Calculate the extinction coefficient of PNP in M‒1cm‒1 (p-nitrophenol MW 140 g/mol) if 0.1 g sample...
Calculate the extinction coefficient of PNP in M‒1cm‒1 (p-nitrophenol MW 140 g/mol) if 0.1 g sample is prepared in 500 mL buffer, the above solution produced an absorbance of 4.5 when using a spectrophotometer with a cuvette path length of l = 0.50 cm.
Homework Answers
Add Answer to:
Calculate the extinction coefficient of PNP in M‒1cm‒1
(p-nitrophenol MW 140 g/mol) if 0.1 g sample...
In an 'Enzyme Kinetics' experiment, the following calibration data, product (p-nitrophenol, PNP) concentration (M) versus UV...
In an 'Enzyme Kinetics' experiment, the following calibration data, product (p-nitrophenol, PNP) concentration (M) versus UV Absorbance 405 nm, was obtained If the UV absorbance 405 nm within the 1.5 mL-cuvette was measured as 0.144 in one of the trials during the experiment, determine the amount of pNP (in mg) produced. Assume total volume of reaction mixture stays at 1.5 mL at all times. (Round up to two decimal places) Molecular Weight of PNP: 139.11 g/mol PNP Concentration UV Absorbancem...
Extinction coefficient for NADH at 340 nm = 6220 M-1cm-1 What is the theoretical absorbance at...
Extinction coefficient for NADH at 340 nm = 6220 M-1cm-1
What is the theoretical absorbance at 340 nm of a 0.01 M solutions NADH, assuming a 1-cm pathlength? What dilution would be necessary to get the absorbance from Question 1 down to 3.1? ( -_ - mL of 0.1 M NADH to - mL H20)
You have isolated an unknown colored compound (MW = 200,000 g/mol) with a concentration of 5...
You have isolated an unknown colored compound (MW = 200,000 g/mol) with a concentration of 5 mg/mL. After you add 2 mL of your unknown to 8 mL of buffer, you obtain an absorption spectrum of your colored compound. You record an Absorbance value of 0.57 at its lambda max, 460 nm. The light path is 1 cm. Calculate the molar concentration (M) of your diluted sample. Show your work. B. Calculate the extinction coefficient (M-1cm-1) of your diluted...
You have isolated an unknown colored compound (MW = 200,000 g/mol) with a concentration of 5...
You have isolated an unknown colored compound (MW = 200,000 g/mol) with a concentration of 5 mg/mL. After you add 2 mL of your unknown to 8 mL of buffer, you obtain an absorption spectrum of your colored compound. You record an Absorbance value of 0.57 at its lambda max, 460 nm. The light path is 1 cm. Calculate the molar concentration (M) of your diluted sample. Show your work. B. Calculate the extinction coefficient (M-1cm-1) of your diluted...
Please answer both 1. Often when handling proteins, we use concentration in terms of mg/ml. A...
Please answer both 1. Often when handling proteins, we use concentration in terms of mg/ml. A high concentration is greater than 1 mg/ml and low concentrations are below 0.1 mg/ml (roughly). If you obtained a sample of MDH that was 10 micromoles/L, what is the concentration in mg/ml? Assume that the molecular weight for MDH is 32 kDa. 2. Using Beer's Law, determine the concentration of your sample if the UV-vis reading at 280 nm was 1.2 absorbance units and...
Given the fact that the molar extinction coefficient at 470 nm is 6800 cm-1 M-1 and...
Given the fact that the molar extinction coefficient at 470 nm is 6800 cm-1 M-1 and the radius of the 4-dram vial is 1.00 cm, what would be the absorbance for the highest standard solution (25.00 mL) and for the lowest standard (5.00 mL)? Preparation of iron standard solution (0.01 mg/mL): Weigh (to the nearest 0.1 mg) 0.0350 g of reagent grade (NH4)2Fe(SO4)2•6 H2O into a 500-mL volumetric flask. Dissolve in 250 mL of water that contains ~1 mL of...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FDSC Blue Dye #1 solution of unknown concentration was 0.229. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express your answer in 10" (if your answer is 5.52x10-M then...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FD&C Blue Dye #1 solution of unknown concentration was 0.229. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express your answer in 10-5M (if your answer is 5.52x105M then...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FD&C Blue Dye #1 solution of unknown concentration was 0.212. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express your answer in 10-SM (if your answer is 5.52x10-SM then...
1.Calculate the number of moles of avidin added for each titration based on the added volume...
1.Calculate the number of moles of avidin added for each titration based on the added volume of avidin stock solution and a MW of 68,000 g/mol. Conduct the following steps for each titration: 1) Add Vbuffer of 0.02 M sodium phosphate buffer, pH 7, to a disposable plastic cuvette. 2) Add Vavidin of a solution of 10 mg/mL avidin in 0.02 M sodium phosphate buffer, pH 7. Mix thoroughly by micropipetting up and down. 3) Put the cuvette in the...
In an 'Enzyme Kinetics' experiment, the following calibration data, product (p-nitrophenol, PNP) concentration (M) versus UV Absorbance 405 nm, was obtained If the UV absorbance 405 nm within the 1.5 mL-cuvette was measured as 0.144 in one of the trials during the experiment, determine the amount of pNP (in mg) produced. Assume total volume of reaction mixture stays at 1.5 mL at all times. (Round up to two decimal places) Molecular Weight of PNP: 139.11 g/mol PNP Concentration UV Absorbancem...
Extinction coefficient for NADH at 340 nm = 6220 M-1cm-1
What is the theoretical absorbance at 340 nm of a 0.01 M solutions NADH, assuming a 1-cm pathlength? What dilution would be necessary to get the absorbance from Question 1 down to 3.1? ( -_ - mL of 0.1 M NADH to - mL H20)
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FDSC Blue Dye #1 solution of unknown concentration was 0.229. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express your answer in 10" (if your answer is 5.52x10-M then...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FD&C Blue Dye #1 solution of unknown concentration was 0.229. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express your answer in 10-5M (if your answer is 5.52x105M then...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FD&C Blue Dye #1 solution of unknown concentration was 0.212. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express your answer in 10-SM (if your answer is 5.52x10-SM then...
Most questions answered within 3 hours.
-
The Problem: The Case of the Harmonizing Vacations
Your CEO is exploring partnering with a European...
asked 43 minutes ago -
A chemical equation is balanced by adding coefficients in front
of some formulas so that the...
asked 42 minutes ago -
From the literature (reference your sources): What are the
lattice parameters of calcite and aragonite? Why...
asked 1 hour ago -
Your system is rejecting the question am asking which is
preceded by a case study. It...
asked 1 hour ago -
3. On January 2, 2000, Larry creates a trust with himself as
trustee. Larry as trustee...
asked 1 hour ago -
A member of the volleyball team spikes the ball. During this
process, she changes the velocity...
asked 1 hour ago -
Are adult gamers less likely to use a gaming console (Xbox,
PlayStation, Wii, etc...) than teen...
asked 2 hours ago -
The University of
Texas recently reported that 43% of college students aged 18-24
would spend their...
asked 2 hours ago -
The length of stay at a specific emergency department in
Phoenix, Arizona, in 2009 had a...
asked 1 hour ago -
. Please give the mechanism for this type of problem. Step by
Step
The toxin that...
asked 1 hour ago -
If you have a 1M stock solution and you want to dilute 1 :10
with water,...
asked 1 hour ago -
In a load instruction, the effective address is obtained by
A) Retriving the address from a...
asked 1 hour ago