Given the fact that the molar extinction coefficient at 470 nm is 6800 cm-1 M-1 and...
Given the fact that the molar extinction coefficient at 470 nm is 6800 cm-1 M-1 and the radius of the 4-dram vial is 1.00 cm, what would be the absorbance for the highest standard solution (25.00 mL) and for the lowest standard (5.00 mL)?
Preparation of iron standard solution (0.01 mg/mL): Weigh (to the nearest 0.1 mg) 0.0350 g of reagent grade (NH4)2Fe(SO4)2•6 H2O into a 500-mL volumetric flask. Dissolve in 250 mL of water that contains ~1 mL of 98 % H2SO4 before diluting to the mark with water and mix very well. The stock solution was diluted by transferring 25mL stock into a 100mL volumetric flask and filled to volume, and the same for 5mL of stock solution.
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Given the fact that the molar extinction coefficient at 470 nm
is 6800 cm-1 M-1 and...
Please type in the Computer so I can be able to read it. A student need...
Please type in the Computer so I can be able to read it.
A student need to prepare four standard solutions by serially diluting a stock solution with concentration 5.0 times 10^-4 N. Standard I will be created by transferring 5.00 mL of the stock solution to the mark with deionized water. The remaining standard solutions (Standards 2 - 4) will be created by transferring 25.00 mL of the previously made standard solution to a 50.00 mL volumetric flask, then...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FDSC Blue Dye #1 solution of unknown concentration was 0.229. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express your answer in 10" (if your answer is 5.52x10-M then...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FD&C Blue Dye #1 solution of unknown concentration was 0.229. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express your answer in 10-5M (if your answer is 5.52x105M then...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FD&C Blue Dye #1 solution of unknown concentration was 0.212. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express your answer in 10-SM (if your answer is 5.52x10-SM then...
Human myoglobin is a protein with a molar extinction coefficient at 280nm of 1.40 x 104...
Human myoglobin is a protein with a molar extinction coefficient at 280nm of 1.40 x 104 M-1cm-1 . 100.0 uL of the stock solution of myoglobin (the tube was marked as 0.050 mg/mL) was mixed with 0.900 mL of buffer, giving an absorbance at 280nm of 0.810 (cuvette is 1.00 cm pathlength). From this information, what is the molecular weight of human myoglobin? Do you think this is a reasonable value? Explain WHY or WHY NOT?
A standard solution of iron was made by weighing 0.075 g of Fe(NH4)2(SO4)2 6(H2O) in 250...
A standard solution of iron was made by weighing 0.075 g of Fe(NH4)2(SO4)2 6(H2O) in 250 mL. Aliquots of this standard solution (see below) were transferred to a 100 mL volumetric flask, pH adjusted with citrate, and reacted with hydroquinone and o-phenanthroline and diluted to volume (100 mL). The absorbance was measured in a 1.0-cm cell using a Genesys 20 Spectrophotometer at 508 nm: Aliquot of standard solution Absorbance 1.0 mL 0.079 2.0 mL 0.163 5.0 mL 0.413 10.0 mL...
THE DETERMINATION OF IRON BY SPECTROPHOTOMETRY INTRODUCTION In this experiment, the red-orange colored complex formed between...
THE DETERMINATION OF IRON BY SPECTROPHOTOMETRY INTRODUCTION In this experiment, the red-orange colored complex formed between iron(II) and 1,10- phenanthroline (Eqn.) is used in determination of iron by spectrophotometry. Fe+3PhenH Fe(Phen)2 +3H red-orange (A 512 nm) An excess of reducing reagent, such as hydroxylamine or hydroquinone, is often used to reduce and maintain iron in +2 oxidation state. The complex, once formed, is very stable, and can be stored for a long time. Required Reading: Skoog and West (9E): Chapter...
1.1 Accurately (using an analytical balance ONLY) weigh 1.4 g of Fe(NH4)2(SO4) 2-6H20 1.2 Dissolve in...
1.1 Accurately (using an analytical balance ONLY) weigh 1.4 g of Fe(NH4)2(SO4) 2-6H20 1.2 Dissolve in 50 mL of 4% sylturisacid in a beaker. 1.3 Transfer into a 1 L volumetric flask; adjust the volume and mix well. Note the purity of the Fe(NH4)2(SO4)2.6H20 given on the label of the container. 1.4 Transfer this solution into a 1 L glass bottle. Preparation of working iron standard solution: 1.5 Pipet 10.00 mL of the iron standard stock solution into a 500.0...
The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist...
The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FD&C Blue Dye #1 solution of unknown concentration was 0.295. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express the answer in micromolar (notice micro means 10-6) Concentration of Blue Dye (M) Absorbance...
The absorption coefficient of bovine immunoglobulin at 280 nm is 210,000 M-1 cm-1 . what is...
The absorption coefficient of bovine immunoglobulin at 280 nm is 210,000 M-1 cm-1 . what is the absorbance of a 0.1 mg ml-1 solution across a 1 cm path? what percentage of the incident light is transmitted by this solution? mw (150,000 D)
Please type in the Computer so I can be able to read it.
A student need to prepare four standard solutions by serially diluting a stock solution with concentration 5.0 times 10^-4 N. Standard I will be created by transferring 5.00 mL of the stock solution to the mark with deionized water. The remaining standard solutions (Standards 2 - 4) will be created by transferring 25.00 mL of the previously made standard solution to a 50.00 mL volumetric flask, then...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FDSC Blue Dye #1 solution of unknown concentration was 0.229. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express your answer in 10" (if your answer is 5.52x10-M then...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FD&C Blue Dye #1 solution of unknown concentration was 0.229. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express your answer in 10-5M (if your answer is 5.52x105M then...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FD&C Blue Dye #1 solution of unknown concentration was 0.212. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express your answer in 10-SM (if your answer is 5.52x10-SM then...
THE DETERMINATION OF IRON BY SPECTROPHOTOMETRY INTRODUCTION In this experiment, the red-orange colored complex formed between iron(II) and 1,10- phenanthroline (Eqn.) is used in determination of iron by spectrophotometry. Fe+3PhenH Fe(Phen)2 +3H red-orange (A 512 nm) An excess of reducing reagent, such as hydroxylamine or hydroquinone, is often used to reduce and maintain iron in +2 oxidation state. The complex, once formed, is very stable, and can be stored for a long time. Required Reading: Skoog and West (9E): Chapter...
1.1 Accurately (using an analytical balance ONLY) weigh 1.4 g of Fe(NH4)2(SO4) 2-6H20 1.2 Dissolve in 50 mL of 4% sylturisacid in a beaker. 1.3 Transfer into a 1 L volumetric flask; adjust the volume and mix well. Note the purity of the Fe(NH4)2(SO4)2.6H20 given on the label of the container. 1.4 Transfer this solution into a 1 L glass bottle. Preparation of working iron standard solution: 1.5 Pipet 10.00 mL of the iron standard stock solution into a 500.0...
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