Homework Answers
(a)
Red spectrum is fluorescence spectrum since fluorensce alway come more than absorption.
(b)
550 nm is the best wavelength to study the experiment
(c)
580 nm is the best wavelength for quantitative purpose
(d)
580 nm is the longest wavelength coul be excited and still produce its entire fluorescence spectrum.
Add Answer to:
5(15%) Shown below are the absorption and fluorescence spectra for an antibody in pH 72 buffer:...
Use the absorption and fluorescence spectra of bacteriochlorophyll a dissolved in toluene to estimate: (a) the...
Use the absorption and fluorescence spectra of bacteriochlorophyll a dissolved in toluene to estimate: (a) the Stokes shift of the fluorescence spectrum (b) the frequency (in cm−1) of the vibrational mode that is displaced (stretched) by the optical transition from the ground state to the excited state. Compare the estimates you get from the vibrational structure of the absorption and fluorescence spectra. What part of the bacteriochlorophyll macrocycle is likely to be changing structure?Explain. (c) the energy gaps in cm−1...
Structure of quinine. Refer to these diagrams wben answering the following questions. a. Which sp...
please do all the 4 parts neat and clean thanks
structure of quinine. Refer to these diagrams wben answering the following questions. a. Which spectrum (red or blue) is the absorption spectrum? Which spectrum is the fluorescence spectrum? Rationalize your choices. b. What is the best wavelength at which to excite quinine in order to observe its entire fluorescence spectrum? Why? c. What is the best wavelength at which to monitor the fluorescence of quinine-containing solutions? Why? part of the...
3 4 5 6 7 8 9 10 1112 13 14 15 16 17 18 Fluorescence...
3 4 5 6 7 8 9 10 1112 13 14 15 16 17 18 Fluorescence emission 1. Alexa Fluor 405 - 2. Alexa Fluor 350 3. Alexa Fluor 500- 4. Alexa Fluor 488 5. Alexa Fluor 430 6. Alexa Fluor 514 – 7. Alexa Fluor 532 8. Alexa Fluor 555 - 9. Alexa Fluor 546 - 10. Alexa Fluor 568 - 11. Alexa Fluor 594 - 12. Alexa Fluor 610 - 13. Alexa Fluor 633 - 14. Alexa Fluor...
2) An organic diprotic acid has an absorption spectrum that depends on pH. The spectra shown belo...
2) An organic diprotic acid has an absorption spectrum that depends on pH. The spectra shown below show the absorbance of three solutions with different pH values. Each solution had a concentration of 0.0100M. The cell pathlength is 1.00 cm. The dissociation constants of the acid are Ki 103 and K2-1011 a) What are the equilibrium concentrations of the H2A, HA, and A2 forms at each pH? b) What is the molar absorptivity of each form at 630 nm? c)...
2) An organic diprotic acid has an absorption spectrum that depends on pH. The spectra shown...
2) An organic diprotic acid has an absorption spectrum that depends on pH. The spectra shown below show the absorbance of three solutions with different pH values. Each solution had a concentration of 0.0100M. The cell pathlength is 1.00 cm. The dissociation constants of the acid are Ki 103 and K2-1011 a) What are the equilibrium concentrations of the H2A, HA, and A2 forms at each pH? b) What is the molar absorptivity of each form at 630 nm? c)...
The electronic absorption spectrum UI (Cr(NH3)6]3+ (aq) is shown in Figure 11.5. One of the...
The electronic absorption spectrum UI (Cr(NH3)6]3+ (aq) is shown in Figure 11.5. One of the observed peaks is centered at about 303 nm. 1.a. What is the approximate value of the wavelength for the two other peaks? Which one of these three peaks falls in the visible region of the electromagnetic spectrum? The wavelength at this peak is (Amax), and this transition accounts for the color of the complex ion in solution. The energy of the transition occurring at...
The characteristic absorption spectra of two stars are shown. Indicated in each is a set of absor...
The characteristic absorption spectra of two stars are shown.
Indicated in each is a set of absorption lines due to interstellar
material between us and the stars. Using this information and the
spectral drawings shown below complete parts a - e of this
question.
a. The interstellar absorption lines appear more intense in the
spectrum of star A than star B. What does this mean in terms of the
number of absorbing atoms between the earth observer's line of
sight...
Beer lambert law. Simple Q Can some one find the molar absorbitiy "e" for the 1,1'-diethyl 2,2'-cyanine...
Beer lambert law. Simple Q
Can some one find the molar absorbitiy "e" for
the 1,1'-diethyl 2,2'-cyanine iodide. ( SEE DATA BELLOW
AND CALCULATIONS) and the theoretical value and explain where there
is a diffrence between them. Thank you so much.
3) Compare the experimental and literature values of ε (dye I only). Do you notice any discrepancies between these values? Dilution 1 Dilution2 Absorbance Dilution 3 Dilution 4 Dilutions 350 400 450 500 550 600 650 700 750 -0.2 Wavelength...
(5 questions) Data: Consider the data below for a Beer's Law calibration curve for an aqueous...
(5 questions) Data: Consider the data below for a Beer's Law calibration curve for an aqueous orange nye solution that has mas 450 nm. The solutions used were translucent. The data was collected using a 1.20cm wide test tube cuvette, using 2 = 450 nm. Trial Concentration (M) Absorbance Percent Transmittance 1 0 .0030 0.153 70.3 0.0060 0.312 48.8 0.0090 0.465 34.3 0.0120 0.627 23.6 0.0150 0.771 16.9 For each of the following errors/changes, indicate whether a repeat of the...
Absorption and transmission: When a photon of light hits a molecule of gas or a particle...
Absorption and transmission: When a photon of light hits a molecule of gas or a particle in the atmosphere, there are three possibilities: 1) it can be absorbed (the energy is converted to heat), 2) it can be reflected (it continues on but in a different direction), 3) or it can be transmitted. These processes determine our ability to see distant objects (such as the mountains around Los Angeles and Irvine) and the colors in the sky (such as blue...
please do all the 4 parts neat and clean thanks
structure of quinine. Refer to these diagrams wben answering the following questions. a. Which spectrum (red or blue) is the absorption spectrum? Which spectrum is the fluorescence spectrum? Rationalize your choices. b. What is the best wavelength at which to excite quinine in order to observe its entire fluorescence spectrum? Why? c. What is the best wavelength at which to monitor the fluorescence of quinine-containing solutions? Why? part of the...
3 4 5 6 7 8 9 10 1112 13 14 15 16 17 18 Fluorescence emission 1. Alexa Fluor 405 - 2. Alexa Fluor 350 3. Alexa Fluor 500- 4. Alexa Fluor 488 5. Alexa Fluor 430 6. Alexa Fluor 514 – 7. Alexa Fluor 532 8. Alexa Fluor 555 - 9. Alexa Fluor 546 - 10. Alexa Fluor 568 - 11. Alexa Fluor 594 - 12. Alexa Fluor 610 - 13. Alexa Fluor 633 - 14. Alexa Fluor...
2) An organic diprotic acid has an absorption spectrum that depends on pH. The spectra shown below show the absorbance of three solutions with different pH values. Each solution had a concentration of 0.0100M. The cell pathlength is 1.00 cm. The dissociation constants of the acid are Ki 103 and K2-1011 a) What are the equilibrium concentrations of the H2A, HA, and A2 forms at each pH? b) What is the molar absorptivity of each form at 630 nm? c)...
2) An organic diprotic acid has an absorption spectrum that depends on pH. The spectra shown below show the absorbance of three solutions with different pH values. Each solution had a concentration of 0.0100M. The cell pathlength is 1.00 cm. The dissociation constants of the acid are Ki 103 and K2-1011 a) What are the equilibrium concentrations of the H2A, HA, and A2 forms at each pH? b) What is the molar absorptivity of each form at 630 nm? c)...
The electronic absorption spectrum UI (Cr(NH3)6]3+ (aq) is shown in Figure 11.5. One of the observed peaks is centered at about 303 nm. 1.a. What is the approximate value of the wavelength for the two other peaks? Which one of these three peaks falls in the visible region of the electromagnetic spectrum? The wavelength at this peak is (Amax), and this transition accounts for the color of the complex ion in solution. The energy of the transition occurring at...
The characteristic absorption spectra of two stars are shown.
Indicated in each is a set of absorption lines due to interstellar
material between us and the stars. Using this information and the
spectral drawings shown below complete parts a - e of this
question.
a. The interstellar absorption lines appear more intense in the
spectrum of star A than star B. What does this mean in terms of the
number of absorbing atoms between the earth observer's line of
sight...
Beer lambert law. Simple Q
Can some one find the molar absorbitiy "e" for
the 1,1'-diethyl 2,2'-cyanine iodide. ( SEE DATA BELLOW
AND CALCULATIONS) and the theoretical value and explain where there
is a diffrence between them. Thank you so much.
3) Compare the experimental and literature values of ε (dye I only). Do you notice any discrepancies between these values? Dilution 1 Dilution2 Absorbance Dilution 3 Dilution 4 Dilutions 350 400 450 500 550 600 650 700 750 -0.2 Wavelength...
(5 questions) Data: Consider the data below for a Beer's Law calibration curve for an aqueous orange nye solution that has mas 450 nm. The solutions used were translucent. The data was collected using a 1.20cm wide test tube cuvette, using 2 = 450 nm. Trial Concentration (M) Absorbance Percent Transmittance 1 0 .0030 0.153 70.3 0.0060 0.312 48.8 0.0090 0.465 34.3 0.0120 0.627 23.6 0.0150 0.771 16.9 For each of the following errors/changes, indicate whether a repeat of the...
Absorption and transmission: When a photon of light hits a molecule of gas or a particle in the atmosphere, there are three possibilities: 1) it can be absorbed (the energy is converted to heat), 2) it can be reflected (it continues on but in a different direction), 3) or it can be transmitted. These processes determine our ability to see distant objects (such as the mountains around Los Angeles and Irvine) and the colors in the sky (such as blue...
Most questions answered within 3 hours.
-
A 1400Kg sports car accelerates from rest to 90km/h in 7.0s.
What is the average power...
asked 34 minutes ago -
For the following reaction, 0.128 moles of
potassium hydrogen sulfateare mixed with
0.504 moles of potassium...
asked 4 hours ago -
1. What is the present value of $400, three years in the future
if the interest...
asked 4 hours ago -
The labor force minus the number of employed equals the number
of unemployed.
a. True
b....
asked 6 hours ago -
Determine the mass in units of grams [g] of 0.49 moles [mol]
of a new fictitious...
asked 7 hours ago -
A horizontal mass of M=5kg is on a spring and stretched to
x=0.5m when released from...
asked 8 hours ago -
26 of 50
"I have worked at the Arizona Humane Society for ten years, and
have...
asked 8 hours ago -
Compare and contrast zero based budgeting and incremental (or
base year) budgeting.
asked 8 hours ago -
4 pts 10. Which of the following hypothesis would be MOST
difficult to test experimentally? Group...
asked 8 hours ago -
A business owner makes 1,000 items a day. Each day he or she
contributes eight hours...
asked 8 hours ago -
A
circular loop in the plane of a paper lies inca0.65 T magnetic
field pointing into...
asked 9 hours ago -
A business owner is trying to decide whether to buy, rent, or
lease office space and...
asked 9 hours ago