![Exam 2: Practice problems Chem4400 1. The protein Sre is a signaling molecule that is phosphory lated by acetyl phosphate and dephosphorylated by hydrolysis. Thus, Sre catalyzes the acetyl-phosphate hydrolysis reaction: (1) CH3CO-Pi (aq) + H20 (l)-→ CH3COOH (aq) + P (a) AG--40. Ik/ / mol As with many biochemical reactions, phosphorylation is driven by ATP hydrolysis: (2) ATP(aq) + H2O (/) → ADP (aq) + P, (ag) Cellular concentrations of the relevant species are: [ATP] [ADP] [Pl 1 mM; [CHsCO-P] 0.1 mM: [CH3COOH-10.0 mM. You can assume that the activity coefficients are 1 . (a) Write an expression of K for reaction I. (b) Calculate ΔG, for reaction 1 at cellular concentrations, 25°C, pH 7.0, and 1 bar. (c) Calculate ΔG, for reaction 2 at the same conditions. Can reaction 2 spontaneously drive the reverse of reaction 1? (d) Even though the hydrolysis of ATP drives many biochemical reactions, the phosphate bond is not a thermodynamically anomalous bond. Give 2 reasons why the ATP hydrolysis reaction is such a favorable reaction.](http://img.homeworklib.com/questions/206cd3b0-a6e3-11ea-97e1-91f5cee52b09.png?x-oss-process=image/resize,w_560)
The protein Src is a signaling molecule that is phosphorylated by acetyl phosphate and dephosphorylated by hydrolysis. Thus, Src catalyzes the acetyl-phosphate hydrolysis reaction:
PICTURE. Please answer all the problems and explain.
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(d)
Reason 1 : - products of hydrolysis (ADP +Pi) are more stablethan reactant(ATP) due to the presence of resonance stabilization.which means ADP have more resonance forms than ATP.
Reason 2 :- ADP molecule don't have as many negative charge in close proximity as ATP and due to loss of one inorganic phosphate,ADP is better solvated by water.
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The protein Src is a signaling molecule that is phosphorylated
by acetyl phosphate and dephosphorylated by...
5.The following reaction has a ΔG°'--61.9 kJ/mol at 25°C, Phosphoenolpyruvate (PEP) +H2O Pyruvate + Inorganic Phosphate...
5.The following reaction has a ΔG°'--61.9 kJ/mol at 25°C, Phosphoenolpyruvate (PEP) +H2O Pyruvate + Inorganic Phosphate (Pi) (a) Is the reaction favorable under standard biochemical conditions? Explain your answer. (b) What is the equilibrium constant (K) of this reaction at 25°C? In cells the direct hydrolysis of PEP rarely occurs. Rather, the hydrolysis of PEP and the reverse hydrolysis of ATP are coupled (c) w rite out the coupled reaction and determine its ΔG at 25°C The hydrolysis of ATP...
The enzyme hexokinase catalyzes the following reaction: Glucose + ATP ↔ Glucose 6-phosphate + ADP For...
The enzyme hexokinase catalyzes the following reaction: Glucose + ATP ↔ Glucose 6-phosphate + ADP For this reaction, ΔG° = +16.7 kJ/mol a. Calculate the equilibrium constant for this reaction at 25 °C. Is this reaction spontaneous under standard conditions at this temperature? Calculate the ΔG if the reaction temperature was 25 °C and the concentrations are: glucose, 55.0 mM,; ATP, 5.00 mM; ADP, 1.00 mM; and glucose 6-Phosphate, 0.100 mM. Would the reaction be spontaneous under the conditions of...
Shown is acetyl phosphate. Say a new enzyme uses acetyl phosphate to phosphorylate ADP to make ATP (acetyl phosphate + ADP —> acetate + ATP). What is the standard free energy change of this reacti...
Shown is acetyl phosphate. Say a new enzyme uses acetyl
phosphate to phosphorylate ADP to make ATP (acetyl phosphate + ADP
—> acetate + ATP). What is the standard free energy change of
this reaction?
CH3 Table 13-2 Standard Free Energies of Phosphate Hydrolysis of Some Compounds of Biological Interest AGo (kJ mol 1) 61.9 -49.4 45.6 43.1 -43.1 30.5 -20.9 19.2 13.8 13.8 -9.2 Compound Phosphoenolpyruvate 1,3-Bisphosphoglycerate ATP AMP + PP,) Acetyl phosphate Phosphocreatine ATP ㈠ ADP + Pi)...
We have seen all term that cells use the hydrolysis of high energy phosphate from ATP...
We have seen all term that cells use the hydrolysis of high energy phosphate from ATP to make metabolic reactions thermodynamically favorable. Whereas most enzymes that utilize ATP hydrolyze between the b and g phosphates (yielding ADP + Pi), some enzymes hydrolyze ATP between the a and b phosphates (yielding AMP and PPi). ∆G°’ of phosphate hydrolysis is -31 kJ/mol for ATP + H2O --> ADP + Pi, and ∆G°’ of phosphate hydrolysis is -46.5 kJ/mol for ATP + H2O...
2. For the following reaction: ATP+ creatine + H2O creatine phosphate + ADP + P, The...
2. For the following reaction: ATP+ creatine + H2O creatine phosphate + ADP + P, The relevant half reactions are: ATP → ADP + Pi Creatine phosphate + H2O-> creatine AG-30.5 kJ/mol ΔG°,--43.1 kJ/mol a. (2 pts.) Calculate the ΔG°, for the overall reaction. b. (2 pts.) Is the reaction spontaneous or non-spontaneous? Endergonic or exergonic?
The common molecule produced from all foods at the second stage of catabolism is A) ADP. B acetyl...
just checking my answers, except for number 10.
The common molecule produced from all foods at the second stage of catabolism is A) ADP. B acetyl-CoA. C) glucose. D) citric acid. Which group of compounds includes only low-energy molecules? 1. 2. Hih A)ATP; acetyl-CoA; NADH; FADH2 C) ADP: CO2; NADH; FADH2 DONO BADP: acetyl-CoA; NAD+:FAD-mau ADP: CO2: NAD+, FAD After O2 has been reduced in the electron transport chain, the oxygen atoms are part of what molecules? A) ATP 3....
CHEM 351 Biochemistry I - Fall 2018 Study Problems for Metabolism and Glucose Catabolism For the...
CHEM 351 Biochemistry I - Fall 2018 Study Problems for Metabolism and Glucose Catabolism For the reaction: aA + bB =cC + dD Gibbs free energy: AG = AG" + RT IN THE glucose + glucose 6-phosphate + H20 1. The equilibrium constant for phosphorylation of glucose is: Ke = 3.97 x 10- a. Calculate the standard Gibbs free energy for the uncatalyzed reaction. b. Use the physiological concentrations for glucose (2.0 mm) and inorganic phosphate (20 mM) to determine...
need help understanding why it is B and not D Mit lilall during the day. Usylithesis...
need help understanding why it is B and not D
Mit lilall during the day. Usylithesis produces more oxygen than is consumed by respiration during the day. 2. A researcher claims that the synthesis of ATP from ADP and inorganic phosphate (P.) is essential to cellular function. Which of the following statements best helps justify the researcher's claim? A. ADP is a small molecule that some cells release into their environment as a way of communicating with other cells. ATP...
2. The first step in glycolysis is catalyzed by the enzyme herokinase: glucose + ATP glucose-6-P...
2. The first step in glycolysis is catalyzed by the enzyme herokinase: glucose + ATP glucose-6-P + ADP This reaction can be thought of as two coupled reactions (P is phosphate): (1) glucose + P + glucose-6-P (2) ATP + ADP + P For the overall reaction, AG" = -16.7 kJ/mol. For the hydrolysis of ATP (equation 2), AG = -310 kJ/mol. All reactions are performed at 37°C. (a) What is AGº for reaction 1? Iglucose - 6-11 if AG...
To answer this question, please reference the Problem Solving Video: Free Energy, ATP, and Creatine in...
To answer this question, please reference the Problem Solving Video: Free Energy, ATP, and Creatine in Resting Muscles. The standard free energy, AGⓇ', value for an enzymatic reaction with several al components is the sum of the AGⓇ values for each component. Creatine kinase catalyzes the transfer of a phosphate group from creatine phosphate to ADP to form ATP. Standard Free Energy of Hydrolysis Standard E Compound kJ.mol-1 creatine phosphate -43.1 ATP (to ADP) -30.5 creatine phosphate +H,0 creatine +...
5.The following reaction has a ΔG°'--61.9 kJ/mol at 25°C, Phosphoenolpyruvate (PEP) +H2O Pyruvate + Inorganic Phosphate (Pi) (a) Is the reaction favorable under standard biochemical conditions? Explain your answer. (b) What is the equilibrium constant (K) of this reaction at 25°C? In cells the direct hydrolysis of PEP rarely occurs. Rather, the hydrolysis of PEP and the reverse hydrolysis of ATP are coupled (c) w rite out the coupled reaction and determine its ΔG at 25°C The hydrolysis of ATP...
Shown is acetyl phosphate. Say a new enzyme uses acetyl
phosphate to phosphorylate ADP to make ATP (acetyl phosphate + ADP
—> acetate + ATP). What is the standard free energy change of
this reaction?
CH3 Table 13-2 Standard Free Energies of Phosphate Hydrolysis of Some Compounds of Biological Interest AGo (kJ mol 1) 61.9 -49.4 45.6 43.1 -43.1 30.5 -20.9 19.2 13.8 13.8 -9.2 Compound Phosphoenolpyruvate 1,3-Bisphosphoglycerate ATP AMP + PP,) Acetyl phosphate Phosphocreatine ATP ㈠ ADP + Pi)...
2. For the following reaction: ATP+ creatine + H2O creatine phosphate + ADP + P, The relevant half reactions are: ATP → ADP + Pi Creatine phosphate + H2O-> creatine AG-30.5 kJ/mol ΔG°,--43.1 kJ/mol a. (2 pts.) Calculate the ΔG°, for the overall reaction. b. (2 pts.) Is the reaction spontaneous or non-spontaneous? Endergonic or exergonic?
just checking my answers, except for number 10.
The common molecule produced from all foods at the second stage of catabolism is A) ADP. B acetyl-CoA. C) glucose. D) citric acid. Which group of compounds includes only low-energy molecules? 1. 2. Hih A)ATP; acetyl-CoA; NADH; FADH2 C) ADP: CO2; NADH; FADH2 DONO BADP: acetyl-CoA; NAD+:FAD-mau ADP: CO2: NAD+, FAD After O2 has been reduced in the electron transport chain, the oxygen atoms are part of what molecules? A) ATP 3....
CHEM 351 Biochemistry I - Fall 2018 Study Problems for Metabolism and Glucose Catabolism For the reaction: aA + bB =cC + dD Gibbs free energy: AG = AG" + RT IN THE glucose + glucose 6-phosphate + H20 1. The equilibrium constant for phosphorylation of glucose is: Ke = 3.97 x 10- a. Calculate the standard Gibbs free energy for the uncatalyzed reaction. b. Use the physiological concentrations for glucose (2.0 mm) and inorganic phosphate (20 mM) to determine...
need help understanding why it is B and not D
Mit lilall during the day. Usylithesis produces more oxygen than is consumed by respiration during the day. 2. A researcher claims that the synthesis of ATP from ADP and inorganic phosphate (P.) is essential to cellular function. Which of the following statements best helps justify the researcher's claim? A. ADP is a small molecule that some cells release into their environment as a way of communicating with other cells. ATP...
2. The first step in glycolysis is catalyzed by the enzyme herokinase: glucose + ATP glucose-6-P + ADP This reaction can be thought of as two coupled reactions (P is phosphate): (1) glucose + P + glucose-6-P (2) ATP + ADP + P For the overall reaction, AG" = -16.7 kJ/mol. For the hydrolysis of ATP (equation 2), AG = -310 kJ/mol. All reactions are performed at 37°C. (a) What is AGº for reaction 1? Iglucose - 6-11 if AG...
To answer this question, please reference the Problem Solving Video: Free Energy, ATP, and Creatine in Resting Muscles. The standard free energy, AGⓇ', value for an enzymatic reaction with several al components is the sum of the AGⓇ values for each component. Creatine kinase catalyzes the transfer of a phosphate group from creatine phosphate to ADP to form ATP. Standard Free Energy of Hydrolysis Standard E Compound kJ.mol-1 creatine phosphate -43.1 ATP (to ADP) -30.5 creatine phosphate +H,0 creatine +...
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