The pigment mentioned in the question is showing its maximum absorption at 450nm wavelength. So while constructing a standard curve 450nm wavelength light will be appropriate. So the correct answer is 450nm.
Absorption [%] 300 400 500 600 700 nm What wavelength of light would be the best...
1.2 1.0 0.8 0.6 0.4+ 0.2 0+ 300 350 400 450 500 550 600 650 700 Wavelength (nm) The absorption spectrum of a certain red dye is shown above. If a student analyzing the same concentration of this dye neglected to wipe fingerprints off the cuvette before placing it in the spectrophotometer, how would the absorption curve be affected? Absorbance
Absorbance of chlorophyll a -Rate of photosynthesis 400 500 600 700 Wavelength of light (nm) The graph above shows the absorption spectrum for chlorophyll a. According to the data, which wavelength of light is most effective in driving photosynthesis? A. 420 nm B. 575 nm C.625 nm D.730 nm
udent Name Report on Laboratory Experiment "Spectra" Continuous Spectra Incandescent 400 300 500 600 Fluorescent 800 300 400 500 Bright-line Spectra 600 800 HydrogenmtTT 800 700 600 500 300 Helium 800 700 600 500 400 300 Neon 600 300 Argon 600 500 Wavelength, nm 400 300 QUESTIONS: 1. What is a spectrum? 2. ls white light a simple color or misture of a number of colors? All emission spectra are characteristic of the particular elements. That is, they are like...
08 06 04 Absorbance 02 400 500 600 700 800 Wavelength (nm) For the absorbance spectrum shown, determine the analytical wavelength. 370 nm 400 nm 450 nm o 505 nm 540 nm ОО 580 nm
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Light of wavelength 550 nm is incident on an eye with the sensitivity curve shown. 2 How many type I receptors are activated? Tries 0/5 How many type II receptors are activated? Tries 0/5 How many type III receptors are activated? Tries 0/5 Submit Answer Submit Answer Submit Answer 30 r Type I Type 25 Type III 20 O 15 10 400 450 500 550 600 650 700 Wavelength of Light
Light of wavelength 550 nm...
Chlorophyll a Absorption Spectrum of Visible Light Absorption of light (%) Which of the following wavelengths of light are not absorbed by chlorophyll a to power photosynthesis? Check all that apply. 430 nm 660 nm 530 nm 400 450 500 550 600 Wavelength (nm) 650 700 wavelength (nm) In a laboratory experiment, you take three plants and put them each under a lamp that mimics sunlight. You have three colored light filters that fit over each of the lamps: a...
(i) State which wavelength (primary peak) corresponds to fluorescence and also to phosphorescence? By calculating the energy of each peak, draw the energy level diagram corresponding to the graph below. Highlight transition of light absorbance, fluorescence and phosphorescence emission Briefly state with appropriate diagrams, a) why the phosphorescence emission has a longer lifetime and b) appears at a lower energy with a lower intensity. (ii) (iii) 900 800 700 600 500 400 300 200 100 350 400 450 500 550...
5(15%) Shown below are the absorption and fluorescence spectra for an antibody in pH 72 buffer: 350 400 450 500 550 600 650 700 750 Wavelength (nm) a. Which is the fluorescence spectrum, the red one or the blue one? Explain. b. What is the best wavelength at which to excite this sample in order to study its fluorescence? Explain. c. What is the best wavelength at which to monitor the fluorescence of this sample for quantitative purposes? Explain. d....
Questions 1. Consider the calibration graph and the absorption spectrum for solutions of metal ion, M * Absorption Spectrum for M Calibration Curve for M 0.8 0.7 1.2 0.6 1 0.5 S 0.8 0.4 0.6 0.3 0.4 0.2 0.1 0.2 0- 0 0.5 0.4 0.3 0.2 0.1 400 450 500 550 600 650 700 [M*1 (Molar) (入) nm a) At what wavelength was the calibration curve obtained? Explain your answer. b) Use the calibration curve to calculate the concentration (M)...
Light with a wavelength of 500 nm is shone on a metal and
photoelectrons are produced with non-zero kinetic energy. Light
with a different wavelength of 450 nm is shone on the same metal.
Which of the following is true?
Light with a wavelength of 500 nm is shone on a metal and photoelectrons are produced with non-zero kinetic energy. Light with a different wavelength of 450 nm is shone on the same metal. Which of the following is true?...