal muscle cells rely on glycolysis to meet their intense energy requirements. This reliance on glycolysis results in the buildup of an intermediate known as lactic acid, which can cause a person's muscles to feel as if they are "on fire." Similarly, yeast, which is a single-celled eukaryote, produces alcohol (instead of carbon dioxide) in oxygen-deficient settings.

In contrast, when oxygen is available, the pyruvates produced by glycolysis become the input for the next portion of the eukaryotic energy pathway. During this stage, each pyruvate molecule in the cytoplasm enters the mitochondrion, where it is converted into acetyl CoA, a two-carbon energy carrier, and its third carbon combines with oxygen and is released as carbon dioxide. At the same time, an NADH carrier is also generated. Acetyl CoA then enters a pathway called the citric acid cycle, which is the second major energy process used by cells. The eight-step citric acid cycle generates three more NADH molecules and two other carrier molecules: FADH₂ and GTP.

The third major process in the eukaryotic energy pathway involves an electron transport chain, catalyzed by several protein complexes located in the mitochondrional inner membrane. This process, called oxidative phosphorylation, transfers electrons from NADH and FADH₂ through the membrane protein complexes, and ultimately to oxygen, where they combine to form water. As electrons travel through the protein complexes in the chain, a gradient of hydrogen ions, or protons, forms across the mitochondrial membrane. Cells harness the energy of this proton gradient to create three additional ATP molecules for every electron that travels along the chain. Overall, the combination of the citric acid cycle and oxidative phosphorylation yields much more energy than fermentation - 15 times as much energy per glucose molecule! Together, these processes that occur inside the mitochondion, the citric acid cycle and oxidative phosphorylation, are referred to as respiration, a term used for processes that couple the uptake of oxygen and the production of carbon dioxide.

The electron transport chain in the mitochondrial membrane is not the only one that generates energy in living cells. In plant and other photosynthetic cells, chloroplasts also have an electron transport chain that harvests solar energy. Even though they do not contain mithcondria or chloroplatss, prokaryotes have other kinds of energy-yielding electron transport chains within their plasma membranes that also generate energy.

When energy is abundant, eukaryotic cells make larger, energy-rich molecules to store their excess energy. The resulting sugars and fats — in other words, polysaccharides and lipids — are then held in reservoirs within the cells, some of which are large enough to be visible in electron micrographs.

Animal cells can also synthesize branched polymers of glucose known as glycogen, which in turn aggregate into particles that are observable via electron microscopy. A cell can rapidly mobilize these particles whenever it needs quick energy. Athletes who "carbo-load" by eating pasta the night before a competition are trying to increase their glycogen reserves. Under normal circumstances, though, humans store just enough glycogen to provide a day's worth of energy. Plant cells don't produce glycogen but instead make different glucose polymers known as starches, which they store in granules.

In addition, both plant and animal cells store energy by shunting glucose into fat synthesis pathways. One gram of fat contains nearly six times the energy of the same amount of glycogen, but the energy from fat is less readily available than that from glycogen. Still, each storage mechanism is important because cells need both quick and long-term energy depots. Fats are stored in droplets in the cytoplasm; adipose cells are specialized for this type of storage because they contain unusually large fat droplets. Humans generally store enough fat to supply their cells with several weeks' worth of energy.

Cells need energy to accomplish the tasks of life. Beginning with energy sources obtained from their environment in the form of sunlight and organic food molecules, eukaryotic cells make energy-rich molecules like ATP and NADH via energy pathways including photosynthesis, glycolysis, the citric acid cycle, and oxidative phosphorylation. Any excess energy is then stored in larger, energy-rich molecules such as polysaccharides (starch and glycogen) and lipids.