Extinction coefficient for NADH at 340 nm = 6220 M-1cm-1
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Extinction coefficient for NADH at 340 nm = 6220 M-1cm-1
What is the theoretical absorbance at...
Which of the following pentapeptides will exhibit the highest extinction coefficient at 280 nm? Assume that...
Which of the following pentapeptides will exhibit the highest extinction coefficient at 280 nm? Assume that each tryptophan has an extinction coefficient of 5690 M-1cm-1, tyrosine has an extinction coefficient of 1280 M-1cm-1, and the absorbance of phenylalanine at 280 nm is negligible. TEDSE #TYWSF RYYFE FEWFE The UV-vis absorption spectrum of the peptide RYYFE collected in a 1 cm pathlength cuvette exhibits an absorbance of 0.050 at 280 nm. What is the concentration (in micromolar) of the peptide in...
Calculate the extinction coefficient of PNP in M‒1cm‒1 (p-nitrophenol MW 140 g/mol) if 0.1 g sample...
Calculate the extinction coefficient of PNP in M‒1cm‒1 (p-nitrophenol MW 140 g/mol) if 0.1 g sample is prepared in 500 mL buffer, the above solution produced an absorbance of 4.5 when using a spectrophotometer with a cuvette path length of l = 0.50 cm.
A. A beginning scientist measures an absorbance of 3.0 at 260 nm for a DNA sample....
A. A beginning scientist measures an absorbance of 3.0 at 260 nm for a DNA sample. Is this a meaningful number and why or why not ?(Consider the amount of light passing through the sample, ie. the % transmittance). What range should values of absorbance fall in? B. A 6.0 mg/L solution of adenine in water has an absorbance of 0.59 at 261 nm in a 1 cm pathlength cell. Adenine has a molecular weight of 135.1 g/mol.What is the...
Given the fact that the molar extinction coefficient at 470 nm is 6800 cm-1 M-1 and...
Given the fact that the molar extinction coefficient at 470 nm is 6800 cm-1 M-1 and the radius of the 4-dram vial is 1.00 cm, what would be the absorbance for the highest standard solution (25.00 mL) and for the lowest standard (5.00 mL)? Preparation of iron standard solution (0.01 mg/mL): Weigh (to the nearest 0.1 mg) 0.0350 g of reagent grade (NH4)2Fe(SO4)2•6 H2O into a 500-mL volumetric flask. Dissolve in 250 mL of water that contains ~1 mL of...
Human myoglobin is a protein with a molar extinction coefficient at 280nm of 1.40 x 104...
Human myoglobin is a protein with a molar extinction coefficient at 280nm of 1.40 x 104 M-1cm-1 . 100.0 uL of the stock solution of myoglobin (the tube was marked as 0.050 mg/mL) was mixed with 0.900 mL of buffer, giving an absorbance at 280nm of 0.810 (cuvette is 1.00 cm pathlength). From this information, what is the molecular weight of human myoglobin? Do you think this is a reasonable value? Explain WHY or WHY NOT?
The molar absorptivity of FD&C Yellow #5 at 430 nm is =2.43*104 M-1cm-1. The absorbance of...
The molar absorptivity of FD&C Yellow #5 at 430 nm is
=2.43*104 M-1cm-1. The absorbance
of a sample of Gatorade Lemon-Lime at 430 nm is measured as 0.27
using a 1 cm cuvette. Assuming the path length is 1 cm, calculate
the molarity of Yellow #5 in Gatorade.
Question 3 10 points Save Ans It is important that you understand how to use the data generated b...
Question 3 10 points Save Ans It is important that you understand how to use the data generated by an absorbance based enzyme assay and relate this back to the enzyme activity Using the steps provided below. calculate the actvity of GPOH in the original preparation expressed as units ml tt two decimal places) under the following conditions: 75 uL glucose 6-phosphate dehydrogenase (G6PDH) was assayed in a reaction mixture with a total volume of 1.20 mL. The reaction was...
The absorption coefficient of bovine immunoglobulin at 280 nm is 210,000 M-1 cm-1 . what is...
The absorption coefficient of bovine immunoglobulin at 280 nm is 210,000 M-1 cm-1 . what is the absorbance of a 0.1 mg ml-1 solution across a 1 cm path? what percentage of the incident light is transmitted by this solution? mw (150,000 D)
Spectrophotometry help!? Would measuring the absorbance of the standard solutions used to prepare a calibration curve...
Spectrophotometry help!? Would measuring the absorbance of the standard solutions used to prepare a calibration curve and the unknown sample using a wavelength 10 nm higher or lower than lambda max provide an accurate concentration value for the unknown? Explain. If in an experiment, the pathlength of the light is 1 cm, and the concentration unit of the standard solutions is reported as mM, what would the units of the molar extinction coefficient, ε, be?
The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist...
The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FD&C Blue Dye #1 solution of unknown concentration was 0.295. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express the answer in micromolar (notice micro means 10-6) Concentration of Blue Dye (M) Absorbance...
The molar absorptivity of FD&C Yellow #5 at 430 nm is
=2.43*104 M-1cm-1. The absorbance
of a sample of Gatorade Lemon-Lime at 430 nm is measured as 0.27
using a 1 cm cuvette. Assuming the path length is 1 cm, calculate
the molarity of Yellow #5 in Gatorade.
Question 3 10 points Save Ans It is important that you understand how to use the data generated by an absorbance based enzyme assay and relate this back to the enzyme activity Using the steps provided below. calculate the actvity of GPOH in the original preparation expressed as units ml tt two decimal places) under the following conditions: 75 uL glucose 6-phosphate dehydrogenase (G6PDH) was assayed in a reaction mixture with a total volume of 1.20 mL. The reaction was...
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