We have seen all term that cells use the hydrolysis of high energy phosphate from ATP...
We have seen all term that cells use the hydrolysis of high energy phosphate from ATP to make metabolic reactions thermodynamically favorable. Whereas most enzymes that utilize ATP hydrolyze between the b and g phosphates (yielding ADP + Pi), some enzymes hydrolyze ATP between the a and b phosphates (yielding AMP and PPi). ∆G°’ of phosphate hydrolysis is -31 kJ/mol for ATP + H2O --> ADP + Pi, and ∆G°’ of phosphate hydrolysis is -46.5 kJ/mol for ATP + H2O --> AMP + PPi. In this problem you will compare how much free energy is released under cellular conditions when ATP is cleaved between the b and g phosphates vs between the a and b phosphates. Assume that the cellular concentrations of Pi, ATP, ADP, AMP and PPi are 40 mM, 4.0 mM, 0.40 mM and 40 µM, 3.0 µM, respectively.
(a) Calculate the ∆G’ for phosphate hydrolysis of the g phosphate of ATP.
(b) Calculate the ∆G’ for phosphate hydrolysis between the a and b phosphates of ATP. Compare the value to that calculated in part (a), and comment on why some enzymes might hydrolyze ATP between the a and b phosphates.
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We have seen all term that cells use the hydrolysis of high
energy phosphate from ATP...
please help! thanks TABLE 12-4 Standard Free Energy Change for Phosphate Hydrolysis Compound AGⓇ' (kJ. mol-')...
please help! thanks
TABLE 12-4 Standard Free Energy Change for Phosphate Hydrolysis Compound AGⓇ' (kJ. mol-') Phosphoenolpyruvate -61.9 1,3-Bisphosphoglycerate -49.4 ATP → AMP + PP, -45.6 Phosphocreatine -43.1 ATP ADP + P -30.5 Glucose-1-phosphate -20.9 PP, 2P, -19.2 Glucose-6-phosphate -13.8 Glycerol-3-phosphate -9.2 thioester bond AGⓇ' = -31.5 kJ•mol-1 H20 O CH3 -C-s-CoA I CH3 -C-0- + COA-SH Acetyl-CoA The formation of acetyl CoA from acetate is an ATP driven reaction: Acetate + ATP + COA <--> acetyl COA + AMP...
The formation of glucose-1-phosphate from glucose and orthophosphate has a G of + 20.9 kJ/mol. If...
The formation of glucose-1-phosphate from glucose and orthophosphate has a G of + 20.9 kJ/mol. If this reaction were coupled to another reaction in order to produce an exergonic reaction, which of the following would produce an overall endergonic reaction and therefore not be suitable for coupling? A) creatine phosphate + H2O creatine + Pi B) AMP + H2O adenosine + Pi C) ATP + H2O AMP + PPi D) ATP + H2O ADP + Pi...
Please help with this question about Gibb's free energy. The answer is: -47.6 kJ/mol. Please show...
Please help with this question about Gibb's free energy. The
answer is: -47.6 kJ/mol. Please show steps on how to find this
answer!
1) In a rat hepatocyte, the concentrations of ATP, ADP and Pi are 3.4 mM, 1.3 mM and 4.8 mM, respectively. ATP + H2O → ADP + Pi + H+ a) Calculate the actual Gibb's free energy change for hydrolysis of ATP in this cell. ([H2O) = 1M; [H+] = 1M; R = 8.315 JK-mol-1; T =...
The value of the standard free energy (∆G°’) for the hydrolysis of ATP (ATP + H2O -> ADP + Pi)...
The value of the standard free energy (∆G°’) for the hydrolysis of ATP (ATP + H2O -> ADP + Pi) is relatively difficult to determine because of the small concentration of ATP remaining at equilibrium. The value can be determined from the equilibrium constant of two related chemical reactions. Glucose-6-phosphate + H2O -> glucose + Pi ; k’eq = 270 ATP + glucose -> ADP + glucose-6-phosphate ; K’eq = 890 From this information, calculate the standard free energy of...
Biochemistry What is the AG for the hydrolysis of ATP under cellular conditions where: [ATP] =...
Biochemistry
What is the AG for the hydrolysis of ATP under cellular conditions where: [ATP] = 3 mm [ADP] = 1 mM [Pi] = 5 mM А -30.5 kJ/mol B -35.0 kJ/mol с -44.0 kJ/mol D -47.0 kJ/mol E -50.0 kJ/mol
Shown is acetyl phosphate. Say a new enzyme uses acetyl phosphate to phosphorylate ADP to make ATP (acetyl phosphate + ADP —> acetate + ATP). What is the standard free energy change of this reacti...
Shown is acetyl phosphate. Say a new enzyme uses acetyl
phosphate to phosphorylate ADP to make ATP (acetyl phosphate + ADP
—> acetate + ATP). What is the standard free energy change of
this reaction?
CH3 Table 13-2 Standard Free Energies of Phosphate Hydrolysis of Some Compounds of Biological Interest AGo (kJ mol 1) 61.9 -49.4 45.6 43.1 -43.1 30.5 -20.9 19.2 13.8 13.8 -9.2 Compound Phosphoenolpyruvate 1,3-Bisphosphoglycerate ATP AMP + PP,) Acetyl phosphate Phosphocreatine ATP ㈠ ADP + Pi)...
Consider the hydrolysis of ATP: ATP(aq) + H2O(l) → ADP(aq) + Pi(aq). This reaction has ΔH°=...
Consider the hydrolysis of ATP: ATP(aq) + H2O(l) → ADP(aq) + Pi(aq). This reaction has ΔH°= −24.3 kJ/mol and ΔS°= +21.6 J/mol-K. The actual concentrations of ATP, ADP, and Pi are not 1 M in a biological cell. How much energy can the conversion of ATP to ADP supply when it occurs at physiological conditions in E. coli where the temperature is 37°C and the approximate concentrations are ATP = 11.2 mM, ADP = 1.52 mM, Pi = 20.0 mM?...
the enzyme hexokinase can catalyze the following reaction: fructose + ATP -> fructose 6 phosphate +...
the enzyme hexokinase can catalyze the following
reaction:
fructose + ATP -> fructose 6 phosphate + adp
if the deltaG of hydrolysis of fructose 6 phosphate to
fructose + Pi is -13.8 kj/mol and hydrolysis of atp to adp + pi is
-30kj/mol, calculate Keq for the reaction above (R = 8.31 j mol-1
K-1, T = 298 k
3a. The enzyme hexokinase can catalyze the following reaction: Fructose + ATP → Fructose-6-phosphate + ADP If the ΔGo of hydrolysis...
Since AMP can also be hydrolyzed to release energy, why do cells bother to use ATP...
Since AMP can also be hydrolyzed to release energy, why do cells bother to use ATP (which requires the cell to have more phosphate) to power cellular reactions? Question 1 options: a. ATP fits better into enzymes. b. ATP hydrolysis releases more energy than AMP hydrolysis. c. ATP stores for a longer time than AMP. d. Cells have more than enough phosphate, and it serves few roles anyway.
The protein Src is a signaling molecule that is phosphorylated by acetyl phosphate and dephosphorylated by...
The protein Src is a signaling molecule that is phosphorylated
by acetyl phosphate and dephosphorylated by hydrolysis. Thus, Src
catalyzes the acetyl-phosphate hydrolysis reaction:
PICTURE. Please answer all the problems and explain.
Exam 2: Practice problems Chem4400 1. The protein Sre is a signaling molecule that is phosphory lated by acetyl phosphate and dephosphorylated by hydrolysis. Thus, Sre catalyzes the acetyl-phosphate hydrolysis reaction: (1) CH3CO-Pi (aq) + H20 (l)-→ CH3COOH (aq) + P (a) AG--40. Ik/ / mol As with...
please help! thanks
TABLE 12-4 Standard Free Energy Change for Phosphate Hydrolysis Compound AGⓇ' (kJ. mol-') Phosphoenolpyruvate -61.9 1,3-Bisphosphoglycerate -49.4 ATP → AMP + PP, -45.6 Phosphocreatine -43.1 ATP ADP + P -30.5 Glucose-1-phosphate -20.9 PP, 2P, -19.2 Glucose-6-phosphate -13.8 Glycerol-3-phosphate -9.2 thioester bond AGⓇ' = -31.5 kJ•mol-1 H20 O CH3 -C-s-CoA I CH3 -C-0- + COA-SH Acetyl-CoA The formation of acetyl CoA from acetate is an ATP driven reaction: Acetate + ATP + COA <--> acetyl COA + AMP...
Please help with this question about Gibb's free energy. The
answer is: -47.6 kJ/mol. Please show steps on how to find this
answer!
1) In a rat hepatocyte, the concentrations of ATP, ADP and Pi are 3.4 mM, 1.3 mM and 4.8 mM, respectively. ATP + H2O → ADP + Pi + H+ a) Calculate the actual Gibb's free energy change for hydrolysis of ATP in this cell. ([H2O) = 1M; [H+] = 1M; R = 8.315 JK-mol-1; T =...
Biochemistry
What is the AG for the hydrolysis of ATP under cellular conditions where: [ATP] = 3 mm [ADP] = 1 mM [Pi] = 5 mM А -30.5 kJ/mol B -35.0 kJ/mol с -44.0 kJ/mol D -47.0 kJ/mol E -50.0 kJ/mol
Shown is acetyl phosphate. Say a new enzyme uses acetyl
phosphate to phosphorylate ADP to make ATP (acetyl phosphate + ADP
—> acetate + ATP). What is the standard free energy change of
this reaction?
CH3 Table 13-2 Standard Free Energies of Phosphate Hydrolysis of Some Compounds of Biological Interest AGo (kJ mol 1) 61.9 -49.4 45.6 43.1 -43.1 30.5 -20.9 19.2 13.8 13.8 -9.2 Compound Phosphoenolpyruvate 1,3-Bisphosphoglycerate ATP AMP + PP,) Acetyl phosphate Phosphocreatine ATP ㈠ ADP + Pi)...
the enzyme hexokinase can catalyze the following
reaction:
fructose + ATP -> fructose 6 phosphate + adp
if the deltaG of hydrolysis of fructose 6 phosphate to
fructose + Pi is -13.8 kj/mol and hydrolysis of atp to adp + pi is
-30kj/mol, calculate Keq for the reaction above (R = 8.31 j mol-1
K-1, T = 298 k
3a. The enzyme hexokinase can catalyze the following reaction: Fructose + ATP → Fructose-6-phosphate + ADP If the ΔGo of hydrolysis...
The protein Src is a signaling molecule that is phosphorylated
by acetyl phosphate and dephosphorylated by hydrolysis. Thus, Src
catalyzes the acetyl-phosphate hydrolysis reaction:
PICTURE. Please answer all the problems and explain.
Exam 2: Practice problems Chem4400 1. The protein Sre is a signaling molecule that is phosphory lated by acetyl phosphate and dephosphorylated by hydrolysis. Thus, Sre catalyzes the acetyl-phosphate hydrolysis reaction: (1) CH3CO-Pi (aq) + H20 (l)-→ CH3COOH (aq) + P (a) AG--40. Ik/ / mol As with...
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